Serum-mediated resolution of Lyme arthritis in mice

Stephen W Barthold, Mark DeSouza, Sunlian Feng

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Arthritis and carditis were mildly improved upon adoptive transfer of T cell enriched lymphocyte populations from Borrelia burgdorferi-infected (B. burgdorferi) (immune) compared with naive immunocompetent mice into B. burgdorferi-infected, severe combined immunodeficient (SCID) mice. Despite the relative purity of T cells in the transferred cells, recipient mice seroconverted to B. burgdorferi. Thus, the effect could not be attributed to T cells alone. Passive transfer of serum from actively infected immunocompetent mice (immune serum) to SCID mice at the time of or before B. burgdorferi inoculation, or on Days 4, 8, and 12 after inoculation prevented or cured (respectively) infection and disease when examined at 15 days. Transfer of immune serum on Days 12, 16, 20, 24, and 28 did not clear infection at Day 30 but resulted in resolution of arthritis, indicating that immune serum can cause resolution of joint disease. Immune serum treatment could maintain arthritis resolution for up to 60 days. Immune serum from mice infected for 90 days or 15 months both had strong protective, post- infection, and arthritis-modulating activity, whereas hyperimmune serum to heat-killed B. burgdorferi or recombinant outer surface protein (Osp) A protected mice against infection when given on Day 0-but not at later intervals-and did not modulate disease. Immune serum from 90-day infected mice labeled spirochetes in joint tissues of SCID mice by immunohistochemistry, but hyperimmune serum to heat-killed B. burgdorferi or OspA did not. These studies suggest that the biologically active properties of immune serum may be directed toward yet to be defined, in vivo-expressed antigens of B. burgdorferi.

Original languageEnglish (US)
Pages (from-to)57-67
Number of pages11
JournalLaboratory Investigation
Volume74
Issue number1
StatePublished - Jan 1996
Externally publishedYes

Fingerprint

Borrelia burgdorferi
Lyme Disease
Immune Sera
Arthritis
SCID Mice
Serum
Infection
T-Lymphocytes
Hot Temperature
Spirochaetales
Adoptive Transfer
Joint Diseases
Myocarditis
Joints
Immunohistochemistry
Lymphocytes
Antigens
Population

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Serum-mediated resolution of Lyme arthritis in mice. / Barthold, Stephen W; DeSouza, Mark; Feng, Sunlian.

In: Laboratory Investigation, Vol. 74, No. 1, 01.1996, p. 57-67.

Research output: Contribution to journalArticle

Barthold, SW, DeSouza, M & Feng, S 1996, 'Serum-mediated resolution of Lyme arthritis in mice', Laboratory Investigation, vol. 74, no. 1, pp. 57-67.
Barthold, Stephen W ; DeSouza, Mark ; Feng, Sunlian. / Serum-mediated resolution of Lyme arthritis in mice. In: Laboratory Investigation. 1996 ; Vol. 74, No. 1. pp. 57-67.
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