Abstract
Simple chelates and chelate conjugated monoclonal antibodies labeled with 111In, 57Co and 67Cu demonstrate marked differences in stability when exposed to a serum environment. Among these radiometals, on DTPA, the order of stability is 111In 57Co ≫ 67Cu. On benzyl-EDTA, the order of stability is 111In {reversed tilde equals} 57Co ≫ 67Cu. Among those investigated, the only serum stable 67Cu chelate found was 67Cu-TETA. The order of stability observed for 57Co vs 67Cu is contrary to published equilibrium constants. These in vitro studies suggest that the in vivo behavior of metal chelates exposed to a complex molecular environment may not be predicted by classically determined equilibrium constants.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 363-368 |
| Number of pages | 6 |
| Journal | International Journal of Radiation Applications and Instrumentation. |
| Volume | 13 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1986 |
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Serum stability of 67Cu chelates : Comparison with 111In and 57Co. / Cole, William C.; DeNardo, Sally J.; Meares, Claude F.; McCall, Michael J.; DeNardo, Gerald L.; Epstein, Alan L.; O'Brien, Harold A.; Moi, Min K.
In: International Journal of Radiation Applications and Instrumentation., Vol. 13, No. 4, 1986, p. 363-368.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Serum stability of 67Cu chelates
T2 - Comparison with 111In and 57Co
AU - Cole, William C.
AU - DeNardo, Sally J.
AU - Meares, Claude F.
AU - McCall, Michael J.
AU - DeNardo, Gerald L.
AU - Epstein, Alan L.
AU - O'Brien, Harold A.
AU - Moi, Min K.
PY - 1986
Y1 - 1986
N2 - Simple chelates and chelate conjugated monoclonal antibodies labeled with 111In, 57Co and 67Cu demonstrate marked differences in stability when exposed to a serum environment. Among these radiometals, on DTPA, the order of stability is 111In 57Co ≫ 67Cu. On benzyl-EDTA, the order of stability is 111In {reversed tilde equals} 57Co ≫ 67Cu. Among those investigated, the only serum stable 67Cu chelate found was 67Cu-TETA. The order of stability observed for 57Co vs 67Cu is contrary to published equilibrium constants. These in vitro studies suggest that the in vivo behavior of metal chelates exposed to a complex molecular environment may not be predicted by classically determined equilibrium constants.
AB - Simple chelates and chelate conjugated monoclonal antibodies labeled with 111In, 57Co and 67Cu demonstrate marked differences in stability when exposed to a serum environment. Among these radiometals, on DTPA, the order of stability is 111In 57Co ≫ 67Cu. On benzyl-EDTA, the order of stability is 111In {reversed tilde equals} 57Co ≫ 67Cu. Among those investigated, the only serum stable 67Cu chelate found was 67Cu-TETA. The order of stability observed for 57Co vs 67Cu is contrary to published equilibrium constants. These in vitro studies suggest that the in vivo behavior of metal chelates exposed to a complex molecular environment may not be predicted by classically determined equilibrium constants.
UR - http://www.scopus.com/inward/record.url?scp=46149135636&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=46149135636&partnerID=8YFLogxK
U2 - 10.1016/0883-2897(86)90011-5
DO - 10.1016/0883-2897(86)90011-5
M3 - Article
VL - 13
SP - 363
EP - 368
JO - Nuclear Medicine and Biology
JF - Nuclear Medicine and Biology
SN - 0969-8051
IS - 4
ER -