Abstract
This paper describes the development of a simple system for measurement of net water movement through biological membrane barriers. The system is based on the detection of a water meniscus inside a polyethylene tube, which reflects the water movement inside one hemichamber of a modified Ussing chamber containing a membrane barrier. The detection device consists of a commercial computer-controlled flat bed scanner and specifically developed software. This system allows one to perform a relatively high number of individual experiments per physical unit. It is a flexible and affordable device, which allows comparatively more information per unit to be obtained than previously described methods.
Original language | English (US) |
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Pages (from-to) | 95-100 |
Number of pages | 6 |
Journal | Computer Methods and Programs in Biomedicine |
Volume | 85 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2007 |
Keywords
- Clostridium perfringens
- Epithelia
- Ussing chamber
- Water transport
ASJC Scopus subject areas
- Software