Abstract
A one-step method for conjugating macrocyclic chelators to antibodies using the protein modification reagent 2-iminothiolane controls aggregation, maintains immunoreactivity, and produces consistent chelate/antibody ratios. Conjugation conditions have been investigated with the macrocyclic chelates 6-[p-(bromoacetamido)benzyl]-1,4,8,11-tetraazacyclotetradecane-N,N′, N″,N‴-tetraacetic acid and 2-[p-(bromoacetamido)benzyl]-1,4,7,10-tetraazacydododecane-N,N′,N″, N′-tetraacetic acid, with three different monoclonal antibodies. The bifunctional chelating agents are prepared by bromoacetylation of their amine precursors using a two-phase H2O/CHCl3 system, which improves product purity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 222-226 |
| Number of pages | 5 |
| Journal | Bioconjugate Chemistry |
| Volume | 1 |
| Issue number | 3 |
| State | Published - 1990 |
ASJC Scopus subject areas
- Chemistry(all)
- Organic Chemistry
- Clinical Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry