Abstract
During the monitoring of arbovirus seroprevalence in wild birds collected in California, we inadvertently made two isolates of western equine encephalomyelitis virus (WEEV) from California quail sera being tested by plaque reduction neutralization assay for antibodies against St Louis encephalitis (SLEV) and West Nile (WNV) viruses despite heating the sera at 56°C for 30 minutes. These data prompted us to examine the thermostability of these viruses during heat treatment. The flaviviruses, SLEV and WNV, at titers up to 10 6 plaque-forming units (PFU), were readily inactivated by the standard protocol of heating at 56°C for 30 minutes. In contrast, solutions containing 10 5 and 10 6 PFU of WEEV required 2 hours for complete inactivation. Occasional presence of live virus within sera could lead to false negatives using standard plaque reduction neutralization test protocols.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 862-863 |
| Number of pages | 2 |
| Journal | American Journal of Tropical Medicine and Hygiene |
| Volume | 80 |
| Issue number | 5 |
| State | Published - May 1 2009 |
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ASJC Scopus subject areas
- Parasitology
- Virology
- Medicine(all)
- Infectious Diseases
Cite this
Short report : Comparative thermostability of west nile, St. Louis encephalitis, and western equine encephalomyelitis viruses during heat inactivation for serologic diagnostics. / Fang, Ying; Brault, Aaron; Reisen, William.
In: American Journal of Tropical Medicine and Hygiene, Vol. 80, No. 5, 01.05.2009, p. 862-863.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Short report
T2 - Comparative thermostability of west nile, St. Louis encephalitis, and western equine encephalomyelitis viruses during heat inactivation for serologic diagnostics
AU - Fang, Ying
AU - Brault, Aaron
AU - Reisen, William
PY - 2009/5/1
Y1 - 2009/5/1
N2 - During the monitoring of arbovirus seroprevalence in wild birds collected in California, we inadvertently made two isolates of western equine encephalomyelitis virus (WEEV) from California quail sera being tested by plaque reduction neutralization assay for antibodies against St Louis encephalitis (SLEV) and West Nile (WNV) viruses despite heating the sera at 56°C for 30 minutes. These data prompted us to examine the thermostability of these viruses during heat treatment. The flaviviruses, SLEV and WNV, at titers up to 10 6 plaque-forming units (PFU), were readily inactivated by the standard protocol of heating at 56°C for 30 minutes. In contrast, solutions containing 10 5 and 10 6 PFU of WEEV required 2 hours for complete inactivation. Occasional presence of live virus within sera could lead to false negatives using standard plaque reduction neutralization test protocols.
AB - During the monitoring of arbovirus seroprevalence in wild birds collected in California, we inadvertently made two isolates of western equine encephalomyelitis virus (WEEV) from California quail sera being tested by plaque reduction neutralization assay for antibodies against St Louis encephalitis (SLEV) and West Nile (WNV) viruses despite heating the sera at 56°C for 30 minutes. These data prompted us to examine the thermostability of these viruses during heat treatment. The flaviviruses, SLEV and WNV, at titers up to 10 6 plaque-forming units (PFU), were readily inactivated by the standard protocol of heating at 56°C for 30 minutes. In contrast, solutions containing 10 5 and 10 6 PFU of WEEV required 2 hours for complete inactivation. Occasional presence of live virus within sera could lead to false negatives using standard plaque reduction neutralization test protocols.
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UR - http://www.scopus.com/inward/citedby.url?scp=66149091949&partnerID=8YFLogxK
M3 - Article
C2 - 19407138
AN - SCOPUS:66149091949
VL - 80
SP - 862
EP - 863
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
SN - 0002-9637
IS - 5
ER -