Simian retrovirus-D serotype 1 (SRV-1) envelope glycoproteins gp70 and gp20: Expression in yeast cells and identification of specific antibodies in sera from monkeys that recovered from SRV-1 infection

H. S. Kwang, P. J. Barr, E. A. Sabin, S. Sujipto, P. A. Marx, M. D. Power, I. C. Bathurst, Niels C Pedersen

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Abstract

The gp70 and transmembrane gp20 envelope proteins of simian retrovirus-D serotype 1 (SRV-1) were expressed in Saccharomyces cerevisiae as fusion proteins with human superoxide dismutase (SOD). Expression of the SOD-gp70 and SOD-gp20 sequences yielded fusion proteins of 52 and 29 kilodaltons, respectively. The yeast-expressed SRV-1 envelope proteins were used in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in the sera of rhesus macaques that recovered from SRV-1. Sera from 47 of 49 such monkeys tested positive for antibodies to the SOD-gp70 fusion protein, while 45 of 49 reacted positively to SOD-gp20. None of 26 SRV-1-nonexposed monkeys tested positive in either ELISA. Monkeys immunized with the recombinant SRV-1 gp20 and gp70 proteins made good ELISA and Western blot (immunoblot) antibodies to whole SRV-1. This antibody was not neutralizing in vitro, however.

Original languageEnglish (US)
Pages (from-to)1774-1780
Number of pages7
JournalJournal of Virology
Volume62
Issue number5
StatePublished - 1988

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ASJC Scopus subject areas

  • Immunology

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