Abstract
The authors demonstrate Raman-resonant imaging based on the simultaneous generation of several nonlinear frequency mixing processes resulting from a 3-color coherent anti-Stokes Raman scattering (CARS) experiment. The interaction of three coincident short-pulsed laser beams simultaneously generates both 2-color (degenerate) CARS and 3-color (non-degenerate) CARS signals, which are collected and characterized spectroscopically - allowing for resonant, doubly-resonant, and non-resonant contrast mechanisms. Images obtained from both 2-color and 3-color CARS signals are compared and found to provide complementary information. The 3-color CARS microscopy scheme provides a versatile multiplexed modality for biological imaging, which may extend the capabilities of label-free non-linear microscopy, e.g. by probing multiple Raman resonances.Doubly-resonant difference CARS image of a section of a C. elegans nematode imaged at the CD resonance (upper half) and the CH2 resonance (lower half).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 169-175 |
| Number of pages | 7 |
| Journal | Journal of Biophotonics |
| Volume | 3 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 2010 |
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Keywords
- CARS
- Four-wave mixing
- Microscopy
- Nonlinear optics
- Raman scattering
ASJC Scopus subject areas
- Materials Science(all)
- Engineering(all)
- Physics and Astronomy(all)
- Chemistry(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Signal generation and Raman-resonant imaging by non-degenerate four-wave mixing under tight focusing conditions. / Weeks, Tyler; Schie, Iwan W.; Wachsmann-Hogiu, Sebastian; Huser, Thomas R.
In: Journal of Biophotonics, Vol. 3, No. 3, 03.2010, p. 169-175.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Signal generation and Raman-resonant imaging by non-degenerate four-wave mixing under tight focusing conditions
AU - Weeks, Tyler
AU - Schie, Iwan W.
AU - Wachsmann-Hogiu, Sebastian
AU - Huser, Thomas R
PY - 2010/3
Y1 - 2010/3
N2 - The authors demonstrate Raman-resonant imaging based on the simultaneous generation of several nonlinear frequency mixing processes resulting from a 3-color coherent anti-Stokes Raman scattering (CARS) experiment. The interaction of three coincident short-pulsed laser beams simultaneously generates both 2-color (degenerate) CARS and 3-color (non-degenerate) CARS signals, which are collected and characterized spectroscopically - allowing for resonant, doubly-resonant, and non-resonant contrast mechanisms. Images obtained from both 2-color and 3-color CARS signals are compared and found to provide complementary information. The 3-color CARS microscopy scheme provides a versatile multiplexed modality for biological imaging, which may extend the capabilities of label-free non-linear microscopy, e.g. by probing multiple Raman resonances.Doubly-resonant difference CARS image of a section of a C. elegans nematode imaged at the CD resonance (upper half) and the CH2 resonance (lower half).
AB - The authors demonstrate Raman-resonant imaging based on the simultaneous generation of several nonlinear frequency mixing processes resulting from a 3-color coherent anti-Stokes Raman scattering (CARS) experiment. The interaction of three coincident short-pulsed laser beams simultaneously generates both 2-color (degenerate) CARS and 3-color (non-degenerate) CARS signals, which are collected and characterized spectroscopically - allowing for resonant, doubly-resonant, and non-resonant contrast mechanisms. Images obtained from both 2-color and 3-color CARS signals are compared and found to provide complementary information. The 3-color CARS microscopy scheme provides a versatile multiplexed modality for biological imaging, which may extend the capabilities of label-free non-linear microscopy, e.g. by probing multiple Raman resonances.Doubly-resonant difference CARS image of a section of a C. elegans nematode imaged at the CD resonance (upper half) and the CH2 resonance (lower half).
KW - CARS
KW - Four-wave mixing
KW - Microscopy
KW - Nonlinear optics
KW - Raman scattering
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U2 - 10.1002/jbio.200900068
DO - 10.1002/jbio.200900068
M3 - Article
VL - 3
SP - 169
EP - 175
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 3
ER -