Short communication: Identification of coagulase-negative staphylococcus species from goat milk with the API Staph identification test and with transfer RNA-intergenic spacer PCR combined with capillary electrophoresis

G. Koop, A. De Visscher, C. A. Collar, D. A C Bacon, E. A. Maga, J. D. Murray, K. Supré, S. De Vliegher, F. Haesebrouck, Joan D Rowe, M. Nielen, T. van Werven

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Coagulase-negative staphylococci (CNS) are the most commonly isolated bacteria from goat milk, but they have often been identified with phenotypic methods, which may have resulted in misclassification. The aims of this paper were to assess the amount of misclassification of a phenotypic test for identifying CNS species from goat milk compared with transfer RNA intergenic spacer PCR (tDNA-PCR) followed by capillary electrophoresis, and to apply the tDNA-PCR technique on different capillary electrophoresis equipment. Milk samples were collected from 416 does in 5 Californian dairy goat herds on 3 occasions during lactation. In total, 219 CNS isolates were identified at the species level with tDNA-PCR and subjected to the API 20 Staph identification test kit (API Staph; bioMérieux, Durham, NC). If the same species was isolated multiple times from the same udder gland, only the first isolate was used for further analyses, resulting in 115 unique CNS isolates. According to the tDNA-PCR test, the most prevalent CNS species were Staphylococcus epidermidis, Staphylococcus caprae, and Staphylococcus simulans. Typeability with API staph was low (72%). Although the API Staph test was capable of identifying the majority of Staph. epidermidis and Staph. caprae isolates, sensitivity for identification of Staph. simulans was low. The true positive fraction was high for the 3 most prevalent species. It was concluded that the overall performance of API Staph in differentiating CNS species from goat milk was moderate to low, mainly because of the low typeability, and that genotypic methods such as tDNA-PCR are preferred.

Original languageEnglish (US)
Pages (from-to)7200-7205
Number of pages6
JournalJournal of Dairy Science
Volume95
Issue number12
DOIs
StatePublished - Dec 2012

Fingerprint

transfer RNA
coagulase negative staphylococci
capillary electrophoresis
Coagulase
goat milk
Capillary Electrophoresis
Transfer RNA
intergenic DNA
Staphylococcus
Goats
Milk
Communication
Polymerase Chain Reaction
testing
Staphylococcus caprae
Staphylococcus simulans
Capra
Staphylococcus epidermidis
analytical kits
dairy goats

Keywords

  • API 20 Staph identification test
  • Coagulase-negative Staphylococcus species
  • Goat
  • Transfer DNA-PCR

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Science
  • Genetics

Cite this

Short communication : Identification of coagulase-negative staphylococcus species from goat milk with the API Staph identification test and with transfer RNA-intergenic spacer PCR combined with capillary electrophoresis. / Koop, G.; De Visscher, A.; Collar, C. A.; Bacon, D. A C; Maga, E. A.; Murray, J. D.; Supré, K.; De Vliegher, S.; Haesebrouck, F.; Rowe, Joan D; Nielen, M.; van Werven, T.

In: Journal of Dairy Science, Vol. 95, No. 12, 12.2012, p. 7200-7205.

Research output: Contribution to journalArticle

Koop, G. ; De Visscher, A. ; Collar, C. A. ; Bacon, D. A C ; Maga, E. A. ; Murray, J. D. ; Supré, K. ; De Vliegher, S. ; Haesebrouck, F. ; Rowe, Joan D ; Nielen, M. ; van Werven, T. / Short communication : Identification of coagulase-negative staphylococcus species from goat milk with the API Staph identification test and with transfer RNA-intergenic spacer PCR combined with capillary electrophoresis. In: Journal of Dairy Science. 2012 ; Vol. 95, No. 12. pp. 7200-7205.
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abstract = "Coagulase-negative staphylococci (CNS) are the most commonly isolated bacteria from goat milk, but they have often been identified with phenotypic methods, which may have resulted in misclassification. The aims of this paper were to assess the amount of misclassification of a phenotypic test for identifying CNS species from goat milk compared with transfer RNA intergenic spacer PCR (tDNA-PCR) followed by capillary electrophoresis, and to apply the tDNA-PCR technique on different capillary electrophoresis equipment. Milk samples were collected from 416 does in 5 Californian dairy goat herds on 3 occasions during lactation. In total, 219 CNS isolates were identified at the species level with tDNA-PCR and subjected to the API 20 Staph identification test kit (API Staph; bioM{\'e}rieux, Durham, NC). If the same species was isolated multiple times from the same udder gland, only the first isolate was used for further analyses, resulting in 115 unique CNS isolates. According to the tDNA-PCR test, the most prevalent CNS species were Staphylococcus epidermidis, Staphylococcus caprae, and Staphylococcus simulans. Typeability with API staph was low (72{\%}). Although the API Staph test was capable of identifying the majority of Staph. epidermidis and Staph. caprae isolates, sensitivity for identification of Staph. simulans was low. The true positive fraction was high for the 3 most prevalent species. It was concluded that the overall performance of API Staph in differentiating CNS species from goat milk was moderate to low, mainly because of the low typeability, and that genotypic methods such as tDNA-PCR are preferred.",
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AU - Bacon, D. A C

AU - Maga, E. A.

AU - Murray, J. D.

AU - Supré, K.

AU - De Vliegher, S.

AU - Haesebrouck, F.

AU - Rowe, Joan D

AU - Nielen, M.

AU - van Werven, T.

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