Serum glycosylation characterization of osteonecrosis of the femoral head by mass spectrometry

Ting Song, Peng Chen, Carol Stroble, L. Renee Ruhaak, Haibin Wang, Ziqi Li, Wei He, Carlito B Lebrilla

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Osteonecrosis of the femoral head is a recalcitrant and paralyzing disease often discovered in the end stage at the time of diagnosis, which is often performed by physical examination and diagnostic imaging. Osteonecrosis of the femoral head is typically caused by trauma or long-term steroid use. There are over 30 million patients in the US taking steroids, and roughly 40% will develop osteonecrosis of the femoral head. However, the exact pathophysiological process is not well understood. This study aims to examine the alteration in serum glycosylation of osteonecrosis of the femoral head using the state-of-the-art analytical tools to provide more chemical data for pathophysiology research and possibly biomarker discovery. A training set containing 27 serum samples from steroid-induced osteonecrosis of the femoral head patients and 25 from gender- and age-matched controls was collected and analyzed. Glycosylation of whole serum and site-specific glycosylation of immunoglobulins are characterized using electrospray ionization-Q-time of flight and electrospray ionization-Triple-Quadruple via multiple reaction monitoring, respectively. The whole serum glycosylation analysis yielded 14 N-glycan compositions and multiple reaction monitoring yielded eight glycopeptides that were altered between cases and controls with statistical significance. The increase of nonsialylated, nonfucosylated N-glycans and decrease of fucosylated N-glycans are associated with the development of osteonecrosis of the femoral head. Glycosylation is a posttranslational protein modification and is apparently affected by osteonecrosis of the femoral head. Future studies with a larger cohort and patients from earlier stage will be performed to assess these potential markers’ value in disease onset.

Original languageEnglish (US)
Pages (from-to)178-187
Number of pages10
JournalEuropean Journal of Mass Spectrometry
Volume24
Issue number1
DOIs
StatePublished - Feb 1 2018

Fingerprint

Glycosylation
serums
steroids
Mass spectrometry
mass spectroscopy
Polysaccharides
Electrospray ionization
Steroids
physical examinations
ionization
biomarkers
markers
Glycopeptides
Monitoring
Biomarkers
education
Immunoglobulins
proteins
Imaging techniques
Chemical analysis

Keywords

  • Biomarker
  • liquid chromatography
  • mass spectrometry
  • N-glycan
  • osteonecrosis of the femoral head

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Spectroscopy

Cite this

Song, T., Chen, P., Stroble, C., Ruhaak, L. R., Wang, H., Li, Z., ... Lebrilla, C. B. (2018). Serum glycosylation characterization of osteonecrosis of the femoral head by mass spectrometry. European Journal of Mass Spectrometry, 24(1), 178-187. https://doi.org/10.1177/1469066717740010

Serum glycosylation characterization of osteonecrosis of the femoral head by mass spectrometry. / Song, Ting; Chen, Peng; Stroble, Carol; Ruhaak, L. Renee; Wang, Haibin; Li, Ziqi; He, Wei; Lebrilla, Carlito B.

In: European Journal of Mass Spectrometry, Vol. 24, No. 1, 01.02.2018, p. 178-187.

Research output: Contribution to journalArticle

Song, T, Chen, P, Stroble, C, Ruhaak, LR, Wang, H, Li, Z, He, W & Lebrilla, CB 2018, 'Serum glycosylation characterization of osteonecrosis of the femoral head by mass spectrometry', European Journal of Mass Spectrometry, vol. 24, no. 1, pp. 178-187. https://doi.org/10.1177/1469066717740010
Song, Ting ; Chen, Peng ; Stroble, Carol ; Ruhaak, L. Renee ; Wang, Haibin ; Li, Ziqi ; He, Wei ; Lebrilla, Carlito B. / Serum glycosylation characterization of osteonecrosis of the femoral head by mass spectrometry. In: European Journal of Mass Spectrometry. 2018 ; Vol. 24, No. 1. pp. 178-187.
@article{22fc6f0605df476fb228d7cf5214ed12,
title = "Serum glycosylation characterization of osteonecrosis of the femoral head by mass spectrometry",
abstract = "Osteonecrosis of the femoral head is a recalcitrant and paralyzing disease often discovered in the end stage at the time of diagnosis, which is often performed by physical examination and diagnostic imaging. Osteonecrosis of the femoral head is typically caused by trauma or long-term steroid use. There are over 30 million patients in the US taking steroids, and roughly 40{\%} will develop osteonecrosis of the femoral head. However, the exact pathophysiological process is not well understood. This study aims to examine the alteration in serum glycosylation of osteonecrosis of the femoral head using the state-of-the-art analytical tools to provide more chemical data for pathophysiology research and possibly biomarker discovery. A training set containing 27 serum samples from steroid-induced osteonecrosis of the femoral head patients and 25 from gender- and age-matched controls was collected and analyzed. Glycosylation of whole serum and site-specific glycosylation of immunoglobulins are characterized using electrospray ionization-Q-time of flight and electrospray ionization-Triple-Quadruple via multiple reaction monitoring, respectively. The whole serum glycosylation analysis yielded 14 N-glycan compositions and multiple reaction monitoring yielded eight glycopeptides that were altered between cases and controls with statistical significance. The increase of nonsialylated, nonfucosylated N-glycans and decrease of fucosylated N-glycans are associated with the development of osteonecrosis of the femoral head. Glycosylation is a posttranslational protein modification and is apparently affected by osteonecrosis of the femoral head. Future studies with a larger cohort and patients from earlier stage will be performed to assess these potential markers’ value in disease onset.",
keywords = "Biomarker, liquid chromatography, mass spectrometry, N-glycan, osteonecrosis of the femoral head",
author = "Ting Song and Peng Chen and Carol Stroble and Ruhaak, {L. Renee} and Haibin Wang and Ziqi Li and Wei He and Lebrilla, {Carlito B}",
year = "2018",
month = "2",
day = "1",
doi = "10.1177/1469066717740010",
language = "English (US)",
volume = "24",
pages = "178--187",
journal = "European Journal of Mass Spectrometry",
issn = "1469-0667",
publisher = "I M Publications",
number = "1",

}

TY - JOUR

T1 - Serum glycosylation characterization of osteonecrosis of the femoral head by mass spectrometry

AU - Song, Ting

AU - Chen, Peng

AU - Stroble, Carol

AU - Ruhaak, L. Renee

AU - Wang, Haibin

AU - Li, Ziqi

AU - He, Wei

AU - Lebrilla, Carlito B

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Osteonecrosis of the femoral head is a recalcitrant and paralyzing disease often discovered in the end stage at the time of diagnosis, which is often performed by physical examination and diagnostic imaging. Osteonecrosis of the femoral head is typically caused by trauma or long-term steroid use. There are over 30 million patients in the US taking steroids, and roughly 40% will develop osteonecrosis of the femoral head. However, the exact pathophysiological process is not well understood. This study aims to examine the alteration in serum glycosylation of osteonecrosis of the femoral head using the state-of-the-art analytical tools to provide more chemical data for pathophysiology research and possibly biomarker discovery. A training set containing 27 serum samples from steroid-induced osteonecrosis of the femoral head patients and 25 from gender- and age-matched controls was collected and analyzed. Glycosylation of whole serum and site-specific glycosylation of immunoglobulins are characterized using electrospray ionization-Q-time of flight and electrospray ionization-Triple-Quadruple via multiple reaction monitoring, respectively. The whole serum glycosylation analysis yielded 14 N-glycan compositions and multiple reaction monitoring yielded eight glycopeptides that were altered between cases and controls with statistical significance. The increase of nonsialylated, nonfucosylated N-glycans and decrease of fucosylated N-glycans are associated with the development of osteonecrosis of the femoral head. Glycosylation is a posttranslational protein modification and is apparently affected by osteonecrosis of the femoral head. Future studies with a larger cohort and patients from earlier stage will be performed to assess these potential markers’ value in disease onset.

AB - Osteonecrosis of the femoral head is a recalcitrant and paralyzing disease often discovered in the end stage at the time of diagnosis, which is often performed by physical examination and diagnostic imaging. Osteonecrosis of the femoral head is typically caused by trauma or long-term steroid use. There are over 30 million patients in the US taking steroids, and roughly 40% will develop osteonecrosis of the femoral head. However, the exact pathophysiological process is not well understood. This study aims to examine the alteration in serum glycosylation of osteonecrosis of the femoral head using the state-of-the-art analytical tools to provide more chemical data for pathophysiology research and possibly biomarker discovery. A training set containing 27 serum samples from steroid-induced osteonecrosis of the femoral head patients and 25 from gender- and age-matched controls was collected and analyzed. Glycosylation of whole serum and site-specific glycosylation of immunoglobulins are characterized using electrospray ionization-Q-time of flight and electrospray ionization-Triple-Quadruple via multiple reaction monitoring, respectively. The whole serum glycosylation analysis yielded 14 N-glycan compositions and multiple reaction monitoring yielded eight glycopeptides that were altered between cases and controls with statistical significance. The increase of nonsialylated, nonfucosylated N-glycans and decrease of fucosylated N-glycans are associated with the development of osteonecrosis of the femoral head. Glycosylation is a posttranslational protein modification and is apparently affected by osteonecrosis of the femoral head. Future studies with a larger cohort and patients from earlier stage will be performed to assess these potential markers’ value in disease onset.

KW - Biomarker

KW - liquid chromatography

KW - mass spectrometry

KW - N-glycan

KW - osteonecrosis of the femoral head

UR - http://www.scopus.com/inward/record.url?scp=85041024833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041024833&partnerID=8YFLogxK

U2 - 10.1177/1469066717740010

DO - 10.1177/1469066717740010

M3 - Article

C2 - 29224385

AN - SCOPUS:85041024833

VL - 24

SP - 178

EP - 187

JO - European Journal of Mass Spectrometry

JF - European Journal of Mass Spectrometry

SN - 1469-0667

IS - 1

ER -