Short-term early exposure to thirdhand cigarette smoke increases lung cancer incidence in mice

Bo Hang, Yunshan Wang, Yurong Huang, Pin Wang, Sasha A. Langley, Lei Bi, Altaf H. Sarker, Suzaynn F. Schick, Christopher Havel, Peyton Jacob, Neal Benowitz, Hugo Destaillats, Xiaochen Tang, Yankai Xia, Kuang Yu Jen, Lara A. Gundel, Jian Hua Mao, Antoine M. Snijders

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Exposure to thirdhand smoke (THS) is a recently described health concern that arises in many indoor environments. However, the carcinogenic potential of THS, a critical consideration in risk assessment, remains untested. Here we investigated the effects of short-term early exposure to THS on lung carcinogenesis in A/J mice. Forty weeks after THS exposure from 4 to 7 weeks of age, the mice had increased incidence of lung adenocarcinoma, tumor size and, multiplicity, compared with controls. In vitro studies using cultured human lung cancer cells showed that THS exposure induced DNA double-strand breaks and increased cell proliferation and colony formation. RNA sequencing analysis revealed that THS exposure induced endoplasmic reticulum stress and activated p53 signaling. Activation of the p53 pathway was confirmed by an increase in its targets p21 and BAX. These data indicate that early exposure to THS is associated with increased lung cancer risk.

Original languageEnglish (US)
Pages (from-to)475-488
Number of pages14
JournalClinical Science
Issue number4
StatePublished - Jan 1 2018
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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