Serum and liver isoferritin differences: Evidence that serum ferritin may be underestimated in hyperferritinemia

G. A. Saab, Ralph Green, W. H. Crosby

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The authors have used gel filtration chromatography to compare relative antibody affinities of liver ferritin and serum ferritin from patients with idiopathic hemochromatosis and other causes of hyperferritinemia such as liver disease, transfusional iron overload, and cancer. When equal amounts of either liver or serum ferritin were mixed with 125I-labeled antiferritin antibody then eluted through an agarose column, they obtained higher antigen-antibody complex recovery with the serum ferritin than liver ferritin. This was more marked in patients with untreated, idiopathic hemochromatosis. Their findings provide additional evidence of different reactivity between serum isoferritins and an antibody prepared against organ-specific ferritin. Further, this suggests that the immunoradiometric assay may underestimate serum ferritin concentration in patients with high ferritin values.

Original languageEnglish (US)
Pages (from-to)444-448
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume161
Issue number4
StatePublished - 1979
Externally publishedYes

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Ferritins
Liver
Serum
Hemochromatosis
Antibodies
Immunoradiometric Assay
Antibody Affinity
Iron Overload
Chromatography
Antigen-Antibody Complex
Sepharose
Gel Chromatography
Liver Diseases
Assays
Iron
Gels
Recovery

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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abstract = "The authors have used gel filtration chromatography to compare relative antibody affinities of liver ferritin and serum ferritin from patients with idiopathic hemochromatosis and other causes of hyperferritinemia such as liver disease, transfusional iron overload, and cancer. When equal amounts of either liver or serum ferritin were mixed with 125I-labeled antiferritin antibody then eluted through an agarose column, they obtained higher antigen-antibody complex recovery with the serum ferritin than liver ferritin. This was more marked in patients with untreated, idiopathic hemochromatosis. Their findings provide additional evidence of different reactivity between serum isoferritins and an antibody prepared against organ-specific ferritin. Further, this suggests that the immunoradiometric assay may underestimate serum ferritin concentration in patients with high ferritin values.",
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T2 - Evidence that serum ferritin may be underestimated in hyperferritinemia

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AU - Green, Ralph

AU - Crosby, W. H.

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