Serum concentrations of oestradiol-17β, progesterone, relaxin and chorionic gonadotrophin during blastocyst implantation in natural pregnancy cycle and in embryo transfer cycle in the rhesus monkey

Debabrata Ghosh, Dennis R. Stewart, Nihar Ranjan Nayak, Bill L. Lasley, James W. Overstreet, Andrew G Hendrickx, Jayasree Sengupta

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The present study was undertaken to assess the temporal association between the profiles of serum concentrations of oestradiol-17β, progesterone, chorionic gonadotrophin (CG) and relaxin in pregnancies established naturally, and after embryo transfer, as well as in failed pregnancies in rhesus monkeys. In naturally mated cycles (group 1) a conception rate of 75% was obtained. In group 1, the mean day of CG detection in serum was 11.5 ± 1.9 day post-ovulation, and for relaxin, 9.0 ± 2.5 day post-ovulation. In group 2, embryo transfer to synchronous, non-mated surrogate recipients was performed; seven embryo transfer cycles yielded three pregnancies which were allowed to continue to term and normal infants were delivered. In embryo transfer cycles the mean day of CG detection was 14.8 ± 1.8 day post-ovulation, and for relaxin, 11.4 ± 2.6 day post-ovulation. A delay of about 3 days was observed in the appearance in circulation of CG (P < 0.05) and also of relaxin (P < 0.05) between natural mated and embryo transfer conception cycles. Significant differences (P < 0.05 for progesterone and P < 0.03 for oestradiol) were obtained for the areas under the curves for progesterone and oestradiol between days 12 and 16 in conception cycles compared with failed pregnancies. These data provide the first observation of the normal hormonal signals associated with maternal recognition of transferred embryos during the peri-implantation period, and suggest that the use of such an experimental primate embryo transfer model may help to elucidate components of maternal and embryonic signal-response mechanisms during embryo implantation.

Original languageEnglish (US)
Pages (from-to)914-920
Number of pages7
JournalHuman Reproduction
Volume12
Issue number5
StatePublished - 1997

Fingerprint

Relaxin
Embryo Transfer
Chorionic Gonadotropin
Macaca mulatta
Progesterone
Estradiol
Ovulation
Pregnancy
Serum
Mothers
Primates
Area Under Curve
Embryonic Structures
Observation

Keywords

  • Chorionic gonadotrophin
  • Oestradiol-17β
  • Progesterone
  • Relaxin
  • Rhesus monkey

ASJC Scopus subject areas

  • Developmental Biology
  • Physiology
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Serum concentrations of oestradiol-17β, progesterone, relaxin and chorionic gonadotrophin during blastocyst implantation in natural pregnancy cycle and in embryo transfer cycle in the rhesus monkey. / Ghosh, Debabrata; Stewart, Dennis R.; Nayak, Nihar Ranjan; Lasley, Bill L.; Overstreet, James W.; Hendrickx, Andrew G; Sengupta, Jayasree.

In: Human Reproduction, Vol. 12, No. 5, 1997, p. 914-920.

Research output: Contribution to journalArticle

Ghosh, Debabrata ; Stewart, Dennis R. ; Nayak, Nihar Ranjan ; Lasley, Bill L. ; Overstreet, James W. ; Hendrickx, Andrew G ; Sengupta, Jayasree. / Serum concentrations of oestradiol-17β, progesterone, relaxin and chorionic gonadotrophin during blastocyst implantation in natural pregnancy cycle and in embryo transfer cycle in the rhesus monkey. In: Human Reproduction. 1997 ; Vol. 12, No. 5. pp. 914-920.
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AU - Ghosh, Debabrata

AU - Stewart, Dennis R.

AU - Nayak, Nihar Ranjan

AU - Lasley, Bill L.

AU - Overstreet, James W.

AU - Hendrickx, Andrew G

AU - Sengupta, Jayasree

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AB - The present study was undertaken to assess the temporal association between the profiles of serum concentrations of oestradiol-17β, progesterone, chorionic gonadotrophin (CG) and relaxin in pregnancies established naturally, and after embryo transfer, as well as in failed pregnancies in rhesus monkeys. In naturally mated cycles (group 1) a conception rate of 75% was obtained. In group 1, the mean day of CG detection in serum was 11.5 ± 1.9 day post-ovulation, and for relaxin, 9.0 ± 2.5 day post-ovulation. In group 2, embryo transfer to synchronous, non-mated surrogate recipients was performed; seven embryo transfer cycles yielded three pregnancies which were allowed to continue to term and normal infants were delivered. In embryo transfer cycles the mean day of CG detection was 14.8 ± 1.8 day post-ovulation, and for relaxin, 11.4 ± 2.6 day post-ovulation. A delay of about 3 days was observed in the appearance in circulation of CG (P < 0.05) and also of relaxin (P < 0.05) between natural mated and embryo transfer conception cycles. Significant differences (P < 0.05 for progesterone and P < 0.03 for oestradiol) were obtained for the areas under the curves for progesterone and oestradiol between days 12 and 16 in conception cycles compared with failed pregnancies. These data provide the first observation of the normal hormonal signals associated with maternal recognition of transferred embryos during the peri-implantation period, and suggest that the use of such an experimental primate embryo transfer model may help to elucidate components of maternal and embryonic signal-response mechanisms during embryo implantation.

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