Serologic detection of subtype-specific antibodies to influenza A viruses in southern sea otters (Enhydra lutris nereis)

Alyssa M. Capuano, Melissa Miller, David E. Stallknecht, Megan Moriarty, Magdalena Plancarte, Erin Dodd, Francesca Batac, Walter M Boyce

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

There are approximately 3,000 southern sea otters (Enhydra lutris nereis) in the nearshore environment along the California coast, US, and the species is classified as Threatened under the Endangered Species Act. We tested sera from 661 necropsied southern sea otters sampled from 1997 to 2015 to determine overall exposure to influenza A viruses (IAVs) and to identify subtype-specific antibody responses. Using an enzyme-linked immunosorbent assay (ELISA), antibodies to IAV nucleoproteins were detected in 160 (24.2%) otters, with seropositive animals found in every year except 2008. When the ELISA-positive samples were tested by virus microneutralization, antibody responses were detected to avian-origin hemagglutinin subtypes H1, H3, H4, H5, H6, H7, H9, and H11. Strong anti body responses to pandemic H1N1 (pdmH1N1) were also detected, indicating that epizootic transmission of pdmH1N1 occurred among the southern sea otter population after the emergence of this human-origin virus in 2009. We conclude that southern sea otters are susceptible to infection with avian and human-origin IAV and that exposure to a wide array of subtypes likely occurs during a given otter’s 10- to 15-yr life span. Important unanswered questions include what effect, if any, IAV infection has on sea otter health, and how these animals become infected in their nearshore environment.

Original languageEnglish (US)
Pages (from-to)906-910
Number of pages5
JournalJournal of Wildlife Diseases
Volume53
Issue number4
DOIs
StatePublished - Oct 1 2017

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influenza
Influenza A virus
antibody
virus
antibodies
pandemic
nearshore environment
enzyme-linked immunosorbent assay
Enhydra lutris
viruses
nucleoproteins
assay
enzyme
biological resistance
hemagglutinins
animal
endangered species
animals
sea
detection

Keywords

  • Enhydra lutris nereis
  • Influenza A virus
  • Serology
  • Southern sea otter
  • Virus microneutralization

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Serologic detection of subtype-specific antibodies to influenza A viruses in southern sea otters (Enhydra lutris nereis). / Capuano, Alyssa M.; Miller, Melissa; Stallknecht, David E.; Moriarty, Megan; Plancarte, Magdalena; Dodd, Erin; Batac, Francesca; Boyce, Walter M.

In: Journal of Wildlife Diseases, Vol. 53, No. 4, 01.10.2017, p. 906-910.

Research output: Contribution to journalArticle

Capuano, Alyssa M. ; Miller, Melissa ; Stallknecht, David E. ; Moriarty, Megan ; Plancarte, Magdalena ; Dodd, Erin ; Batac, Francesca ; Boyce, Walter M. / Serologic detection of subtype-specific antibodies to influenza A viruses in southern sea otters (Enhydra lutris nereis). In: Journal of Wildlife Diseases. 2017 ; Vol. 53, No. 4. pp. 906-910.
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