Silver nanoparticles in nonlinear microscopy

D. J. Rativa; A. S L Gomes; Sebastian Wachsmann-Hogiu; D. L. Farkas; R. E. De Araujo

doi:10.1109/IMOC.2007.4404309

Silver nanoparticles in nonlinear microscopy

D. J. Rativa, A. S L Gomes, Sebastian Wachsmann-Hogiu, D. L. Farkas, R. E. De Araujo

Pathology and Laboratory Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Nanotechnology has led to the manufacture of nanometer-sized objects which can be explored in multiphoton microscopy. In particular, metal nanoparticles (NPs) do not generate the multiphoton signal by itself, but rather it can enhance the nonlinear (NL) emission of fluorochromes, owing to the influence of local field effects. Energy transfer process is also observed in systems consisting of metal nanoparticles and organic dye molecules, leading to fluorescence quenching caused by an increased nonradiative rate and decrease in the dye's radiative rate. Here we have measured and analyzed the fluorescence of Tryptophan solution with and without silver nanoparticles, excited by one, two and three photon absorption. Due to the molecule interaction with the metallic NPs, fluorescence enhancement and quenching process were observed. We show that silver nanoparticles can be explored as a new tool in fluorescence microscopy.

Original language	English (US)
Title of host publication	SBMO/IEEE MTT-S International Microwave and Optoelectronics Conference Proceedings
Pages	478-482
Number of pages	5
DOIs	https://doi.org/10.1109/IMOC.2007.4404309
State	Published - 2007
Event	2007 SBMO/IEEE MTT-S International Microwave and Optoelectronics Conference, IMOC - Salvador, Brazil Duration: Jan 29 2007 → Nov 1 2007

Other

Other	2007 SBMO/IEEE MTT-S International Microwave and Optoelectronics Conference, IMOC
Country	Brazil
City	Salvador
Period	1/29/07 → 11/1/07

Keywords

Fluorescence
Microscopy
Nanotechnology
Nonlinear optics

ASJC Scopus subject areas

Physics and Astronomy(all)
Engineering(all)

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10.1109/IMOC.2007.4404309

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Rativa, DJ, Gomes, ASL, Wachsmann-Hogiu, S, Farkas, DL & De Araujo, RE 2007, Silver nanoparticles in nonlinear microscopy. in SBMO/IEEE MTT-S International Microwave and Optoelectronics Conference Proceedings., 4404309, pp. 478-482, 2007 SBMO/IEEE MTT-S International Microwave and Optoelectronics Conference, IMOC, Salvador, Brazil, 1/29/07. https://doi.org/10.1109/IMOC.2007.4404309

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abstract = "Nanotechnology has led to the manufacture of nanometer-sized objects which can be explored in multiphoton microscopy. In particular, metal nanoparticles (NPs) do not generate the multiphoton signal by itself, but rather it can enhance the nonlinear (NL) emission of fluorochromes, owing to the influence of local field effects. Energy transfer process is also observed in systems consisting of metal nanoparticles and organic dye molecules, leading to fluorescence quenching caused by an increased nonradiative rate and decrease in the dye's radiative rate. Here we have measured and analyzed the fluorescence of Tryptophan solution with and without silver nanoparticles, excited by one, two and three photon absorption. Due to the molecule interaction with the metallic NPs, fluorescence enhancement and quenching process were observed. We show that silver nanoparticles can be explored as a new tool in fluorescence microscopy.",

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author = "Rativa, {D. J.} and Gomes, {A. S L} and Sebastian Wachsmann-Hogiu and Farkas, {D. L.} and {De Araujo}, {R. E.}",

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AU - Rativa, D. J.

AU - Gomes, A. S L

AU - Wachsmann-Hogiu, Sebastian

AU - Farkas, D. L.

AU - De Araujo, R. E.

PY - 2007

Y1 - 2007

N2 - Nanotechnology has led to the manufacture of nanometer-sized objects which can be explored in multiphoton microscopy. In particular, metal nanoparticles (NPs) do not generate the multiphoton signal by itself, but rather it can enhance the nonlinear (NL) emission of fluorochromes, owing to the influence of local field effects. Energy transfer process is also observed in systems consisting of metal nanoparticles and organic dye molecules, leading to fluorescence quenching caused by an increased nonradiative rate and decrease in the dye's radiative rate. Here we have measured and analyzed the fluorescence of Tryptophan solution with and without silver nanoparticles, excited by one, two and three photon absorption. Due to the molecule interaction with the metallic NPs, fluorescence enhancement and quenching process were observed. We show that silver nanoparticles can be explored as a new tool in fluorescence microscopy.

AB - Nanotechnology has led to the manufacture of nanometer-sized objects which can be explored in multiphoton microscopy. In particular, metal nanoparticles (NPs) do not generate the multiphoton signal by itself, but rather it can enhance the nonlinear (NL) emission of fluorochromes, owing to the influence of local field effects. Energy transfer process is also observed in systems consisting of metal nanoparticles and organic dye molecules, leading to fluorescence quenching caused by an increased nonradiative rate and decrease in the dye's radiative rate. Here we have measured and analyzed the fluorescence of Tryptophan solution with and without silver nanoparticles, excited by one, two and three photon absorption. Due to the molecule interaction with the metallic NPs, fluorescence enhancement and quenching process were observed. We show that silver nanoparticles can be explored as a new tool in fluorescence microscopy.

KW - Fluorescence

KW - Microscopy

KW - Nanotechnology

KW - Nonlinear optics

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