Simple multichannel system for the measurement of the net water flux across biological tissues

Mariano E. Fernández-Miyakawa, Ricardo Dorr, Luis E. Fernández, Francisco A Uzal, Cristina Ibarra

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

This paper describes the development of a simple system for measurement of net water movement through biological membrane barriers. The system is based on the detection of a water meniscus inside a polyethylene tube, which reflects the water movement inside one hemichamber of a modified Ussing chamber containing a membrane barrier. The detection device consists of a commercial computer-controlled flat bed scanner and specifically developed software. This system allows one to perform a relatively high number of individual experiments per physical unit. It is a flexible and affordable device, which allows comparatively more information per unit to be obtained than previously described methods.

Original languageEnglish (US)
Pages (from-to)95-100
Number of pages6
JournalComputer Methods and Programs in Biomedicine
Volume85
Issue number2
DOIs
StatePublished - Feb 2007

Keywords

  • Clostridium perfringens
  • Epithelia
  • Ussing chamber
  • Water transport

ASJC Scopus subject areas

  • Software

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