Silk worm Bm1 SINE RNA increases following cellular insults

Richard H. Kimura, Prabhakara V Choudary, Carl W. Schmid

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The effect of cell stresses upon the expression of the Bm1 short interspersed element (SINE) family in cultured silk worm cells is examined. Primer extension analysis shows that Bm1 repeats are transcribed by RNA polymerase III (Pol III) into cytoplasmic RNAs. Five consecutive T residues, which would normally terminate Pol III transcription, occur within the Bm1 consensus and are included within cDNA sequences representing these transcripts. In analogy to mammalian SINEs, the level of the Bm1 transcripts increases in response to either heat shock, inhibiting protein synthesis by cycloheximide or viral infection. The lifetime of Bm1 RNA increases following cell insults so that post-transcriptional events partially account for stress induced increases in its abundance. In the case of heat shock, the increase in Bm1 RNA follows the transient increase in hsp70 mRNA indicating that this response is temporally regulated to occur later in heat shock recovery. These results support the proposal that SINE RNAs serve a role in the cell stress response that predates the divergence of insects and mammals implying that SINEs are essentially a class of cell stress genes.

Original languageEnglish (US)
Pages (from-to)3380-3387
Number of pages8
JournalNucleic Acids Research
Volume27
Issue number16
DOIs
StatePublished - Aug 15 1999

Fingerprint

Silk
RNA
Short Interspersed Nucleotide Elements
Shock
Hot Temperature
RNA Polymerase III
Virus Diseases
Cycloheximide
Heat-Shock Proteins
Prednisolone
Insects
Mammals
Complementary DNA
Messenger RNA
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Silk worm Bm1 SINE RNA increases following cellular insults. / Kimura, Richard H.; Choudary, Prabhakara V; Schmid, Carl W.

In: Nucleic Acids Research, Vol. 27, No. 16, 15.08.1999, p. 3380-3387.

Research output: Contribution to journalArticle

Kimura, RH, Choudary, PV & Schmid, CW 1999, 'Silk worm Bm1 SINE RNA increases following cellular insults', Nucleic Acids Research, vol. 27, no. 16, pp. 3380-3387. https://doi.org/10.1093/nar/27.16.3380
Kimura, Richard H. ; Choudary, Prabhakara V ; Schmid, Carl W. / Silk worm Bm1 SINE RNA increases following cellular insults. In: Nucleic Acids Research. 1999 ; Vol. 27, No. 16. pp. 3380-3387.
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