Serum protein profile alterations in hemodialysis patients

Richard G. Langlois, James E. Trebes, Enrique A. Dalmasso, Yong Ying, Robert W. Davies, Mario P. Curzi, Bill W. Colston, Ken W Turteltaub, Julie Perkins, Brett A. Chromy, Megan W. Choi, Gloria A. Murphy, J. Patrick Fitch, Sandra L. McCutchen-Maloney

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Serum protein profiling patterns can reflect the pathological state of a patient and therefore may be useful for clinical diagnostics. Here, we present results from a pilot study of proteomic expression patterns in hemodialysis patients designed to evaluate the range of serum proteomic alterations in this population. Methods: Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was used to analyze serum obtained from patients on periodic hemodialysis treatment and healthy controls. Serum samples from patients and controls were first fractionated into six eluants on a strong anion exchange column, followed by application to four array chemistries representing cation exchange, anion exchange, metal affinity and hydrophobic surfaces. A total of 144 SELDI-TOF-MS spectra were obtained from each serum sample. Results: The overall profiles of the patient and control samples were consistent and reproducible. However, 30 well-defined protein differences were observed; 15 proteins were elevated and 15 were decreased in patients compared to controls. Serum from 1 patient exhibited novel protein peaks suggesting possible additional changes due to a secondary disease process. Conclusion: SELDI-TOF-MS demonstrated consistent serum protein profile differences between patients and controls. Similarity in protein profiles among dialysis patients suggests that patient physiological responses to end-stage renal disease and/or dialysis therapy have a major effect on serum protein profiles.

Original languageEnglish (US)
Pages (from-to)268-274
Number of pages7
JournalAmerican Journal of Nephrology
Volume24
Issue number2
DOIs
StatePublished - 2004
Externally publishedYes

Fingerprint

Renal Dialysis
Blood Proteins
Mass Spectrometry
Serum
Lasers
Proteomics
Anions
Dialysis
Proteins
Chronic Kidney Failure
Cations
Metals
Therapeutics
Population

Keywords

  • Biomarkers
  • Hemodialysis
  • Protein profiling
  • Proteomics
  • SELDI-TOF-MS

ASJC Scopus subject areas

  • Nephrology

Cite this

Langlois, R. G., Trebes, J. E., Dalmasso, E. A., Ying, Y., Davies, R. W., Curzi, M. P., ... McCutchen-Maloney, S. L. (2004). Serum protein profile alterations in hemodialysis patients. American Journal of Nephrology, 24(2), 268-274. https://doi.org/10.1159/000077409

Serum protein profile alterations in hemodialysis patients. / Langlois, Richard G.; Trebes, James E.; Dalmasso, Enrique A.; Ying, Yong; Davies, Robert W.; Curzi, Mario P.; Colston, Bill W.; Turteltaub, Ken W; Perkins, Julie; Chromy, Brett A.; Choi, Megan W.; Murphy, Gloria A.; Fitch, J. Patrick; McCutchen-Maloney, Sandra L.

In: American Journal of Nephrology, Vol. 24, No. 2, 2004, p. 268-274.

Research output: Contribution to journalArticle

Langlois, RG, Trebes, JE, Dalmasso, EA, Ying, Y, Davies, RW, Curzi, MP, Colston, BW, Turteltaub, KW, Perkins, J, Chromy, BA, Choi, MW, Murphy, GA, Fitch, JP & McCutchen-Maloney, SL 2004, 'Serum protein profile alterations in hemodialysis patients', American Journal of Nephrology, vol. 24, no. 2, pp. 268-274. https://doi.org/10.1159/000077409
Langlois RG, Trebes JE, Dalmasso EA, Ying Y, Davies RW, Curzi MP et al. Serum protein profile alterations in hemodialysis patients. American Journal of Nephrology. 2004;24(2):268-274. https://doi.org/10.1159/000077409
Langlois, Richard G. ; Trebes, James E. ; Dalmasso, Enrique A. ; Ying, Yong ; Davies, Robert W. ; Curzi, Mario P. ; Colston, Bill W. ; Turteltaub, Ken W ; Perkins, Julie ; Chromy, Brett A. ; Choi, Megan W. ; Murphy, Gloria A. ; Fitch, J. Patrick ; McCutchen-Maloney, Sandra L. / Serum protein profile alterations in hemodialysis patients. In: American Journal of Nephrology. 2004 ; Vol. 24, No. 2. pp. 268-274.
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