Abstract
The gp70 and transmembrane gp20 envelope proteins of simian retrovirus-D serotype 1 (SRV-1) were expressed in Saccharomyces cerevisiae as fusion proteins with human superoxide dismutase (SOD). Expression of the SOD-gp70 and SOD-gp20 sequences yielded fusion proteins of 52 and 29 kilodaltons, respectively. The yeast-expressed SRV-1 envelope proteins were used in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in the sera of rhesus macaques that recovered from SRV-1. Sera from 47 of 49 such monkeys tested positive for antibodies to the SOD-gp70 fusion protein, while 45 of 49 reacted positively to SOD-gp20. None of 26 SRV-1-nonexposed monkeys tested positive in either ELISA. Monkeys immunized with the recombinant SRV-1 gp20 and gp70 proteins made good ELISA and Western blot (immunoblot) antibodies to whole SRV-1. This antibody was not neutralizing in vitro, however.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1774-1780 |
| Number of pages | 7 |
| Journal | Journal of Virology |
| Volume | 62 |
| Issue number | 5 |
| State | Published - 1988 |
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ASJC Scopus subject areas
- Immunology
Cite this
Simian retrovirus-D serotype 1 (SRV-1) envelope glycoproteins gp70 and gp20 : Expression in yeast cells and identification of specific antibodies in sera from monkeys that recovered from SRV-1 infection. / Kwang, H. S.; Barr, P. J.; Sabin, E. A.; Sujipto, S.; Marx, P. A.; Power, M. D.; Bathurst, I. C.; Pedersen, Niels C.
In: Journal of Virology, Vol. 62, No. 5, 1988, p. 1774-1780.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simian retrovirus-D serotype 1 (SRV-1) envelope glycoproteins gp70 and gp20
T2 - Expression in yeast cells and identification of specific antibodies in sera from monkeys that recovered from SRV-1 infection
AU - Kwang, H. S.
AU - Barr, P. J.
AU - Sabin, E. A.
AU - Sujipto, S.
AU - Marx, P. A.
AU - Power, M. D.
AU - Bathurst, I. C.
AU - Pedersen, Niels C
PY - 1988
Y1 - 1988
N2 - The gp70 and transmembrane gp20 envelope proteins of simian retrovirus-D serotype 1 (SRV-1) were expressed in Saccharomyces cerevisiae as fusion proteins with human superoxide dismutase (SOD). Expression of the SOD-gp70 and SOD-gp20 sequences yielded fusion proteins of 52 and 29 kilodaltons, respectively. The yeast-expressed SRV-1 envelope proteins were used in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in the sera of rhesus macaques that recovered from SRV-1. Sera from 47 of 49 such monkeys tested positive for antibodies to the SOD-gp70 fusion protein, while 45 of 49 reacted positively to SOD-gp20. None of 26 SRV-1-nonexposed monkeys tested positive in either ELISA. Monkeys immunized with the recombinant SRV-1 gp20 and gp70 proteins made good ELISA and Western blot (immunoblot) antibodies to whole SRV-1. This antibody was not neutralizing in vitro, however.
AB - The gp70 and transmembrane gp20 envelope proteins of simian retrovirus-D serotype 1 (SRV-1) were expressed in Saccharomyces cerevisiae as fusion proteins with human superoxide dismutase (SOD). Expression of the SOD-gp70 and SOD-gp20 sequences yielded fusion proteins of 52 and 29 kilodaltons, respectively. The yeast-expressed SRV-1 envelope proteins were used in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in the sera of rhesus macaques that recovered from SRV-1. Sera from 47 of 49 such monkeys tested positive for antibodies to the SOD-gp70 fusion protein, while 45 of 49 reacted positively to SOD-gp20. None of 26 SRV-1-nonexposed monkeys tested positive in either ELISA. Monkeys immunized with the recombinant SRV-1 gp20 and gp70 proteins made good ELISA and Western blot (immunoblot) antibodies to whole SRV-1. This antibody was not neutralizing in vitro, however.
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M3 - Article
VL - 62
SP - 1774
EP - 1780
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 5
ER -