Seroprevalence of Psoroptes sp. mites in free-ranging elk (Cervus elaphus) as determined by kinetic ELISA

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Abstract

Western blots and a kinetic enzyme-linked immunosorbent assay (ELISA) were used to characterize and quantify the prevalence of antibodies to Psoroptes sp. mites in elk (Cervus elaphus) from nine herds in North America. Sera from infested (n = 18) and non-infested (n = 22) elk were used to optimize test methodology and to define cut-off values for negative, suspect, and positive samples. Among 357 samples, 35 (9.8%) of the animals were seropositive, 259 (73%) were negative, and 63 (18%) were suspect. Six of nine herds (67%) contained positive animals and two additional herds (22%) had suspect animals. Sex was not associated with prevalence of antibodies, but adults greater than 2 yr old were approximately five times more likely (95% confidence interval = 2.6-15.4) to be seropositive than calves. Based on these results, we propose that exposure to Psoroptes sp. mites may be widespread in free-ranging elk of North America.

Original languageEnglish (US)
Pages (from-to)51-56
Number of pages6
JournalJournal of Wildlife Diseases
Volume32
Issue number1
StatePublished - Jan 1996

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Psoroptidae
Psoroptes
elks
Mites
Seroepidemiologic Studies
Cervus elaphus
mite
seroprevalence
mites
Enzyme-Linked Immunosorbent Assay
herds
enzyme-linked immunosorbent assay
assay
enzyme
North America
kinetics
antibody
animal
animals
Antibodies

Keywords

  • Antibodies
  • Cervus elaphus
  • ELISA
  • Elk
  • Immunodiagnosis
  • Mites
  • Psoroptes sp.
  • Scabies
  • Serologic survey

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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title = "Seroprevalence of Psoroptes sp. mites in free-ranging elk (Cervus elaphus) as determined by kinetic ELISA",
abstract = "Western blots and a kinetic enzyme-linked immunosorbent assay (ELISA) were used to characterize and quantify the prevalence of antibodies to Psoroptes sp. mites in elk (Cervus elaphus) from nine herds in North America. Sera from infested (n = 18) and non-infested (n = 22) elk were used to optimize test methodology and to define cut-off values for negative, suspect, and positive samples. Among 357 samples, 35 (9.8{\%}) of the animals were seropositive, 259 (73{\%}) were negative, and 63 (18{\%}) were suspect. Six of nine herds (67{\%}) contained positive animals and two additional herds (22{\%}) had suspect animals. Sex was not associated with prevalence of antibodies, but adults greater than 2 yr old were approximately five times more likely (95{\%} confidence interval = 2.6-15.4) to be seropositive than calves. Based on these results, we propose that exposure to Psoroptes sp. mites may be widespread in free-ranging elk of North America.",
keywords = "Antibodies, Cervus elaphus, ELISA, Elk, Immunodiagnosis, Mites, Psoroptes sp., Scabies, Serologic survey",
author = "Ziccardi, {Michael H} and Boyce, {Walter M} and Ian Gardner",
year = "1996",
month = "1",
language = "English (US)",
volume = "32",
pages = "51--56",
journal = "Journal of Wildlife Diseases",
issn = "0090-3558",
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T1 - Seroprevalence of Psoroptes sp. mites in free-ranging elk (Cervus elaphus) as determined by kinetic ELISA

AU - Ziccardi, Michael H

AU - Boyce, Walter M

AU - Gardner, Ian

PY - 1996/1

Y1 - 1996/1

N2 - Western blots and a kinetic enzyme-linked immunosorbent assay (ELISA) were used to characterize and quantify the prevalence of antibodies to Psoroptes sp. mites in elk (Cervus elaphus) from nine herds in North America. Sera from infested (n = 18) and non-infested (n = 22) elk were used to optimize test methodology and to define cut-off values for negative, suspect, and positive samples. Among 357 samples, 35 (9.8%) of the animals were seropositive, 259 (73%) were negative, and 63 (18%) were suspect. Six of nine herds (67%) contained positive animals and two additional herds (22%) had suspect animals. Sex was not associated with prevalence of antibodies, but adults greater than 2 yr old were approximately five times more likely (95% confidence interval = 2.6-15.4) to be seropositive than calves. Based on these results, we propose that exposure to Psoroptes sp. mites may be widespread in free-ranging elk of North America.

AB - Western blots and a kinetic enzyme-linked immunosorbent assay (ELISA) were used to characterize and quantify the prevalence of antibodies to Psoroptes sp. mites in elk (Cervus elaphus) from nine herds in North America. Sera from infested (n = 18) and non-infested (n = 22) elk were used to optimize test methodology and to define cut-off values for negative, suspect, and positive samples. Among 357 samples, 35 (9.8%) of the animals were seropositive, 259 (73%) were negative, and 63 (18%) were suspect. Six of nine herds (67%) contained positive animals and two additional herds (22%) had suspect animals. Sex was not associated with prevalence of antibodies, but adults greater than 2 yr old were approximately five times more likely (95% confidence interval = 2.6-15.4) to be seropositive than calves. Based on these results, we propose that exposure to Psoroptes sp. mites may be widespread in free-ranging elk of North America.

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KW - ELISA

KW - Elk

KW - Immunodiagnosis

KW - Mites

KW - Psoroptes sp.

KW - Scabies

KW - Serologic survey

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