Simple multichannel system for the measurement of the net water flux across biological tissues

Mariano E. Fernández-Miyakawa; Ricardo Dorr; Luis E. Fernández; Francisco A Uzal; Cristina Ibarra

doi:10.1016/j.cmpb.2006.09.016

Simple multichannel system for the measurement of the net water flux across biological tissues

Mariano E. Fernández-Miyakawa, Ricardo Dorr, Luis E. Fernández, Francisco A Uzal, Cristina Ibarra

California Animal Health & Food Safety Lab

Research output: Contribution to journal › Article

5 Scopus citations

Abstract

This paper describes the development of a simple system for measurement of net water movement through biological membrane barriers. The system is based on the detection of a water meniscus inside a polyethylene tube, which reflects the water movement inside one hemichamber of a modified Ussing chamber containing a membrane barrier. The detection device consists of a commercial computer-controlled flat bed scanner and specifically developed software. This system allows one to perform a relatively high number of individual experiments per physical unit. It is a flexible and affordable device, which allows comparatively more information per unit to be obtained than previously described methods.

Original language	English (US)
Pages (from-to)	95-100
Number of pages	6
Journal	Computer Methods and Programs in Biomedicine
Volume	85
Issue number	2
DOIs	https://doi.org/10.1016/j.cmpb.2006.09.016
State	Published - Feb 2007

Keywords

Clostridium perfringens
Epithelia
Ussing chamber
Water transport

ASJC Scopus subject areas

Software

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Fernández-Miyakawa, M. E., Dorr, R., Fernández, L. E., Uzal, F. A., & Ibarra, C. (2007). Simple multichannel system for the measurement of the net water flux across biological tissues. Computer Methods and Programs in Biomedicine, 85(2), 95-100. https://doi.org/10.1016/j.cmpb.2006.09.016

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