Short report: Comparative thermostability of west nile, St. Louis encephalitis, and western equine encephalomyelitis viruses during heat inactivation for serologic diagnostics

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Abstract

During the monitoring of arbovirus seroprevalence in wild birds collected in California, we inadvertently made two isolates of western equine encephalomyelitis virus (WEEV) from California quail sera being tested by plaque reduction neutralization assay for antibodies against St Louis encephalitis (SLEV) and West Nile (WNV) viruses despite heating the sera at 56°C for 30 minutes. These data prompted us to examine the thermostability of these viruses during heat treatment. The flaviviruses, SLEV and WNV, at titers up to 10 6 plaque-forming units (PFU), were readily inactivated by the standard protocol of heating at 56°C for 30 minutes. In contrast, solutions containing 10 5 and 10 6 PFU of WEEV required 2 hours for complete inactivation. Occasional presence of live virus within sera could lead to false negatives using standard plaque reduction neutralization test protocols.

Original languageEnglish (US)
Pages (from-to)862-863
Number of pages2
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume80
Issue number5
StatePublished - May 1 2009

Fingerprint

St. Louis Encephalitis
Western Equine Encephalitis Viruses
Hot Temperature
Heating
Serum
Viruses
Arboviruses
Flavivirus
Neutralization Tests
West Nile virus
Quail
Seroepidemiologic Studies
Encephalitis
Birds
Antibodies

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Medicine(all)
  • Infectious Diseases

Cite this

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abstract = "During the monitoring of arbovirus seroprevalence in wild birds collected in California, we inadvertently made two isolates of western equine encephalomyelitis virus (WEEV) from California quail sera being tested by plaque reduction neutralization assay for antibodies against St Louis encephalitis (SLEV) and West Nile (WNV) viruses despite heating the sera at 56°C for 30 minutes. These data prompted us to examine the thermostability of these viruses during heat treatment. The flaviviruses, SLEV and WNV, at titers up to 10 6 plaque-forming units (PFU), were readily inactivated by the standard protocol of heating at 56°C for 30 minutes. In contrast, solutions containing 10 5 and 10 6 PFU of WEEV required 2 hours for complete inactivation. Occasional presence of live virus within sera could lead to false negatives using standard plaque reduction neutralization test protocols.",
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