Serum α-tocopherol, vitamin A, and blood selenium concentrations, and glutathione peroxidase activity in llamas fed alfalfa hay

Andrew J. Dart, Hailu Kinde, David R. Hodgson, John R. Peauroi, Arthur W. Selby, John Maas, Murray E. Fowler

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Abstract

Objectives - To measure blood selenium concentration and glutathione peroxidase (GSH-Px) activity and serum concentrations of vitamin A and α-tocopherol, and to determine the correlation between blood selenium concentration and GSH-Px activity of llamas fed alfalfa hay. Design - Mean (± SD) serum vitamin A and α-tocopherol concentrations, blood selenium concentrations, and GSH-Px activity were calculated from 9 sequential blood samples collected from llamas fed a diet of alfalfa hay. Animals - 15 clinically normal llamas (8 males, 7 females) between 10 and 14 months of age. Procedure - Llamas were fed alfalfa hay for 40 days prior to sample collection and then for the duration of the trial Vitamin E, selenium, and concentrations of vitamin A precursors were measured in the hay. Blood samples were collected on days 0, 6, 7, 9, 13, 20, 42, 64, and 98. Blood selenium concentrations were measured, using an inductively coupled spectrometric method. Blood GSH-Px activity was measured with a spectrophotometer, using a modification of a previously described assay, lsocratic high-performance liquid chromatography with florescent detection was used to determine serum α-tocopherol and vitamin A concentrations. Results - The alfalfa hay contained 0 2 mg/kg of selenium, 5 mg/kg of vitamin E, and 0.9 mg/kg of vitamin A precursors. The mean (± SD) blood selenium concentration and GSH-Px activity were 0.179 ± 0 032 μg/ml and 25 76 ± 6 53 mU NADPH oxidized/min/mg of Hb, respectively, with a correlation coefficient of 0.97. The mean (± SD) concentrations for serum α-tocopherol and vitamin A were 128.1 ± 41.7 and 74.8 ± 5.5 μg/dl, respectively. Conclusions - Blood selenium concentrations in llamas are highly correlated to GSH-Px activity. Blood selenium concentrations in llamas appear to be similar to other domestic ruminants and diets containing 0.2 mg/kg of selenium appear to provide an adequate dietary source. The concentrations of vitamin A precursors and vitamin E in the hay were below currently recommended dietary levels for llamas, and alfalfa hay appears to provide an unreliable source of vitamins A and E in this species. Further studies are required to determine optimal dietary concentrations and to substantiate a reference range for these vitamins in llamas.

Original languageEnglish (US)
Pages (from-to)689-692
Number of pages4
JournalAmerican Journal of Veterinary Research
Volume57
Issue number5
StatePublished - May 1996

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New World Camelids
llamas
Tocopherols
Medicago sativa
alfalfa hay
Selenium
Glutathione Peroxidase
blood serum
Vitamin A
tocopherols
glutathione peroxidase
selenium
vitamin A
blood
Serum
vitamin E
Vitamin E
hay
Diet
spectrophotometers

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Serum α-tocopherol, vitamin A, and blood selenium concentrations, and glutathione peroxidase activity in llamas fed alfalfa hay. / Dart, Andrew J.; Kinde, Hailu; Hodgson, David R.; Peauroi, John R.; Selby, Arthur W.; Maas, John; Fowler, Murray E.

In: American Journal of Veterinary Research, Vol. 57, No. 5, 05.1996, p. 689-692.

Research output: Contribution to journalArticle

Dart, Andrew J. ; Kinde, Hailu ; Hodgson, David R. ; Peauroi, John R. ; Selby, Arthur W. ; Maas, John ; Fowler, Murray E. / Serum α-tocopherol, vitamin A, and blood selenium concentrations, and glutathione peroxidase activity in llamas fed alfalfa hay. In: American Journal of Veterinary Research. 1996 ; Vol. 57, No. 5. pp. 689-692.
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abstract = "Objectives - To measure blood selenium concentration and glutathione peroxidase (GSH-Px) activity and serum concentrations of vitamin A and α-tocopherol, and to determine the correlation between blood selenium concentration and GSH-Px activity of llamas fed alfalfa hay. Design - Mean (± SD) serum vitamin A and α-tocopherol concentrations, blood selenium concentrations, and GSH-Px activity were calculated from 9 sequential blood samples collected from llamas fed a diet of alfalfa hay. Animals - 15 clinically normal llamas (8 males, 7 females) between 10 and 14 months of age. Procedure - Llamas were fed alfalfa hay for 40 days prior to sample collection and then for the duration of the trial Vitamin E, selenium, and concentrations of vitamin A precursors were measured in the hay. Blood samples were collected on days 0, 6, 7, 9, 13, 20, 42, 64, and 98. Blood selenium concentrations were measured, using an inductively coupled spectrometric method. Blood GSH-Px activity was measured with a spectrophotometer, using a modification of a previously described assay, lsocratic high-performance liquid chromatography with florescent detection was used to determine serum α-tocopherol and vitamin A concentrations. Results - The alfalfa hay contained 0 2 mg/kg of selenium, 5 mg/kg of vitamin E, and 0.9 mg/kg of vitamin A precursors. The mean (± SD) blood selenium concentration and GSH-Px activity were 0.179 ± 0 032 μg/ml and 25 76 ± 6 53 mU NADPH oxidized/min/mg of Hb, respectively, with a correlation coefficient of 0.97. The mean (± SD) concentrations for serum α-tocopherol and vitamin A were 128.1 ± 41.7 and 74.8 ± 5.5 μg/dl, respectively. Conclusions - Blood selenium concentrations in llamas are highly correlated to GSH-Px activity. Blood selenium concentrations in llamas appear to be similar to other domestic ruminants and diets containing 0.2 mg/kg of selenium appear to provide an adequate dietary source. The concentrations of vitamin A precursors and vitamin E in the hay were below currently recommended dietary levels for llamas, and alfalfa hay appears to provide an unreliable source of vitamins A and E in this species. Further studies are required to determine optimal dietary concentrations and to substantiate a reference range for these vitamins in llamas.",
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T1 - Serum α-tocopherol, vitamin A, and blood selenium concentrations, and glutathione peroxidase activity in llamas fed alfalfa hay

AU - Dart, Andrew J.

AU - Kinde, Hailu

AU - Hodgson, David R.

AU - Peauroi, John R.

AU - Selby, Arthur W.

AU - Maas, John

AU - Fowler, Murray E.

PY - 1996/5

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N2 - Objectives - To measure blood selenium concentration and glutathione peroxidase (GSH-Px) activity and serum concentrations of vitamin A and α-tocopherol, and to determine the correlation between blood selenium concentration and GSH-Px activity of llamas fed alfalfa hay. Design - Mean (± SD) serum vitamin A and α-tocopherol concentrations, blood selenium concentrations, and GSH-Px activity were calculated from 9 sequential blood samples collected from llamas fed a diet of alfalfa hay. Animals - 15 clinically normal llamas (8 males, 7 females) between 10 and 14 months of age. Procedure - Llamas were fed alfalfa hay for 40 days prior to sample collection and then for the duration of the trial Vitamin E, selenium, and concentrations of vitamin A precursors were measured in the hay. Blood samples were collected on days 0, 6, 7, 9, 13, 20, 42, 64, and 98. Blood selenium concentrations were measured, using an inductively coupled spectrometric method. Blood GSH-Px activity was measured with a spectrophotometer, using a modification of a previously described assay, lsocratic high-performance liquid chromatography with florescent detection was used to determine serum α-tocopherol and vitamin A concentrations. Results - The alfalfa hay contained 0 2 mg/kg of selenium, 5 mg/kg of vitamin E, and 0.9 mg/kg of vitamin A precursors. The mean (± SD) blood selenium concentration and GSH-Px activity were 0.179 ± 0 032 μg/ml and 25 76 ± 6 53 mU NADPH oxidized/min/mg of Hb, respectively, with a correlation coefficient of 0.97. The mean (± SD) concentrations for serum α-tocopherol and vitamin A were 128.1 ± 41.7 and 74.8 ± 5.5 μg/dl, respectively. Conclusions - Blood selenium concentrations in llamas are highly correlated to GSH-Px activity. Blood selenium concentrations in llamas appear to be similar to other domestic ruminants and diets containing 0.2 mg/kg of selenium appear to provide an adequate dietary source. The concentrations of vitamin A precursors and vitamin E in the hay were below currently recommended dietary levels for llamas, and alfalfa hay appears to provide an unreliable source of vitamins A and E in this species. Further studies are required to determine optimal dietary concentrations and to substantiate a reference range for these vitamins in llamas.

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