Serum and tissue pregnanes and pregnenes after dexamethasone treatment of cows in late gestation

Alan J Conley, Erin L. Legacki, C. Jo Corbin, Scott D Stanley, Carl R. Dahlen, Lawrence P. Reynolds

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dexamethasone (DEX) initiates parturition by inducing progesterone withdrawal and affecting placental steroidogenesis, but the effects of DEX in fetal and maternal tissue steroid synthetic capacity remains poorly investigated. Blood was collected from cows at 270 days of gestation before DEX or saline (SAL) treatment, and blood and tissues were collected at slaughter 38 h later. Steroid concentrations were determined by liquid chromatography tandem mass spectrometry to detect multiple steroids including 5α-reduced pregnane metabolites of progesterone. The activities of 3β-hydroxysteroid dehydrogenase (3βHSD) in cotyledonary and luteal microsomes and mitochondria and cotyledonary microsomal 5α-reductase were assessed. Quantitative PCR was used to further assess transcripts encoding enzymes and factors supporting steroidogenesis in cotyledonary and luteal tissues. Serum progesterone, pregnenolone, 5α-dihydroprogesterone (DHP) and allopregnanolone (3αDHP) concentrations (all <5 ng/mL before treatment) decreased in cows after DEX. However, the 20α-hydroxylated metabolite of DHP, 20αDHP, was higher before treatment (≈100 ng/ mL) than at slaughter but not affected by DEX. Serum, cotyledonary and luteal progesterone was lower in DEX- than SAL-treated cows. Progesterone was >100-fold higher in luteal than cotyledonary tissues, and serum and luteal concentrations were highly correlated in DEX-treated cows. 3βHSD activity was >5-fold higher in luteal than cotyledonary tissue, microsomes had more 3βHSD than mitochondria in luteal tissue but equal in cotyledonary sub-cellular fractions. DEX did not affect either luteal or cotyledonary 3βHSD activity but luteal steroidogenic enzyme transcripts were lower in DEX-treated cows. DEX induced functional luteal regression and progesterone withdrawal before any changes in placental pregnene/pregnane synthesis and/or metabolism were detectable.

Original languageEnglish (US)
Pages (from-to)413-422
Number of pages10
JournalReproduction
Volume157
Issue number5
DOIs
StatePublished - Jan 1 2019

Fingerprint

Pregnenes
Pregnanes
Corpus Luteum
Dexamethasone
3-Hydroxysteroid Dehydrogenases
Pregnancy
Serum
Progesterone
Steroids
Microsomes
Mitochondria
20-alpha-Dihydroprogesterone
Pregnanolone
Luteolysis
Pregnenolone
Enzymes
Tandem Mass Spectrometry
Liquid Chromatography
Oxidoreductases
Fetus

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Obstetrics and Gynecology
  • Cell Biology

Cite this

Serum and tissue pregnanes and pregnenes after dexamethasone treatment of cows in late gestation. / Conley, Alan J; Legacki, Erin L.; Jo Corbin, C.; Stanley, Scott D; Dahlen, Carl R.; Reynolds, Lawrence P.

In: Reproduction, Vol. 157, No. 5, 01.01.2019, p. 413-422.

Research output: Contribution to journalArticle

Conley, Alan J ; Legacki, Erin L. ; Jo Corbin, C. ; Stanley, Scott D ; Dahlen, Carl R. ; Reynolds, Lawrence P. / Serum and tissue pregnanes and pregnenes after dexamethasone treatment of cows in late gestation. In: Reproduction. 2019 ; Vol. 157, No. 5. pp. 413-422.
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