Simultaneous detection of endogenous lectins and their binding capacity at the single-cell level - A technical note

V. Froňková; Z. Holíková; Fu-Tong Liu; J. Homolka; D. C. Rijken; S. André; N. V. Bovin; K. Smetana; H. J. Gabius

Simultaneous detection of endogenous lectins and their binding capacity at the single-cell level - A technical note

V. Froňková, Z. Holíková, Fu-Tong Liu, J. Homolka, D. C. Rijken, S. André, N. V. Bovin, K. Smetana, H. J. Gabius

Dermatology

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Endogenous lectins are proteins/glycoproteins which selectively recognize distinct saccharide ligands and are different from immunoglobulins and carbohydrate-utilizing enzymes. Expression of these molecules can be detected immunohistochemically using nonblocking monoclonal antibodies (A1D6: anti-galectin-3, MR-15-2-2: anti-175 kD mannose receptor). Alternatively, biotinylated (neo)glycoconjugates which are recognized by a studied lectin can be employed as convenient probes to demonstrate specific binding of sugar epitopes by carbohydrate recognition domains (CRD) of endogenous lectins. In this study, we describe a new procedure for immunocytochemical visualization of the expression of endogenous lectins and glycochemical visualization of the reactivity of carbohydrate recognition domain(s), performed simultaneously at the single-cell level.

Original language	English (US)
Pages (from-to)	157-162
Number of pages	6
Journal	Folia Biologica
Volume	45
Issue number	4
State	Published - 1999

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Agricultural and Biological Sciences (miscellaneous)

Fingerprint Dive into the research topics of 'Simultaneous detection of endogenous lectins and their binding capacity at the single-cell level - A technical note'. Together they form a unique fingerprint.

Cite this

@article{8ff367ea41974ace8d7c1ade1b025138,

title = "Simultaneous detection of endogenous lectins and their binding capacity at the single-cell level - A technical note",

abstract = "Endogenous lectins are proteins/glycoproteins which selectively recognize distinct saccharide ligands and are different from immunoglobulins and carbohydrate-utilizing enzymes. Expression of these molecules can be detected immunohistochemically using nonblocking monoclonal antibodies (A1D6: anti-galectin-3, MR-15-2-2: anti-175 kD mannose receptor). Alternatively, biotinylated (neo)glycoconjugates which are recognized by a studied lectin can be employed as convenient probes to demonstrate specific binding of sugar epitopes by carbohydrate recognition domains (CRD) of endogenous lectins. In this study, we describe a new procedure for immunocytochemical visualization of the expression of endogenous lectins and glycochemical visualization of the reactivity of carbohydrate recognition domain(s), performed simultaneously at the single-cell level.",

author = "V. Fro{\v n}kov{\'a} and Z. Hol{\'i}kov{\'a} and Fu-Tong Liu and J. Homolka and Rijken, {D. C.} and S. Andr{\'e} and Bovin, {N. V.} and K. Smetana and Gabius, {H. J.}",

year = "1999",

language = "English (US)",

volume = "45",

pages = "157--162",

journal = "Ceskoslovenská biologie",

issn = "0015-5500",

publisher = "Institute of Systematics and Evolution of Animals",

number = "4",

}

TY - JOUR

T1 - Simultaneous detection of endogenous lectins and their binding capacity at the single-cell level - A technical note

AU - Froňková, V.

AU - Holíková, Z.

AU - Liu, Fu-Tong

AU - Homolka, J.

AU - Rijken, D. C.

AU - André, S.

AU - Bovin, N. V.

AU - Smetana, K.

AU - Gabius, H. J.

PY - 1999

Y1 - 1999

N2 - Endogenous lectins are proteins/glycoproteins which selectively recognize distinct saccharide ligands and are different from immunoglobulins and carbohydrate-utilizing enzymes. Expression of these molecules can be detected immunohistochemically using nonblocking monoclonal antibodies (A1D6: anti-galectin-3, MR-15-2-2: anti-175 kD mannose receptor). Alternatively, biotinylated (neo)glycoconjugates which are recognized by a studied lectin can be employed as convenient probes to demonstrate specific binding of sugar epitopes by carbohydrate recognition domains (CRD) of endogenous lectins. In this study, we describe a new procedure for immunocytochemical visualization of the expression of endogenous lectins and glycochemical visualization of the reactivity of carbohydrate recognition domain(s), performed simultaneously at the single-cell level.

AB - Endogenous lectins are proteins/glycoproteins which selectively recognize distinct saccharide ligands and are different from immunoglobulins and carbohydrate-utilizing enzymes. Expression of these molecules can be detected immunohistochemically using nonblocking monoclonal antibodies (A1D6: anti-galectin-3, MR-15-2-2: anti-175 kD mannose receptor). Alternatively, biotinylated (neo)glycoconjugates which are recognized by a studied lectin can be employed as convenient probes to demonstrate specific binding of sugar epitopes by carbohydrate recognition domains (CRD) of endogenous lectins. In this study, we describe a new procedure for immunocytochemical visualization of the expression of endogenous lectins and glycochemical visualization of the reactivity of carbohydrate recognition domain(s), performed simultaneously at the single-cell level.

UR - http://www.scopus.com/inward/record.url?scp=0032810737&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032810737&partnerID=8YFLogxK

M3 - Article

C2 - 10732730

AN - SCOPUS:0032810737

VL - 45

SP - 157

EP - 162

JO - Ceskoslovenská biologie

JF - Ceskoslovenská biologie

SN - 0015-5500

IS - 4

ER -

Simultaneous detection of endogenous lectins and their binding capacity at the single-cell level - A technical note

Abstract

ASJC Scopus subject areas

Other files and links

Cite this