Abstract
Simian foamy viruses, members of the spumavirus subfamily of retroviruses, are found in a variety of nonhuman primates and, as yet, remain to be characterized with respect to genetic structure and regulation of viral gene expression. The genome of simian foamy virus type 1 (SFV-1), an isolate from rhesus macaques, has been molecularly cloned, and the role of the viral long terminal repeat (LTR) in transcriptional control has been investigated. The SFV-1 LTR is 1,621 base pairs long, and sequence comparisons with human foamy virus revealed a pattern of clustered homology. A cap site in the LTR was identified by analysis of SFV-1 transcripts i in infected cells. Transient expression assays in cell lines representing several species and different cell types showed that the SFV-1 LTR has low basal activity in uninfected cells, whereas LTR-directed expression is greatly increased in cells infected with SFV-1. This transactivation is mediated by a mechanism involving functions on the LTR at the transcriptional level. on the LTR at the level.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3598-3604 |
| Number of pages | 7 |
| Journal | Journal of Virology |
| Volume | 64 |
| Issue number | 8 |
| State | Published - 1990 |
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ASJC Scopus subject areas
- Immunology
Cite this
Simian foamy virus type 1 is a retrovirus which encodes a transcriptional transactivator. / Mergia, Ayalew; Shaw, Karen E S; Pratt-Lowe, Elissa; Barry, Peter A; Luciw, Paul A.
In: Journal of Virology, Vol. 64, No. 8, 1990, p. 3598-3604.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Simian foamy virus type 1 is a retrovirus which encodes a transcriptional transactivator
AU - Mergia, Ayalew
AU - Shaw, Karen E S
AU - Pratt-Lowe, Elissa
AU - Barry, Peter A
AU - Luciw, Paul A
PY - 1990
Y1 - 1990
N2 - Simian foamy viruses, members of the spumavirus subfamily of retroviruses, are found in a variety of nonhuman primates and, as yet, remain to be characterized with respect to genetic structure and regulation of viral gene expression. The genome of simian foamy virus type 1 (SFV-1), an isolate from rhesus macaques, has been molecularly cloned, and the role of the viral long terminal repeat (LTR) in transcriptional control has been investigated. The SFV-1 LTR is 1,621 base pairs long, and sequence comparisons with human foamy virus revealed a pattern of clustered homology. A cap site in the LTR was identified by analysis of SFV-1 transcripts i in infected cells. Transient expression assays in cell lines representing several species and different cell types showed that the SFV-1 LTR has low basal activity in uninfected cells, whereas LTR-directed expression is greatly increased in cells infected with SFV-1. This transactivation is mediated by a mechanism involving functions on the LTR at the transcriptional level. on the LTR at the level.
AB - Simian foamy viruses, members of the spumavirus subfamily of retroviruses, are found in a variety of nonhuman primates and, as yet, remain to be characterized with respect to genetic structure and regulation of viral gene expression. The genome of simian foamy virus type 1 (SFV-1), an isolate from rhesus macaques, has been molecularly cloned, and the role of the viral long terminal repeat (LTR) in transcriptional control has been investigated. The SFV-1 LTR is 1,621 base pairs long, and sequence comparisons with human foamy virus revealed a pattern of clustered homology. A cap site in the LTR was identified by analysis of SFV-1 transcripts i in infected cells. Transient expression assays in cell lines representing several species and different cell types showed that the SFV-1 LTR has low basal activity in uninfected cells, whereas LTR-directed expression is greatly increased in cells infected with SFV-1. This transactivation is mediated by a mechanism involving functions on the LTR at the transcriptional level. on the LTR at the level.
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M3 - Article
C2 - 2370676
AN - SCOPUS:0025368666
VL - 64
SP - 3598
EP - 3604
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 8
ER -