Serological confirmation of human T-lymphotropic virus type I infection in healthy blood and plasma donors

D. W. Anderson, J. S. Epstein, T. H. Lee, Michael Dale Lairmore, C. Saxinger, V. S. Kalyanaraman, D. Slamon, W. Parks, B. J. Poiesz, L. T. Pierik, H. Lee, R. Montagna, P. A. Roche, A. Williams, W. Blattner

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Abstract

We wished to develop criteria for serological confirmation of human T-lymphotropic virus type I (HTLV-I) infection in healthy donors. Selected serum or plasma samples reactive by HTLV-I enzyme immunosorbent assay or gel-agglutination assays with at least one viral-specific band on Western immunoblot (WIB) were tested in six laboratories by four WIBs and four radioimmunoprecipitation assays (RIPAs) for antibodies to HTLV-I proteins encoded by gag (p19 and p24), env (gp46 and/or gp61), and tax (p40(x)) genes. One hundred forty-two donor sera were obtained from 38 Japanese, 69 American, and 35 Caribbean blood or plasma donors. Among these samples, WIB assays appeared more sensitive to p24 antibodies, whereas RIPAs were significantly more sensitive to gp61 antibodies. All sera (137) with gp61 antibodies had p24 antibodies. Of the 137 sera positive for p24 and gp61 antibodies, p19 antibodies were detected in V129 sera, and p40(x) antibodies were detected in 108. In sera with p19 antibodies and antobodies to env- or tax-encoded proteins, p24 antibodies were always present. Antibodies to p40(x) were not found in the absence of gp61 antibodies. Virological evidence of infection was found in seven American donors by lymphocyte coculture (one HTLV-I, one HTLV-II) or by polymerase chain reaction (three HTLV-I, two HTLV-II). Sera from all seven donors showed p24 and gp46 and/or gp61 antibodies. We suggest that seroreactivity to both p24 and gp46 and/or gp61 by WIB or IPA or both are suitable criteria to confirm but not to distinguish HTLV-I and HTLV-II infections.

Original languageEnglish (US)
Pages (from-to)2585-2591
Number of pages7
JournalBlood
Volume74
Issue number7
StatePublished - 1989
Externally publishedYes

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Virus Diseases
Blood Donors
Viruses
Blood
Plasmas
Antibodies
Assays
Tissue Donors
Serum
Radioimmunoprecipitation Assay
tax Gene Products
Human T-lymphotropic virus 2
Western Blotting
HTLV-II Infections
gag Gene Products
Immunosorbents
Lymphocytes
Polymerase chain reaction
Agglutination
Enzyme Assays

ASJC Scopus subject areas

  • Hematology

Cite this

Anderson, D. W., Epstein, J. S., Lee, T. H., Lairmore, M. D., Saxinger, C., Kalyanaraman, V. S., ... Blattner, W. (1989). Serological confirmation of human T-lymphotropic virus type I infection in healthy blood and plasma donors. Blood, 74(7), 2585-2591.

Serological confirmation of human T-lymphotropic virus type I infection in healthy blood and plasma donors. / Anderson, D. W.; Epstein, J. S.; Lee, T. H.; Lairmore, Michael Dale; Saxinger, C.; Kalyanaraman, V. S.; Slamon, D.; Parks, W.; Poiesz, B. J.; Pierik, L. T.; Lee, H.; Montagna, R.; Roche, P. A.; Williams, A.; Blattner, W.

In: Blood, Vol. 74, No. 7, 1989, p. 2585-2591.

Research output: Contribution to journalArticle

Anderson, DW, Epstein, JS, Lee, TH, Lairmore, MD, Saxinger, C, Kalyanaraman, VS, Slamon, D, Parks, W, Poiesz, BJ, Pierik, LT, Lee, H, Montagna, R, Roche, PA, Williams, A & Blattner, W 1989, 'Serological confirmation of human T-lymphotropic virus type I infection in healthy blood and plasma donors', Blood, vol. 74, no. 7, pp. 2585-2591.
Anderson DW, Epstein JS, Lee TH, Lairmore MD, Saxinger C, Kalyanaraman VS et al. Serological confirmation of human T-lymphotropic virus type I infection in healthy blood and plasma donors. Blood. 1989;74(7):2585-2591.
Anderson, D. W. ; Epstein, J. S. ; Lee, T. H. ; Lairmore, Michael Dale ; Saxinger, C. ; Kalyanaraman, V. S. ; Slamon, D. ; Parks, W. ; Poiesz, B. J. ; Pierik, L. T. ; Lee, H. ; Montagna, R. ; Roche, P. A. ; Williams, A. ; Blattner, W. / Serological confirmation of human T-lymphotropic virus type I infection in healthy blood and plasma donors. In: Blood. 1989 ; Vol. 74, No. 7. pp. 2585-2591.
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abstract = "We wished to develop criteria for serological confirmation of human T-lymphotropic virus type I (HTLV-I) infection in healthy donors. Selected serum or plasma samples reactive by HTLV-I enzyme immunosorbent assay or gel-agglutination assays with at least one viral-specific band on Western immunoblot (WIB) were tested in six laboratories by four WIBs and four radioimmunoprecipitation assays (RIPAs) for antibodies to HTLV-I proteins encoded by gag (p19 and p24), env (gp46 and/or gp61), and tax (p40(x)) genes. One hundred forty-two donor sera were obtained from 38 Japanese, 69 American, and 35 Caribbean blood or plasma donors. Among these samples, WIB assays appeared more sensitive to p24 antibodies, whereas RIPAs were significantly more sensitive to gp61 antibodies. All sera (137) with gp61 antibodies had p24 antibodies. Of the 137 sera positive for p24 and gp61 antibodies, p19 antibodies were detected in V129 sera, and p40(x) antibodies were detected in 108. In sera with p19 antibodies and antobodies to env- or tax-encoded proteins, p24 antibodies were always present. Antibodies to p40(x) were not found in the absence of gp61 antibodies. Virological evidence of infection was found in seven American donors by lymphocyte coculture (one HTLV-I, one HTLV-II) or by polymerase chain reaction (three HTLV-I, two HTLV-II). Sera from all seven donors showed p24 and gp46 and/or gp61 antibodies. We suggest that seroreactivity to both p24 and gp46 and/or gp61 by WIB or IPA or both are suitable criteria to confirm but not to distinguish HTLV-I and HTLV-II infections.",
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AU - Anderson, D. W.

AU - Epstein, J. S.

AU - Lee, T. H.

AU - Lairmore, Michael Dale

AU - Saxinger, C.

AU - Kalyanaraman, V. S.

AU - Slamon, D.

AU - Parks, W.

AU - Poiesz, B. J.

AU - Pierik, L. T.

AU - Lee, H.

AU - Montagna, R.

AU - Roche, P. A.

AU - Williams, A.

AU - Blattner, W.

PY - 1989

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N2 - We wished to develop criteria for serological confirmation of human T-lymphotropic virus type I (HTLV-I) infection in healthy donors. Selected serum or plasma samples reactive by HTLV-I enzyme immunosorbent assay or gel-agglutination assays with at least one viral-specific band on Western immunoblot (WIB) were tested in six laboratories by four WIBs and four radioimmunoprecipitation assays (RIPAs) for antibodies to HTLV-I proteins encoded by gag (p19 and p24), env (gp46 and/or gp61), and tax (p40(x)) genes. One hundred forty-two donor sera were obtained from 38 Japanese, 69 American, and 35 Caribbean blood or plasma donors. Among these samples, WIB assays appeared more sensitive to p24 antibodies, whereas RIPAs were significantly more sensitive to gp61 antibodies. All sera (137) with gp61 antibodies had p24 antibodies. Of the 137 sera positive for p24 and gp61 antibodies, p19 antibodies were detected in V129 sera, and p40(x) antibodies were detected in 108. In sera with p19 antibodies and antobodies to env- or tax-encoded proteins, p24 antibodies were always present. Antibodies to p40(x) were not found in the absence of gp61 antibodies. Virological evidence of infection was found in seven American donors by lymphocyte coculture (one HTLV-I, one HTLV-II) or by polymerase chain reaction (three HTLV-I, two HTLV-II). Sera from all seven donors showed p24 and gp46 and/or gp61 antibodies. We suggest that seroreactivity to both p24 and gp46 and/or gp61 by WIB or IPA or both are suitable criteria to confirm but not to distinguish HTLV-I and HTLV-II infections.

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