Abstract
The authors demonstrate Raman-resonant imaging based on the simultaneous generation of several nonlinear frequency mixing processes resulting from a 3-color coherent anti-Stokes Raman scattering (CARS) experiment. The interaction of three coincident short-pulsed laser beams simultaneously generates both 2-color (degenerate) CARS and 3-color (non-degenerate) CARS signals, which are collected and characterized spectroscopically - allowing for resonant, doubly-resonant, and non-resonant contrast mechanisms. Images obtained from both 2-color and 3-color CARS signals are compared and found to provide complementary information. The 3-color CARS microscopy scheme provides a versatile multiplexed modality for biological imaging, which may extend the capabilities of label-free non-linear microscopy, e.g. by probing multiple Raman resonances.Doubly-resonant difference CARS image of a section of a C. elegans nematode imaged at the CD resonance (upper half) and the CH2 resonance (lower half).
Original language | English (US) |
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Pages (from-to) | 169-175 |
Number of pages | 7 |
Journal | Journal of Biophotonics |
Volume | 3 |
Issue number | 3 |
DOIs | |
State | Published - Mar 2010 |
Keywords
- CARS
- Four-wave mixing
- Microscopy
- Nonlinear optics
- Raman scattering
ASJC Scopus subject areas
- Materials Science(all)
- Engineering(all)
- Physics and Astronomy(all)
- Chemistry(all)
- Biochemistry, Genetics and Molecular Biology(all)