Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining

Ezra Orlofsky, Osnat Gillor, Ann Melli, Woutrina A Smith, Stefan Wuertz, Nirit Bernstein, Karen Shapiro

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

An improved approach for simultaneous detection of Cryptosporidium parvum and Giardia lamblia (oo)cysts in soil is described. Recoveries>70% were obtained for concentrations>55 and 21 (oo)cysts g-1 for C. parvum and G. lamblia, respectively. The limits of detection were determined to be<5 (oo)cysts g-1 soil.

Original languageEnglish (US)
Pages (from-to)375-377
Number of pages3
JournalJournal of Microbiological Methods
Volume94
Issue number3
DOIs
StatePublished - Sep 2013

Keywords

  • Cryptosporidium parvum
  • Detection
  • Giardia lamblia
  • Immunomagnetic separation
  • Recovery
  • Soil contamination

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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