Abstract
Background: To perform a comprehensive serum growth factor analysis in dry eye syndrome patients and to compare this with matched controls. Methods: Six female dry eye syndrome patients and six age- and gender-matched controls were recruited. Whole blood was collected, allowed to clot and then centrifuged. Serum was extracted by using sterile technique. Enzyme-linked immunosorbent assays were performed to quantify serum growth factor levels. Results: Levels of transforming growth factor-beta 1 and 2 (TGF-β1 and β2), nerve growth factor (NGF), insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF), acidic and basic fibroblast growth factor (FGF), keratinocyte growth factor (KGF), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), platelet-derived growth factor-AA, AB and BB (PDGF-AA, AB and BB), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and glial cell line-derived neurotrophic factor (GDNF) were quantified, and statistical analysis was performed by using the Mann-Whitney U-test with the Bonferroni correction. Conclusions: No significant difference was found between serum growth factor levels in dry eye syndrome patients versus controls. Our study provides comprehensive analysis of serum growth factor levels in autologous serum eye drops produced from ocular surface disease patients. A knowledge of growth factor levels in serum may be important because of the increasing use of autologous serum eye drops in refractory ocular surface diseases and for an understanding of how topical serum may provide benefit.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 717-720 |
| Number of pages | 4 |
| Journal | Clinical and Experimental Ophthalmology |
| Volume | 36 |
| Issue number | 8 |
| DOIs | |
| State | Published - 2008 |
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Keywords
- Anterior segment
- Autologous serum
- Dry eye syndrome
- Growth factor
ASJC Scopus subject areas
- Ophthalmology
Cite this
Serum growth factor analysis in dry eye syndrome. / Bradley, Jay C.; Bradley, Rachael H.; Mccartney, David L.; Mannis, Mark J.
In: Clinical and Experimental Ophthalmology, Vol. 36, No. 8, 2008, p. 717-720.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Serum growth factor analysis in dry eye syndrome
AU - Bradley, Jay C.
AU - Bradley, Rachael H.
AU - Mccartney, David L.
AU - Mannis, Mark J
PY - 2008
Y1 - 2008
N2 - Background: To perform a comprehensive serum growth factor analysis in dry eye syndrome patients and to compare this with matched controls. Methods: Six female dry eye syndrome patients and six age- and gender-matched controls were recruited. Whole blood was collected, allowed to clot and then centrifuged. Serum was extracted by using sterile technique. Enzyme-linked immunosorbent assays were performed to quantify serum growth factor levels. Results: Levels of transforming growth factor-beta 1 and 2 (TGF-β1 and β2), nerve growth factor (NGF), insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF), acidic and basic fibroblast growth factor (FGF), keratinocyte growth factor (KGF), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), platelet-derived growth factor-AA, AB and BB (PDGF-AA, AB and BB), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and glial cell line-derived neurotrophic factor (GDNF) were quantified, and statistical analysis was performed by using the Mann-Whitney U-test with the Bonferroni correction. Conclusions: No significant difference was found between serum growth factor levels in dry eye syndrome patients versus controls. Our study provides comprehensive analysis of serum growth factor levels in autologous serum eye drops produced from ocular surface disease patients. A knowledge of growth factor levels in serum may be important because of the increasing use of autologous serum eye drops in refractory ocular surface diseases and for an understanding of how topical serum may provide benefit.
AB - Background: To perform a comprehensive serum growth factor analysis in dry eye syndrome patients and to compare this with matched controls. Methods: Six female dry eye syndrome patients and six age- and gender-matched controls were recruited. Whole blood was collected, allowed to clot and then centrifuged. Serum was extracted by using sterile technique. Enzyme-linked immunosorbent assays were performed to quantify serum growth factor levels. Results: Levels of transforming growth factor-beta 1 and 2 (TGF-β1 and β2), nerve growth factor (NGF), insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF), acidic and basic fibroblast growth factor (FGF), keratinocyte growth factor (KGF), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), platelet-derived growth factor-AA, AB and BB (PDGF-AA, AB and BB), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and glial cell line-derived neurotrophic factor (GDNF) were quantified, and statistical analysis was performed by using the Mann-Whitney U-test with the Bonferroni correction. Conclusions: No significant difference was found between serum growth factor levels in dry eye syndrome patients versus controls. Our study provides comprehensive analysis of serum growth factor levels in autologous serum eye drops produced from ocular surface disease patients. A knowledge of growth factor levels in serum may be important because of the increasing use of autologous serum eye drops in refractory ocular surface diseases and for an understanding of how topical serum may provide benefit.
KW - Anterior segment
KW - Autologous serum
KW - Dry eye syndrome
KW - Growth factor
UR - http://www.scopus.com/inward/record.url?scp=58149359046&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149359046&partnerID=8YFLogxK
U2 - 10.1111/j.1442-9071.2008.01895.x
DO - 10.1111/j.1442-9071.2008.01895.x
M3 - Article
C2 - 19128374
AN - SCOPUS:58149359046
VL - 36
SP - 717
EP - 720
JO - Clinical and Experimental Ophthalmology
JF - Clinical and Experimental Ophthalmology
SN - 1442-6404
IS - 8
ER -