Simian retrovirus-D serotype 1 (SRV-1) envelope glycoproteins gp70 and gp20

Expression in yeast cells and identification of specific antibodies in sera from monkeys that recovered from SRV-1 infection

H. S. Kwang, P. J. Barr, E. A. Sabin, S. Sujipto, P. A. Marx, M. D. Power, I. C. Bathurst, Niels C Pedersen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The gp70 and transmembrane gp20 envelope proteins of simian retrovirus-D serotype 1 (SRV-1) were expressed in Saccharomyces cerevisiae as fusion proteins with human superoxide dismutase (SOD). Expression of the SOD-gp70 and SOD-gp20 sequences yielded fusion proteins of 52 and 29 kilodaltons, respectively. The yeast-expressed SRV-1 envelope proteins were used in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in the sera of rhesus macaques that recovered from SRV-1. Sera from 47 of 49 such monkeys tested positive for antibodies to the SOD-gp70 fusion protein, while 45 of 49 reacted positively to SOD-gp20. None of 26 SRV-1-nonexposed monkeys tested positive in either ELISA. Monkeys immunized with the recombinant SRV-1 gp20 and gp70 proteins made good ELISA and Western blot (immunoblot) antibodies to whole SRV-1. This antibody was not neutralizing in vitro, however.

Original languageEnglish (US)
Pages (from-to)1774-1780
Number of pages7
JournalJournal of Virology
Volume62
Issue number5
StatePublished - 1988

Fingerprint

Simian Retroviruses
Retroviridae
Haplorhini
monkeys
glycoproteins
Glycoproteins
serotypes
Yeasts
Superoxide Dismutase
superoxide dismutase
yeasts
antibodies
Antibodies
Infection
Serum
infection
proteins
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
cells

ASJC Scopus subject areas

  • Immunology

Cite this

Simian retrovirus-D serotype 1 (SRV-1) envelope glycoproteins gp70 and gp20 : Expression in yeast cells and identification of specific antibodies in sera from monkeys that recovered from SRV-1 infection. / Kwang, H. S.; Barr, P. J.; Sabin, E. A.; Sujipto, S.; Marx, P. A.; Power, M. D.; Bathurst, I. C.; Pedersen, Niels C.

In: Journal of Virology, Vol. 62, No. 5, 1988, p. 1774-1780.

Research output: Contribution to journalArticle

@article{92da4ca84d9e42ee96d78e4b759ee853,
title = "Simian retrovirus-D serotype 1 (SRV-1) envelope glycoproteins gp70 and gp20: Expression in yeast cells and identification of specific antibodies in sera from monkeys that recovered from SRV-1 infection",
abstract = "The gp70 and transmembrane gp20 envelope proteins of simian retrovirus-D serotype 1 (SRV-1) were expressed in Saccharomyces cerevisiae as fusion proteins with human superoxide dismutase (SOD). Expression of the SOD-gp70 and SOD-gp20 sequences yielded fusion proteins of 52 and 29 kilodaltons, respectively. The yeast-expressed SRV-1 envelope proteins were used in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in the sera of rhesus macaques that recovered from SRV-1. Sera from 47 of 49 such monkeys tested positive for antibodies to the SOD-gp70 fusion protein, while 45 of 49 reacted positively to SOD-gp20. None of 26 SRV-1-nonexposed monkeys tested positive in either ELISA. Monkeys immunized with the recombinant SRV-1 gp20 and gp70 proteins made good ELISA and Western blot (immunoblot) antibodies to whole SRV-1. This antibody was not neutralizing in vitro, however.",
author = "Kwang, {H. S.} and Barr, {P. J.} and Sabin, {E. A.} and S. Sujipto and Marx, {P. A.} and Power, {M. D.} and Bathurst, {I. C.} and Pedersen, {Niels C}",
year = "1988",
language = "English (US)",
volume = "62",
pages = "1774--1780",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Simian retrovirus-D serotype 1 (SRV-1) envelope glycoproteins gp70 and gp20

T2 - Expression in yeast cells and identification of specific antibodies in sera from monkeys that recovered from SRV-1 infection

AU - Kwang, H. S.

AU - Barr, P. J.

AU - Sabin, E. A.

AU - Sujipto, S.

AU - Marx, P. A.

AU - Power, M. D.

AU - Bathurst, I. C.

AU - Pedersen, Niels C

PY - 1988

Y1 - 1988

N2 - The gp70 and transmembrane gp20 envelope proteins of simian retrovirus-D serotype 1 (SRV-1) were expressed in Saccharomyces cerevisiae as fusion proteins with human superoxide dismutase (SOD). Expression of the SOD-gp70 and SOD-gp20 sequences yielded fusion proteins of 52 and 29 kilodaltons, respectively. The yeast-expressed SRV-1 envelope proteins were used in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in the sera of rhesus macaques that recovered from SRV-1. Sera from 47 of 49 such monkeys tested positive for antibodies to the SOD-gp70 fusion protein, while 45 of 49 reacted positively to SOD-gp20. None of 26 SRV-1-nonexposed monkeys tested positive in either ELISA. Monkeys immunized with the recombinant SRV-1 gp20 and gp70 proteins made good ELISA and Western blot (immunoblot) antibodies to whole SRV-1. This antibody was not neutralizing in vitro, however.

AB - The gp70 and transmembrane gp20 envelope proteins of simian retrovirus-D serotype 1 (SRV-1) were expressed in Saccharomyces cerevisiae as fusion proteins with human superoxide dismutase (SOD). Expression of the SOD-gp70 and SOD-gp20 sequences yielded fusion proteins of 52 and 29 kilodaltons, respectively. The yeast-expressed SRV-1 envelope proteins were used in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in the sera of rhesus macaques that recovered from SRV-1. Sera from 47 of 49 such monkeys tested positive for antibodies to the SOD-gp70 fusion protein, while 45 of 49 reacted positively to SOD-gp20. None of 26 SRV-1-nonexposed monkeys tested positive in either ELISA. Monkeys immunized with the recombinant SRV-1 gp20 and gp70 proteins made good ELISA and Western blot (immunoblot) antibodies to whole SRV-1. This antibody was not neutralizing in vitro, however.

UR - http://www.scopus.com/inward/record.url?scp=0023906091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023906091&partnerID=8YFLogxK

M3 - Article

VL - 62

SP - 1774

EP - 1780

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 5

ER -