Simple step gradient elution of the major high-energy compounds and their catabolites in cardiac muscle using high-performance liquid chromatography

Emily A. Hull-ryde, William R Lewis, Charles D. Veronee, James E. Lowe

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

The majority of high-energy nucleotides and their catabolites are separated in a single 13-min run using reversed-phase high-performance liquid chromatography. Economical step gradient elution of these compounds with a Nova Pak-A C18, 5-μm, 10 cm X 8 mm column accomplishes this separation with recoveries of 94-100% and sensitivities of 1-5 pmol. Furthermore, the total adenine nucleotide pool can now be quantitated precisely in a simple and easily automated procedure.

Original languageEnglish (US)
Pages (from-to)165-174
Number of pages10
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume377
Issue numberC
DOIs
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine
  • Analytical Chemistry

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