Serum Inhibin Concentration in Dogs with Adrenal Gland Disease and in Healthy Dogs

C. Brömel, Richard W Nelson, Edward C Feldman, C. J. Munro, Philip H Kass, A. E. Vico, P. Labelle, Alan J Conley

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Studies in humans identified the synthesis and secretion of inhibin from adrenocortical tumors, but not pheochromocytoma (PHEO). Inhibin has not been examined in dogs as a serum biomarker for adrenal gland tumors. Objective: To determine serum inhibin concentration in dogs with adrenal gland disease and in healthy dogs. Animals: Forty-eight neutered dogs with adrenal disease including pituitary-dependent hyperadrenocorticism (PDH, 17), adrenocortical tumor (18), and PHEO (13), and 41 healthy intact or neutered dogs. Methods: Prospective observational study. Dogs were diagnosed with PDH, adrenocortical tumor (hyperadrenocorticism or noncortisol secreting), or PHEO based on clinical signs, endocrine function tests, abdominal ultrasound examination, and histopathology. Inhibin concentration was measured by radioimmunoassay in serum before and after ACTH stimulation, and before and after treatment. Results: In neutered dogs, median inhibin concentration was significantly higher in dogs with adrenocortical tumors (0.82 ng/mL) and PDH (0.16 ng/mL) than in dogs with PHEO and healthy dogs (both undetectable). Median inhibin concentration was significantly higher in dogs with adrenocortical tumors than in those with PDH and decreased after adrenalectomy. Median inhibin concentration was significantly higher in intact than in neutered healthy dogs and was similar in pre- and post-ACTH stimulation. Sensitivity, specificity, and accuracy of serum inhibin concentration for identifying an adrenal tumor as a PHEO were 100, 88.9, and 93.6%, respectively. Conclusions and Clinical Importance: Adrenocortical tumors and PDH but not PHEOs are associated with increased serum inhibin concentration; undetectable inhibin is highly supportive of PHEO in neutered dogs with adrenal tumors.

Original languageEnglish (US)
Pages (from-to)76-82
Number of pages7
JournalJournal of Veterinary Internal Medicine
Volume27
Issue number1
DOIs
StatePublished - Jan 2013

Fingerprint

Adrenal Gland Diseases
adrenal gland diseases
inhibin
Inhibins
Dogs
dogs
Serum
Pheochromocytoma
neoplasms
corticotropin
Neoplasms
Adrenocortical Hyperfunction
Adrenocorticotropic Hormone
hyperadrenocorticism
Glandular and Epithelial Neoplasms
Pituitary Diseases

Keywords

  • Hyperadrenocorticism
  • Neoplasm
  • Pheochromocytoma
  • Pituitary gland
  • Tumor

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Serum Inhibin Concentration in Dogs with Adrenal Gland Disease and in Healthy Dogs. / Brömel, C.; Nelson, Richard W; Feldman, Edward C; Munro, C. J.; Kass, Philip H; Vico, A. E.; Labelle, P.; Conley, Alan J.

In: Journal of Veterinary Internal Medicine, Vol. 27, No. 1, 01.2013, p. 76-82.

Research output: Contribution to journalArticle

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abstract = "Background: Studies in humans identified the synthesis and secretion of inhibin from adrenocortical tumors, but not pheochromocytoma (PHEO). Inhibin has not been examined in dogs as a serum biomarker for adrenal gland tumors. Objective: To determine serum inhibin concentration in dogs with adrenal gland disease and in healthy dogs. Animals: Forty-eight neutered dogs with adrenal disease including pituitary-dependent hyperadrenocorticism (PDH, 17), adrenocortical tumor (18), and PHEO (13), and 41 healthy intact or neutered dogs. Methods: Prospective observational study. Dogs were diagnosed with PDH, adrenocortical tumor (hyperadrenocorticism or noncortisol secreting), or PHEO based on clinical signs, endocrine function tests, abdominal ultrasound examination, and histopathology. Inhibin concentration was measured by radioimmunoassay in serum before and after ACTH stimulation, and before and after treatment. Results: In neutered dogs, median inhibin concentration was significantly higher in dogs with adrenocortical tumors (0.82 ng/mL) and PDH (0.16 ng/mL) than in dogs with PHEO and healthy dogs (both undetectable). Median inhibin concentration was significantly higher in dogs with adrenocortical tumors than in those with PDH and decreased after adrenalectomy. Median inhibin concentration was significantly higher in intact than in neutered healthy dogs and was similar in pre- and post-ACTH stimulation. Sensitivity, specificity, and accuracy of serum inhibin concentration for identifying an adrenal tumor as a PHEO were 100, 88.9, and 93.6{\%}, respectively. Conclusions and Clinical Importance: Adrenocortical tumors and PDH but not PHEOs are associated with increased serum inhibin concentration; undetectable inhibin is highly supportive of PHEO in neutered dogs with adrenal tumors.",
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