Abstract
The authors have used gel filtration chromatography to compare relative antibody affinities of liver ferritin and serum ferritin from patients with idiopathic hemochromatosis and other causes of hyperferritinemia such as liver disease, transfusional iron overload, and cancer. When equal amounts of either liver or serum ferritin were mixed with 125I-labeled antiferritin antibody then eluted through an agarose column, they obtained higher antigen-antibody complex recovery with the serum ferritin than liver ferritin. This was more marked in patients with untreated, idiopathic hemochromatosis. Their findings provide additional evidence of different reactivity between serum isoferritins and an antibody prepared against organ-specific ferritin. Further, this suggests that the immunoradiometric assay may underestimate serum ferritin concentration in patients with high ferritin values.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 444-448 |
| Number of pages | 5 |
| Journal | Proceedings of the Society for Experimental Biology and Medicine |
| Volume | 161 |
| Issue number | 4 |
| State | Published - 1979 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Serum and liver isoferritin differences : Evidence that serum ferritin may be underestimated in hyperferritinemia. / Saab, G. A.; Green, Ralph; Crosby, W. H.
In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 161, No. 4, 1979, p. 444-448.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Serum and liver isoferritin differences
T2 - Evidence that serum ferritin may be underestimated in hyperferritinemia
AU - Saab, G. A.
AU - Green, Ralph
AU - Crosby, W. H.
PY - 1979
Y1 - 1979
N2 - The authors have used gel filtration chromatography to compare relative antibody affinities of liver ferritin and serum ferritin from patients with idiopathic hemochromatosis and other causes of hyperferritinemia such as liver disease, transfusional iron overload, and cancer. When equal amounts of either liver or serum ferritin were mixed with 125I-labeled antiferritin antibody then eluted through an agarose column, they obtained higher antigen-antibody complex recovery with the serum ferritin than liver ferritin. This was more marked in patients with untreated, idiopathic hemochromatosis. Their findings provide additional evidence of different reactivity between serum isoferritins and an antibody prepared against organ-specific ferritin. Further, this suggests that the immunoradiometric assay may underestimate serum ferritin concentration in patients with high ferritin values.
AB - The authors have used gel filtration chromatography to compare relative antibody affinities of liver ferritin and serum ferritin from patients with idiopathic hemochromatosis and other causes of hyperferritinemia such as liver disease, transfusional iron overload, and cancer. When equal amounts of either liver or serum ferritin were mixed with 125I-labeled antiferritin antibody then eluted through an agarose column, they obtained higher antigen-antibody complex recovery with the serum ferritin than liver ferritin. This was more marked in patients with untreated, idiopathic hemochromatosis. Their findings provide additional evidence of different reactivity between serum isoferritins and an antibody prepared against organ-specific ferritin. Further, this suggests that the immunoradiometric assay may underestimate serum ferritin concentration in patients with high ferritin values.
UR - http://www.scopus.com/inward/record.url?scp=0018734599&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018734599&partnerID=8YFLogxK
M3 - Article
C2 - 482274
AN - SCOPUS:0018734599
VL - 161
SP - 444
EP - 448
JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
SN - 1535-3702
IS - 4
ER -