TY - JOUR
T1 - Serological Evidence for Henipa-like and Filo-like Viruses in Trinidad Bats
AU - Schulz, Jonathan E.
AU - Seifert, Stephanie N.
AU - Thompson, John T.
AU - Avanzato, Victoria
AU - Sterling, Spencer L.
AU - Yan, Lianying
AU - Letko, Michael C.
AU - Matson, M. Jeremiah
AU - Fischer, Robert J.
AU - Tremeau-Bravard, Alexandre
AU - Seetahal, Janine F.R.
AU - Ramkissoon, Vernie
AU - Foster, Jerome
AU - Goldstein, Tracey
AU - Anthony, Simon J.
AU - Epstein, Jonathan H.
AU - Laing, Eric D.
AU - Broder, Christopher C.
AU - Carrington, Christine V.F.
AU - Schountz, Tony
AU - Munster, Vincent J.
PY - 2020/5/11
Y1 - 2020/5/11
N2 - Bat-borne zoonotic pathogens belonging to the family Paramxyoviridae, including Nipah and Hendra viruses, and the family Filoviridae, including Ebola and Marburg viruses, can cause severe disease and high mortality rates on spillover into human populations. Surveillance efforts for henipaviruses and filoviruses have been largely restricted to the Old World; however, recent studies suggest a potentially broader distribution for henipaviruses and filoviruses than previously recognized. In the current study, we screened for henipaviruses and filoviruses in New World bats collected across 4 locations in Trinidad near the coast of Venezuela. Bat tissue samples were screened using previously established reverse-transcription polymerase chain reaction assays. Serum were screened using a multiplex immunoassay to detect antibodies reactive with the envelope glycoprotein of viruses in the genus Henipavirus and the family Filoviridae. Serum samples were also screened by means of enzyme-linked immunosorbent assay for antibodies reactive with Nipah G and F glycoproteins. Of 84 serum samples, 28 were reactive with ≥1 henipavirus glycoprotein by ≥1 serological method, and 6 serum samples were reactive against ≥1 filovirus glycoproteins. These data provide evidence of potential circulation of viruses related to the henipaviruses and filoviruses in New World bats.
AB - Bat-borne zoonotic pathogens belonging to the family Paramxyoviridae, including Nipah and Hendra viruses, and the family Filoviridae, including Ebola and Marburg viruses, can cause severe disease and high mortality rates on spillover into human populations. Surveillance efforts for henipaviruses and filoviruses have been largely restricted to the Old World; however, recent studies suggest a potentially broader distribution for henipaviruses and filoviruses than previously recognized. In the current study, we screened for henipaviruses and filoviruses in New World bats collected across 4 locations in Trinidad near the coast of Venezuela. Bat tissue samples were screened using previously established reverse-transcription polymerase chain reaction assays. Serum were screened using a multiplex immunoassay to detect antibodies reactive with the envelope glycoprotein of viruses in the genus Henipavirus and the family Filoviridae. Serum samples were also screened by means of enzyme-linked immunosorbent assay for antibodies reactive with Nipah G and F glycoproteins. Of 84 serum samples, 28 were reactive with ≥1 henipavirus glycoprotein by ≥1 serological method, and 6 serum samples were reactive against ≥1 filovirus glycoproteins. These data provide evidence of potential circulation of viruses related to the henipaviruses and filoviruses in New World bats.
KW - Bats
KW - Filovirus
KW - Henipavirus
KW - Luminex
KW - RT-PCR
KW - Screening
KW - Serology
KW - Trinidad
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U2 - 10.1093/infdis/jiz648
DO - 10.1093/infdis/jiz648
M3 - Article
C2 - 32034942
AN - SCOPUS:85084695168
VL - 221
SP - S375-S382
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 4
ER -