Serum biomarker levels for musculoskeletal disease in two- and three-year-old racing Thoroughbred horses: A prospective study of 130 horses

D. D. Frisbie, C. W. Mc Ilwraith, Rick Arthur, J. Blea, V. A. Baker, R. C. Billinghurst

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Reason for performing study: Biomarkers have shown some in vivo promise for the detection of musculoskeletal injuries, but further study to assess biomarker levels in clinical orthopaedic disease is required. Objective: To assess 7 serum biomarkers for the detection of musculoskeletal injuries. Methods: Two- and 3-year-old racehorses were entered into the study (n = 238). Exit criteria were lack of training for >30 days, or completion of 10 study months. Data from horses with solitary musculoskeletal injuries and completion of >2 months were analysed. Musculoskeletal injury was considered intra-articular fragmentation (IAF), tendon or ligamentous injury (TL), stress fractures (SF) and dorsal metacarpal disease (DMD). Monthly lameness examination and serum collection were performed. Serum was analysed for glycosaminoglycan (GAG), type I and II collagen degradation (C1, 2C), type II collagen synthesis (CPII), type II collagen degradation (Col CEQ), aggrecan synthesis (CS846), osteocalcin (OC) as a marker of bone formation and (C-terminal telopeptide of type I collagen) CTX as a marker of bone degradation. Results: Of the 238 horses 59 injured and 71 uninjured control horses met the analysis criteria. Based on injury no significant differences in the proportions were observed for age, gender or lesion type, although a higher proportion of injuries occurred at the beginning of the study. Of injured horses, 16 (27%) sustained an IAF, 17 (29%) a TL injury, 7 (12%) SF and 19 (32%) were diagnosed with DMD. There were significant changes seen in biomarkers based on the injury incurred when longitudinal samples were assessed. Furthermore, based on the serum biomarkers collected prior to injury, horses could be correctly classified as injured or uninjured 73.8% of the time. Conclusions: A unique biomarker pattern occurred before each injury and this was beneficial in classifying horses as injured or uninjured. Potential relevance: Biomarkers have the potential to be used as a screening aid prior to musculoskeletal injury.

Original languageEnglish (US)
Pages (from-to)643-651
Number of pages9
JournalEquine Veterinary Journal
Volume42
Issue number7
DOIs
StatePublished - Oct 1 2010

Fingerprint

Musculoskeletal Diseases
musculoskeletal diseases
prospective studies
Horses
biomarkers
Biomarkers
Prospective Studies
horses
Wounds and Injuries
Serum
collagen
stress fractures
metacarpus
Collagen Type II
degradation
Stress Fractures
Metacarpal Bones
osteocalcin
synthesis
racehorses

Keywords

  • Biomarkers
  • Clinical prospective
  • Horse
  • Musculoskeletal injuries

ASJC Scopus subject areas

  • Equine

Cite this

Serum biomarker levels for musculoskeletal disease in two- and three-year-old racing Thoroughbred horses : A prospective study of 130 horses. / Frisbie, D. D.; Mc Ilwraith, C. W.; Arthur, Rick; Blea, J.; Baker, V. A.; Billinghurst, R. C.

In: Equine Veterinary Journal, Vol. 42, No. 7, 01.10.2010, p. 643-651.

Research output: Contribution to journalArticle

Frisbie, D. D. ; Mc Ilwraith, C. W. ; Arthur, Rick ; Blea, J. ; Baker, V. A. ; Billinghurst, R. C. / Serum biomarker levels for musculoskeletal disease in two- and three-year-old racing Thoroughbred horses : A prospective study of 130 horses. In: Equine Veterinary Journal. 2010 ; Vol. 42, No. 7. pp. 643-651.
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