Serum protein profile alterations in hemodialysis patients

Richard G. Langlois, James E. Trebes, Enrique A. Dalmasso, Yong Ying, Robert W. Davies, Mario P. Curzi, Bill W. Colston, Ken W Turteltaub, Julie Perkins, Brett A. Chromy, Megan W. Choi, Gloria A. Murphy, J. Patrick Fitch, Sandra L. McCutchen-Maloney

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Background: Serum protein profiling patterns can reflect the pathological state of a patient and therefore may be useful for clinical diagnostics. Here, we present results from a pilot study of proteomic expression patterns in hemodialysis patients designed to evaluate the range of serum proteomic alterations in this population. Methods: Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was used to analyze serum obtained from patients on periodic hemodialysis treatment and healthy controls. Serum samples from patients and controls were first fractionated into six eluants on a strong anion exchange column, followed by application to four array chemistries representing cation exchange, anion exchange, metal affinity and hydrophobic surfaces. A total of 144 SELDI-TOF-MS spectra were obtained from each serum sample. Results: The overall profiles of the patient and control samples were consistent and reproducible. However, 30 well-defined protein differences were observed; 15 proteins were elevated and 15 were decreased in patients compared to controls. Serum from 1 patient exhibited novel protein peaks suggesting possible additional changes due to a secondary disease process. Conclusion: SELDI-TOF-MS demonstrated consistent serum protein profile differences between patients and controls. Similarity in protein profiles among dialysis patients suggests that patient physiological responses to end-stage renal disease and/or dialysis therapy have a major effect on serum protein profiles.

Original languageEnglish (US)
Pages (from-to)268-274
Number of pages7
JournalAmerican Journal of Nephrology
Volume24
Issue number2
DOIs
StatePublished - 2004
Externally publishedYes

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Keywords

  • Biomarkers
  • Hemodialysis
  • Protein profiling
  • Proteomics
  • SELDI-TOF-MS

ASJC Scopus subject areas

  • Nephrology

Cite this

Langlois, R. G., Trebes, J. E., Dalmasso, E. A., Ying, Y., Davies, R. W., Curzi, M. P., Colston, B. W., Turteltaub, K. W., Perkins, J., Chromy, B. A., Choi, M. W., Murphy, G. A., Fitch, J. P., & McCutchen-Maloney, S. L. (2004). Serum protein profile alterations in hemodialysis patients. American Journal of Nephrology, 24(2), 268-274. https://doi.org/10.1159/000077409