Simultaneous determination of tryptophan, 5-hydroxytryptophan, tryptamine, serotonin, and 5-HIAA in small volumes of mouse serum using UHPLC-ED

Anthony Gallegos, Roslyn Rivkah Isseroff

Research output: Contribution to journalArticlepeer-review

Abstract

In this paper we report a simple and efficient method for the concurrent analysis of tryptophan, 5-HTP, tryptamine, serotonin, and 5-HIAA in mouse serum using UHPLC-ED after protein precipitation and dilution. These compounds are neuroactive and are of interest in studies of mood and behavior; They are also biomarkers for the presence of neuroendocrine tumors and are used in the diagnosis of these cancers. After a brief series of validation experiments, this method was applied to serum from mouse behaviour experiments. • A convenient UHPLC method with electrochemical detection for concomitant analysis of the serotonin pathway in serum, including, for the first time, tryptamine. • The method met all performance criteria established for use in our lab and was applied in rodent experiments.

Original languageEnglish (US)
Article number101624
JournalMethodsX
Volume9
DOIs
StatePublished - Jan 2022
Externally publishedYes

Keywords

  • Behavior
  • Biogenic amines
  • Chromatographic analysis
  • Chromatographic analysis of tryptophan, 5-hydroxytryptophan, tryptamine, serotonin, and 5-HIAA in small volumes of mouse serum
  • Electrochemical detection
  • Indoleamines
  • Mood
  • Neuroendocrine tumors
  • Protein precipitation
  • Serotonin
  • Serum measurement

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medical Laboratory Technology

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