Simian foamy virus type 1 is a retrovirus which encodes a transcriptional transactivator

Ayalew Mergia, Karen E S Shaw, Elissa Pratt-Lowe, Peter A Barry, Paul A Luciw

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Simian foamy viruses, members of the spumavirus subfamily of retroviruses, are found in a variety of nonhuman primates and, as yet, remain to be characterized with respect to genetic structure and regulation of viral gene expression. The genome of simian foamy virus type 1 (SFV-1), an isolate from rhesus macaques, has been molecularly cloned, and the role of the viral long terminal repeat (LTR) in transcriptional control has been investigated. The SFV-1 LTR is 1,621 base pairs long, and sequence comparisons with human foamy virus revealed a pattern of clustered homology. A cap site in the LTR was identified by analysis of SFV-1 transcripts i in infected cells. Transient expression assays in cell lines representing several species and different cell types showed that the SFV-1 LTR has low basal activity in uninfected cells, whereas LTR-directed expression is greatly increased in cells infected with SFV-1. This transactivation is mediated by a mechanism involving functions on the LTR at the transcriptional level. on the LTR at the level.

Original languageEnglish (US)
Pages (from-to)3598-3604
Number of pages7
JournalJournal of Virology
Volume64
Issue number8
StatePublished - 1990

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Simian foamy virus
Retroviridae
Spumavirus
terminal repeat sequences
Trans-Activators
Terminal Repeat Sequences
Viral Gene Expression Regulation
cells
Genetic Structures
transcriptional activation
Macaca mulatta
transactivators
Base Pairing
Primates
Transcriptional Activation
cell lines
Genome
Cell Line
gene expression

ASJC Scopus subject areas

  • Immunology

Cite this

Simian foamy virus type 1 is a retrovirus which encodes a transcriptional transactivator. / Mergia, Ayalew; Shaw, Karen E S; Pratt-Lowe, Elissa; Barry, Peter A; Luciw, Paul A.

In: Journal of Virology, Vol. 64, No. 8, 1990, p. 3598-3604.

Research output: Contribution to journalArticle

Mergia, Ayalew ; Shaw, Karen E S ; Pratt-Lowe, Elissa ; Barry, Peter A ; Luciw, Paul A. / Simian foamy virus type 1 is a retrovirus which encodes a transcriptional transactivator. In: Journal of Virology. 1990 ; Vol. 64, No. 8. pp. 3598-3604.
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