Serum stability of 67Cu chelates: Comparison with 111In and 57Co

William C. Cole, Sally J. DeNardo, Claude F. Meares, Michael J. McCall, Gerald L. DeNardo, Alan L. Epstein, Harold A. O'Brien, Min K. Moi

Research output: Contribution to journalArticle

Abstract

Simple chelates and chelate conjugated monoclonal antibodies labeled with 111In, 57Co and 67Cu demonstrate marked differences in stability when exposed to a serum environment. Among these radiometals, on DTPA, the order of stability is 111In 57Co ≫ 67Cu. On benzyl-EDTA, the order of stability is 111In {reversed tilde equals} 57Co ≫ 67Cu. Among those investigated, the only serum stable 67Cu chelate found was 67Cu-TETA. The order of stability observed for 57Co vs 67Cu is contrary to published equilibrium constants. These in vitro studies suggest that the in vivo behavior of metal chelates exposed to a complex molecular environment may not be predicted by classically determined equilibrium constants.

Original languageEnglish (US)
Pages (from-to)363-368
Number of pages6
JournalInternational Journal of Radiation Applications and Instrumentation.
Volume13
Issue number4
DOIs
StatePublished - 1986

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Pentetic Acid
Serum
Metals
Monoclonal Antibodies
In Vitro Techniques
benzyl-EDTA

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Cole, W. C., DeNardo, S. J., Meares, C. F., McCall, M. J., DeNardo, G. L., Epstein, A. L., ... Moi, M. K. (1986). Serum stability of 67Cu chelates: Comparison with 111In and 57Co. International Journal of Radiation Applications and Instrumentation., 13(4), 363-368. https://doi.org/10.1016/0883-2897(86)90011-5

Serum stability of 67Cu chelates : Comparison with 111In and 57Co. / Cole, William C.; DeNardo, Sally J.; Meares, Claude F.; McCall, Michael J.; DeNardo, Gerald L.; Epstein, Alan L.; O'Brien, Harold A.; Moi, Min K.

In: International Journal of Radiation Applications and Instrumentation., Vol. 13, No. 4, 1986, p. 363-368.

Research output: Contribution to journalArticle

Cole, WC, DeNardo, SJ, Meares, CF, McCall, MJ, DeNardo, GL, Epstein, AL, O'Brien, HA & Moi, MK 1986, 'Serum stability of 67Cu chelates: Comparison with 111In and 57Co', International Journal of Radiation Applications and Instrumentation., vol. 13, no. 4, pp. 363-368. https://doi.org/10.1016/0883-2897(86)90011-5
Cole, William C. ; DeNardo, Sally J. ; Meares, Claude F. ; McCall, Michael J. ; DeNardo, Gerald L. ; Epstein, Alan L. ; O'Brien, Harold A. ; Moi, Min K. / Serum stability of 67Cu chelates : Comparison with 111In and 57Co. In: International Journal of Radiation Applications and Instrumentation. 1986 ; Vol. 13, No. 4. pp. 363-368.
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abstract = "Simple chelates and chelate conjugated monoclonal antibodies labeled with 111In, 57Co and 67Cu demonstrate marked differences in stability when exposed to a serum environment. Among these radiometals, on DTPA, the order of stability is 111In 57Co ≫ 67Cu. On benzyl-EDTA, the order of stability is 111In {reversed tilde equals} 57Co ≫ 67Cu. Among those investigated, the only serum stable 67Cu chelate found was 67Cu-TETA. The order of stability observed for 57Co vs 67Cu is contrary to published equilibrium constants. These in vitro studies suggest that the in vivo behavior of metal chelates exposed to a complex molecular environment may not be predicted by classically determined equilibrium constants.",
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AB - Simple chelates and chelate conjugated monoclonal antibodies labeled with 111In, 57Co and 67Cu demonstrate marked differences in stability when exposed to a serum environment. Among these radiometals, on DTPA, the order of stability is 111In 57Co ≫ 67Cu. On benzyl-EDTA, the order of stability is 111In {reversed tilde equals} 57Co ≫ 67Cu. Among those investigated, the only serum stable 67Cu chelate found was 67Cu-TETA. The order of stability observed for 57Co vs 67Cu is contrary to published equilibrium constants. These in vitro studies suggest that the in vivo behavior of metal chelates exposed to a complex molecular environment may not be predicted by classically determined equilibrium constants.

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