Serological Assessment for Leishmania donovani Infection in Blood Donors of Sunsari District, Dharan, Nepal

Suraj Timilsina, Narayan Raj Bhattarai, Basudha Khanal, Suman Rijal

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Visceral leishmaniasis (VL) is a major vector-borne disease caused by Leishmania donovani, after replication of the parasites in macrophages, mononuclear phagocytic system. VL is endemic in 12 districts of central and eastern Terai lowlands of Nepal bordering North Bihar, India with an estimated 8 million population at risk. In addition, VL endemicity is also extending to new endemic regions like Dharan from its classical rural foci. Hence, we aimed to detect the evidence of Leishmania donovani infection in the blood samples received from blood donors of Sunsari district, Dharan, (eastern Nepal), a region endemic for human VL. Sera from 507 asymptomatic blood donors were subjected to serological screening for anti-Leishmania donovani antibodies. Direct agglutination test (DAT) was performed on the sera. Out of 507 donors, majority (78.50 %) were male. Among the donors, 472 (93.10 %) belonged to age group 18–45 years where as 35 (6.90 %) to age group >45 years. Circulating anti-Leishmania antibodies were detected in 5 (1 %) out of 507 healthy, Human Immunodeficiency Virus types 1 and 2 (HIV 1and 2), Hepatitis B Surface Antigen (HBsAg), anti- Hepatitis C Virus (anti-HCV)-negative, and Syphillis non-reactive donors. All the seropositive cases were male and belonged to the age group 18–45 years. The result suggests that there is an immediate need of screening asymptomatic blood donors for leishmania seropositivity especially in endemic areas.

Original languageEnglish (US)
Pages (from-to)95-99
Number of pages5
JournalIndian Journal of Hematology and Blood Transfusion
Volume32
Issue number1
DOIs
StatePublished - Mar 1 2016
Externally publishedYes

Fingerprint

Leishmania donovani
Nepal
Visceral Leishmaniasis
Blood Donors
Age Groups
Leishmania
Tissue Donors
Infection
Disease Vectors
Agglutination Tests
HIV-2
Hepatitis B Surface Antigens
Serum
Hepacivirus
HIV-1
India
Anti-Idiotypic Antibodies
Parasites
Macrophages
Antibodies

Keywords

  • Asymptomatic blood donors
  • DAT
  • Dharan
  • Visceral leishmaniasis

ASJC Scopus subject areas

  • Hematology

Cite this

Serological Assessment for Leishmania donovani Infection in Blood Donors of Sunsari District, Dharan, Nepal. / Timilsina, Suraj; Raj Bhattarai, Narayan; Khanal, Basudha; Rijal, Suman.

In: Indian Journal of Hematology and Blood Transfusion, Vol. 32, No. 1, 01.03.2016, p. 95-99.

Research output: Contribution to journalArticle

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