Short-term zinc deficiency affects nuclear factor-κB nuclear binding activity in rat testes

Patricia I. Oteiza, Michael S. Clegg, Carl L Keen

Research output: Contribution to journalArticle

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Abstract

We reported previously that feeding zinc-deficient diets for 14 d altered the oxidant defense system in the testes of young male rats and increased levels of lipid, protein and DNA oxidation in this tissue. In this study, we investigated the early involvement of oxidative stress in zinc deficiency-induced testicular pathology. Weanling male rats (17 d old) were given free access to a control (25 μg Zn/g) or a zinc-deficient (0.5 μg Zn/g) diet, or restricted access to the control diet at a level of intake similar to that of rats fed the 0.5 μg Zn/g diet (restricted group) for 7 d. Rats fed the low zinc diet were characterized by low testes zinc and alkaline phosphatase activity compared with ad libitum and restricted controls. Testes protein and lipid oxidation variables did not differ among the groups. Higher than normal (P < 0.05) activities of CuZn (CuZnSOD) and Mn (MnSOD) superoxide dismutases were observed in the low zinc group. Glutathione peroxidase and glutathione reductase activities did not differ among the groups. Total glutathione concentrations were lower in the low zinc and restricted groups than in the control group (P < 0.05). The testes nuclear binding activities of two transcription factors sensitive to oxidants [nuclear factor (NF)-κB and AP-1] were assessed. AP-1 nuclear binding activity did not differ among the groups, but NF-κB nuclear binding activity was lower in the low zinc group than in the control groups (P < 0.05). We suggest that the reduction in NF-κB binding reflects an early response to zinc deficiency-induced oxidative stress.

Original languageEnglish (US)
Pages (from-to)21-26
Number of pages6
JournalJournal of Nutrition
Volume131
Issue number1
StatePublished - 2001

Fingerprint

Testis
Zinc
testes
zinc
rats
Diet
diet
Transcription Factor AP-1
Oxidants
oxidants
Oxidative Stress
oxidative stress
Lipids
Control Groups
Glutathione Reductase
Glutathione Peroxidase
glutathione-disulfide reductase
weanlings
glutathione peroxidase
Superoxide Dismutase

Keywords

  • AP-1
  • Nuclear factor-κB
  • Oxidative stress
  • Rats
  • Testes
  • Zinc deficiency

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Short-term zinc deficiency affects nuclear factor-κB nuclear binding activity in rat testes. / Oteiza, Patricia I.; Clegg, Michael S.; Keen, Carl L.

In: Journal of Nutrition, Vol. 131, No. 1, 2001, p. 21-26.

Research output: Contribution to journalArticle

Oteiza, Patricia I. ; Clegg, Michael S. ; Keen, Carl L. / Short-term zinc deficiency affects nuclear factor-κB nuclear binding activity in rat testes. In: Journal of Nutrition. 2001 ; Vol. 131, No. 1. pp. 21-26.
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