Abstract
Allergic asthma is a complex immunologically mediated disease associated with increased oxidative stress and altered antioxidant defenses. It was hypothesized that α-tocopherol (α-T) decreases oxidative stress and therefore its absence may influence allergic inflammatory process, a pathobiology known to be accompanied by oxidative stress. Therefore, selected parameters of allergic asthma sensitization and inflammation were evaluated following ovalbumin sensitization and re-challenge of α-T transfer protein (TTP) knock-out mice (TTP-/-) that have greatly reduced lung α-T levels (e.g.<5%) compared to their litter mate controls (TTP+/+). Results showed that severe α-T deficiency result in a blunted lung expression of IL-5 mRNA and IL-5 protein and plasma IgE levels compared with TTP+/+ mice following immune sensitization and rechallenge, although lung lavage eosinophil levels were comparable in both genomic strains. It is concluded that the initial stimulation of immune responses by the TTP-/- mice were generally blunted compared to the TTP+/+ mice, thus diminishing some aspects of subsequent allergic inflammatory processes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 387-396 |
| Number of pages | 10 |
| Journal | Free Radical Research |
| Volume | 42 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2008 |
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Keywords
- Allergic inflammation
- Asthma
- Cytokines
- Eosinophils
- IgE
- Vitamin E
ASJC Scopus subject areas
- Biochemistry
Cite this
Severe Vitamin E deficiency modulates airway allergic inflammatory responses in the murine asthma model. / Lim, Yunsook; Vasu, Vihas; Valacchi, Giuseppe; Leonard, Scott; Aung, Hnin Hnin; Schock, Bettina C.; Kenyon, Nicholas; Li, Chin-Shang; Traber, Maret; Cross, Carroll E.
In: Free Radical Research, Vol. 42, No. 4, 2008, p. 387-396.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Severe Vitamin E deficiency modulates airway allergic inflammatory responses in the murine asthma model
AU - Lim, Yunsook
AU - Vasu, Vihas
AU - Valacchi, Giuseppe
AU - Leonard, Scott
AU - Aung, Hnin Hnin
AU - Schock, Bettina C.
AU - Kenyon, Nicholas
AU - Li, Chin-Shang
AU - Traber, Maret
AU - Cross, Carroll E
PY - 2008
Y1 - 2008
N2 - Allergic asthma is a complex immunologically mediated disease associated with increased oxidative stress and altered antioxidant defenses. It was hypothesized that α-tocopherol (α-T) decreases oxidative stress and therefore its absence may influence allergic inflammatory process, a pathobiology known to be accompanied by oxidative stress. Therefore, selected parameters of allergic asthma sensitization and inflammation were evaluated following ovalbumin sensitization and re-challenge of α-T transfer protein (TTP) knock-out mice (TTP-/-) that have greatly reduced lung α-T levels (e.g.<5%) compared to their litter mate controls (TTP+/+). Results showed that severe α-T deficiency result in a blunted lung expression of IL-5 mRNA and IL-5 protein and plasma IgE levels compared with TTP+/+ mice following immune sensitization and rechallenge, although lung lavage eosinophil levels were comparable in both genomic strains. It is concluded that the initial stimulation of immune responses by the TTP-/- mice were generally blunted compared to the TTP+/+ mice, thus diminishing some aspects of subsequent allergic inflammatory processes.
AB - Allergic asthma is a complex immunologically mediated disease associated with increased oxidative stress and altered antioxidant defenses. It was hypothesized that α-tocopherol (α-T) decreases oxidative stress and therefore its absence may influence allergic inflammatory process, a pathobiology known to be accompanied by oxidative stress. Therefore, selected parameters of allergic asthma sensitization and inflammation were evaluated following ovalbumin sensitization and re-challenge of α-T transfer protein (TTP) knock-out mice (TTP-/-) that have greatly reduced lung α-T levels (e.g.<5%) compared to their litter mate controls (TTP+/+). Results showed that severe α-T deficiency result in a blunted lung expression of IL-5 mRNA and IL-5 protein and plasma IgE levels compared with TTP+/+ mice following immune sensitization and rechallenge, although lung lavage eosinophil levels were comparable in both genomic strains. It is concluded that the initial stimulation of immune responses by the TTP-/- mice were generally blunted compared to the TTP+/+ mice, thus diminishing some aspects of subsequent allergic inflammatory processes.
KW - Allergic inflammation
KW - Asthma
KW - Cytokines
KW - Eosinophils
KW - IgE
KW - Vitamin E
UR - http://www.scopus.com/inward/record.url?scp=48849101143&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=48849101143&partnerID=8YFLogxK
U2 - 10.1080/10715760801976600
DO - 10.1080/10715760801976600
M3 - Article
C2 - 18404538
AN - SCOPUS:48849101143
VL - 42
SP - 387
EP - 396
JO - Free Radical Research
JF - Free Radical Research
SN - 1071-5762
IS - 4
ER -