Serum protein profile alterations in hemodialysis patients

Richard G. Langlois, James E. Trebes, Enrique A. Dalmasso, Yong Ying, Robert W. Davies, Mario P. Curzi, Bill W. Colston, Ken W Turteltaub, Julie Perkins, Brett A. Chromy, Megan W. Choi, Gloria A. Murphy, J. Patrick Fitch, Sandra L. McCutchen-Maloney

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Background: Serum protein profiling patterns can reflect the pathological state of a patient and therefore may be useful for clinical diagnostics. Here, we present results from a pilot study of proteomic expression patterns in hemodialysis patients designed to evaluate the range of serum proteomic alterations in this population. Methods: Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was used to analyze serum obtained from patients on periodic hemodialysis treatment and healthy controls. Serum samples from patients and controls were first fractionated into six eluants on a strong anion exchange column, followed by application to four array chemistries representing cation exchange, anion exchange, metal affinity and hydrophobic surfaces. A total of 144 SELDI-TOF-MS spectra were obtained from each serum sample. Results: The overall profiles of the patient and control samples were consistent and reproducible. However, 30 well-defined protein differences were observed; 15 proteins were elevated and 15 were decreased in patients compared to controls. Serum from 1 patient exhibited novel protein peaks suggesting possible additional changes due to a secondary disease process. Conclusion: SELDI-TOF-MS demonstrated consistent serum protein profile differences between patients and controls. Similarity in protein profiles among dialysis patients suggests that patient physiological responses to end-stage renal disease and/or dialysis therapy have a major effect on serum protein profiles.

Original languageEnglish (US)
Pages (from-to)268-274
Number of pages7
JournalAmerican Journal of Nephrology
Volume24
Issue number2
DOIs
StatePublished - 2004
Externally publishedYes

Keywords

  • Biomarkers
  • Hemodialysis
  • Protein profiling
  • Proteomics
  • SELDI-TOF-MS

ASJC Scopus subject areas

  • Nephrology

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