Serum resistance as an indicator of virulence of Pasteurella multocida for turkeys.

T. Y. Morishita, K. P. Snipes, Tim Carpenter

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Wildlife isolates of Pasteurella multocida, whose virulence for turkeys had previously been determined by intravenous inoculation, were characterized regarding their ability to survive incubation in fresh non-immune turkey serum. The relative virulence of the isolates was significantly associated with their ability to resist the bactericidal power of the serum as determined by standard plate counts following incubation. Organisms with a high survival value were more virulent; those with a low survival value were less virulent. A statistical model was specified and was successfully used to predict relative virulence of the P. multocida isolates. This method of assaying serum resistance was rapid, repeatable, and practical and could be performed with minimal laboratory equipment. Also studied was the serum resistance of seven serotype 3, 4 isolates obtained from the lungs of M9-vaccinated turkeys from seven flocks experiencing increased mortality due to fowl cholera. These isolates were shown to be identical to the M9 vaccine by restriction endonuclease analysis of chromosomal DNA. Six of the seven isolates had higher serum survival values than the original M9 vaccine.

Original languageEnglish (US)
Pages (from-to)888-892
Number of pages5
JournalAvian Diseases
Volume34
Issue number4
StatePublished - Oct 1990

Fingerprint

Pasteurella multocida
Virulence
virulence
Serum
laboratory equipment
vaccines
fowl cholera
restriction mapping
statistical models
Vaccines
plate count
wildlife
flocks
serotypes
lungs
vaccination
Cholera
DNA Restriction Enzymes
Statistical Models
organisms

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • veterinary(all)

Cite this

Serum resistance as an indicator of virulence of Pasteurella multocida for turkeys. / Morishita, T. Y.; Snipes, K. P.; Carpenter, Tim.

In: Avian Diseases, Vol. 34, No. 4, 10.1990, p. 888-892.

Research output: Contribution to journalArticle

Morishita, T. Y. ; Snipes, K. P. ; Carpenter, Tim. / Serum resistance as an indicator of virulence of Pasteurella multocida for turkeys. In: Avian Diseases. 1990 ; Vol. 34, No. 4. pp. 888-892.
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