Simultaneous detection of endogenous lectins and their binding capacity at the single-cell level - A technical note

V. Froňková, Z. Holíková, Fu-Tong Liu, J. Homolka, D. C. Rijken, S. André, N. V. Bovin, K. Smetana, H. J. Gabius

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Endogenous lectins are proteins/glycoproteins which selectively recognize distinct saccharide ligands and are different from immunoglobulins and carbohydrate-utilizing enzymes. Expression of these molecules can be detected immunohistochemically using nonblocking monoclonal antibodies (A1D6: anti-galectin-3, MR-15-2-2: anti-175 kD mannose receptor). Alternatively, biotinylated (neo)glycoconjugates which are recognized by a studied lectin can be employed as convenient probes to demonstrate specific binding of sugar epitopes by carbohydrate recognition domains (CRD) of endogenous lectins. In this study, we describe a new procedure for immunocytochemical visualization of the expression of endogenous lectins and glycochemical visualization of the reactivity of carbohydrate recognition domain(s), performed simultaneously at the single-cell level.

Original languageEnglish (US)
Pages (from-to)157-162
Number of pages6
JournalFolia Biologica
Volume45
Issue number4
StatePublished - 1999

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

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