Signaling via the CD2 receptor enhances HTLV-1 replication in T lymphocytes

Deborah J. Guyot, Garret C. Newbound, Michael Dale Lairmore

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Human T lymphotropic virus type 1 (HTLV-1) is considered the etiologic agent or adult T cell leukemia/lymphoma and several chronic progressive immune-mediated diseases. Approximately 1-4% of infected individuals develop disease, generally decades following infection. Increased proviral transcription, mediated by the viral 40-kDa trans-activating protein, Tax, has been implicated in the pathogenesis of HTLV-l-associated diseases. Since the HTLV-1 promoter contains sequences responsive to cyclic AMP and protein kinase C, we hypothesized that lymphocyte activation signals initiated through the TCR/CD3 complex or CD2 receptor promote viral replication in HTLV-1-infected lymphocytes. We demonstrate that mAbs directed against the CD2, but not the CD3 receptor increase viral p24 capsid protein 1.5- to 5.7-fold in CD2/CD3+ HTLV1-infected cell culture supernatants. Northern blot analysis demonstrated a 2.5- to 4-fold increase in all species of viral mRNA following CD2 cross-linking of OSP2/4 cells, an immortalized HTLV-1 cell line. Consistent with transcriptional regulation, reporter gene activity increased approximately 11-fold in CD2-stimulated Jurkat T cells cotranslected with a Tax-expressing plasmid and a CAT reporter gene construct under control of the HTLV-1 promoter. These data suggest a possible physiologic mechanism, whereby CD2-mediated cell adhesion and lymphocyte activation may promote viral transcription in infected lymphocytes.

Original languageEnglish (US)
Pages (from-to)123-129
Number of pages7
JournalVirology
Volume234
Issue number1
DOIs
StatePublished - Jul 21 1997
Externally publishedYes

Fingerprint

Human T-lymphotropic virus 1
Virus Replication
T-Lymphocytes
Lymphocyte Activation
Reporter Genes
T-Cell Antigen Receptor-CD3 Complex
tax Gene Products
Lymphocytes
Adenylate Kinase
Adult T Cell Leukemia Lymphoma
Jurkat Cells
Immune System Diseases
Capsid Proteins
Cell Adhesion
Northern Blotting
Cyclic AMP
Protein Kinase C
Plasmids
Cell Culture Techniques
Cell Line

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Signaling via the CD2 receptor enhances HTLV-1 replication in T lymphocytes. / Guyot, Deborah J.; Newbound, Garret C.; Lairmore, Michael Dale.

In: Virology, Vol. 234, No. 1, 21.07.1997, p. 123-129.

Research output: Contribution to journalArticle

Guyot, Deborah J. ; Newbound, Garret C. ; Lairmore, Michael Dale. / Signaling via the CD2 receptor enhances HTLV-1 replication in T lymphocytes. In: Virology. 1997 ; Vol. 234, No. 1. pp. 123-129.
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