Abstract
This paper describes the development of a simple system for measurement of net water movement through biological membrane barriers. The system is based on the detection of a water meniscus inside a polyethylene tube, which reflects the water movement inside one hemichamber of a modified Ussing chamber containing a membrane barrier. The detection device consists of a commercial computer-controlled flat bed scanner and specifically developed software. This system allows one to perform a relatively high number of individual experiments per physical unit. It is a flexible and affordable device, which allows comparatively more information per unit to be obtained than previously described methods.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-100 |
| Number of pages | 6 |
| Journal | Computer Methods and Programs in Biomedicine |
| Volume | 85 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2007 |
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Keywords
- Clostridium perfringens
- Epithelia
- Ussing chamber
- Water transport
ASJC Scopus subject areas
- Software
Cite this
Simple multichannel system for the measurement of the net water flux across biological tissues. / Fernández-Miyakawa, Mariano E.; Dorr, Ricardo; Fernández, Luis E.; Uzal, Francisco A; Ibarra, Cristina.
In: Computer Methods and Programs in Biomedicine, Vol. 85, No. 2, 02.2007, p. 95-100.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simple multichannel system for the measurement of the net water flux across biological tissues
AU - Fernández-Miyakawa, Mariano E.
AU - Dorr, Ricardo
AU - Fernández, Luis E.
AU - Uzal, Francisco A
AU - Ibarra, Cristina
PY - 2007/2
Y1 - 2007/2
N2 - This paper describes the development of a simple system for measurement of net water movement through biological membrane barriers. The system is based on the detection of a water meniscus inside a polyethylene tube, which reflects the water movement inside one hemichamber of a modified Ussing chamber containing a membrane barrier. The detection device consists of a commercial computer-controlled flat bed scanner and specifically developed software. This system allows one to perform a relatively high number of individual experiments per physical unit. It is a flexible and affordable device, which allows comparatively more information per unit to be obtained than previously described methods.
AB - This paper describes the development of a simple system for measurement of net water movement through biological membrane barriers. The system is based on the detection of a water meniscus inside a polyethylene tube, which reflects the water movement inside one hemichamber of a modified Ussing chamber containing a membrane barrier. The detection device consists of a commercial computer-controlled flat bed scanner and specifically developed software. This system allows one to perform a relatively high number of individual experiments per physical unit. It is a flexible and affordable device, which allows comparatively more information per unit to be obtained than previously described methods.
KW - Clostridium perfringens
KW - Epithelia
KW - Ussing chamber
KW - Water transport
UR - http://www.scopus.com/inward/record.url?scp=33846187204&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846187204&partnerID=8YFLogxK
U2 - 10.1016/j.cmpb.2006.09.016
DO - 10.1016/j.cmpb.2006.09.016
M3 - Article
C2 - 17113182
AN - SCOPUS:33846187204
VL - 85
SP - 95
EP - 100
JO - Computer Methods and Programs in Biomedicine
JF - Computer Methods and Programs in Biomedicine
SN - 0169-2607
IS - 2
ER -