Short-term exercise training increases ACh-induced relaxation and eNOS protein in porcine pulmonary arteries

Lynelle R Johnson, James W E Rush, James R. Turk, Elmer M. Price, M. Harold Laughlin

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

We tested the hypothesis that short-term exercise (STEx) training and the associated increase in pulmonary blood flow during bouts of exercise cause enhanced endothelium-dependent vasorelaxation in porcine pulmonary arteries and increased expression of endothelial cell nitric oxide synthase (eNOS) and superoxide dismutase-1 (SOD-1) protein. Mature, female Yucatan miniature swine exercised I h twice daily on a motorized treadmill for 1 wk (STEx group, n = 7); control pigs (Sed, n = 6) were kept in pens. Pulmonary arteries were isolated from the left caudal lung lobe, and vasomotor responses were determined in vitro. Arterial tissue from the distal portion of this pulmonary artery was processed for immunoblot analysis. Maximal endothelium-dependent (ACh-stimulated) relaxation was greater in STEx (71 ± 5%) than in Sed (44 ± 6%) arteries (P < 0.05), and endothelium-independent (sodium nitroprusside-mediated) responses did not differ. Sensitivity to ACh was not altered by STEx training. Immunoblot analysis indicated a 3.9-fold increase in eNOS protein in pulmonary artery tissue from STEx pigs (P < 0.05) with no change in SOD-1 or glyceraldehyde-3-phosphate dehydrogenase protein levels. We conclude that STEx training enhances ACh-stimulated vasorelaxation in pulmonary arterial tissue and that this adaptation is associated with increased expression of eNOS protein.

Original languageEnglish (US)
Pages (from-to)1102-1110
Number of pages9
JournalJournal of Applied Physiology
Volume90
Issue number3
StatePublished - 2001

    Fingerprint

Keywords

  • Acetylcholine
  • Endothelium
  • Nitric oxide synthase
  • Pulmonary circulation
  • Superoxide dismutase

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this