Abstract
Antibodies to human brain, liver, and choroid plexus were produced by immunization of rabbits with fresh autopsy material in Freund's complete adjuvant, and then were absorbed with human AB cells. Rabbit anti-human brain serum was cytotoxic for peripheral blood T cells using the two-stage microcytotoxicity assay. In the presence of complement, it prevented formation of E rosettes and reduced responsiveness to Con A, PHA-P, and allogeneic cells. In the absence of complement, it was strongly mitogenic, much like rabbit anti-human thymocyte sera. Similarly, rabbit anti-human-brain sera could be absorbed with human peripheral blood T cells, but not with B cells or liver. Rabbit anti-human-liver and choroid plexus sera did not exhibit such T-cell specificity. Lymphocytotoxic antibodies were also studied in the cerebrospinal fluid of 11 patients with systemic lupus erythematosus, and of 31 neurologic disease controls. There was no correlation in SLE with presence of CNS disease and either titer or target cell specificity of lymphocytotoxic antibodies. In contrast, patients with multiple sclerosis or meningitis, possessing CSF lymphocytotoxic antibodies, had specificities directed only against T cells, suggesting their origin as autoimmunization with inflamed brain.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 269-280 |
| Number of pages | 12 |
| Journal | Clinical Immunology and Immunopathology |
| Volume | 7 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1977 |
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ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Pathology and Forensic Medicine
Cite this
Shared antigenic determinants between rabbit antihuman brain and rabbit antihuman thymocyte sera : Relationship to the lymphocytotoxic antibodies of systemic lupus erythematosus. / Huntley, Arthur C.; Fletcher, Mark P.; Ikeda, Richard M.; Gershwin, M. Eric.
In: Clinical Immunology and Immunopathology, Vol. 7, No. 2, 1977, p. 269-280.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Shared antigenic determinants between rabbit antihuman brain and rabbit antihuman thymocyte sera
T2 - Relationship to the lymphocytotoxic antibodies of systemic lupus erythematosus
AU - Huntley, Arthur C.
AU - Fletcher, Mark P.
AU - Ikeda, Richard M.
AU - Gershwin, M. Eric
PY - 1977
Y1 - 1977
N2 - Antibodies to human brain, liver, and choroid plexus were produced by immunization of rabbits with fresh autopsy material in Freund's complete adjuvant, and then were absorbed with human AB cells. Rabbit anti-human brain serum was cytotoxic for peripheral blood T cells using the two-stage microcytotoxicity assay. In the presence of complement, it prevented formation of E rosettes and reduced responsiveness to Con A, PHA-P, and allogeneic cells. In the absence of complement, it was strongly mitogenic, much like rabbit anti-human thymocyte sera. Similarly, rabbit anti-human-brain sera could be absorbed with human peripheral blood T cells, but not with B cells or liver. Rabbit anti-human-liver and choroid plexus sera did not exhibit such T-cell specificity. Lymphocytotoxic antibodies were also studied in the cerebrospinal fluid of 11 patients with systemic lupus erythematosus, and of 31 neurologic disease controls. There was no correlation in SLE with presence of CNS disease and either titer or target cell specificity of lymphocytotoxic antibodies. In contrast, patients with multiple sclerosis or meningitis, possessing CSF lymphocytotoxic antibodies, had specificities directed only against T cells, suggesting their origin as autoimmunization with inflamed brain.
AB - Antibodies to human brain, liver, and choroid plexus were produced by immunization of rabbits with fresh autopsy material in Freund's complete adjuvant, and then were absorbed with human AB cells. Rabbit anti-human brain serum was cytotoxic for peripheral blood T cells using the two-stage microcytotoxicity assay. In the presence of complement, it prevented formation of E rosettes and reduced responsiveness to Con A, PHA-P, and allogeneic cells. In the absence of complement, it was strongly mitogenic, much like rabbit anti-human thymocyte sera. Similarly, rabbit anti-human-brain sera could be absorbed with human peripheral blood T cells, but not with B cells or liver. Rabbit anti-human-liver and choroid plexus sera did not exhibit such T-cell specificity. Lymphocytotoxic antibodies were also studied in the cerebrospinal fluid of 11 patients with systemic lupus erythematosus, and of 31 neurologic disease controls. There was no correlation in SLE with presence of CNS disease and either titer or target cell specificity of lymphocytotoxic antibodies. In contrast, patients with multiple sclerosis or meningitis, possessing CSF lymphocytotoxic antibodies, had specificities directed only against T cells, suggesting their origin as autoimmunization with inflamed brain.
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U2 - 10.1016/0090-1229(77)90054-X
DO - 10.1016/0090-1229(77)90054-X
M3 - Article
VL - 7
SP - 269
EP - 280
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 2
ER -