Serologic cross-reactivity between Anaplasma marginale and Anaplasma phagocytophilum

U. M. Dreher, J. De La Fuente, R. Hofmann-Lehmann, M. L. Meli, Nicola Pusterla, K. M. Kocan, Z. Woldehiwet, U. Braun, G. Regula, K. D C Staerk, H. Lutz

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Abstract

In the context of a serosurvey conducted on the Anaplasma marginale prevalence in Swiss cattle, we suspected that a serological cross-reactivity between A. marginale and A. phagocytophilum might exist. In the present study we demonstrate that cattle, sheep and horses experimentally infected with A. phagocytophilum not only develop antibodies to A. phagocytophilum (detected by immunofluorescent-antibody assay) but also to A. marginale (detected by a competitive enzyme-linked immunosorbent assay). Conversely, calves experimentally infected with A. marginale also developed antibodies to A. phagocytophilum using the same serological tests. The identity of 63% determined in silico within a 209-amino-acid sequence of major surface protein 5 of an isolate of A. marginale and one of A. phagocytophilum supported the observed immunological cross-reactivity. These observations have important consequences for the serotesting of both, A. marginale and A. phagocytophilum infection of several animal species. In view of these new findings, tests that have been considered specific for either infection must be interpreted carefully.

Original languageEnglish (US)
Pages (from-to)1177-1183
Number of pages7
JournalClinical and Diagnostic Laboratory Immunology
Volume12
Issue number10
DOIs
StatePublished - Oct 2005

Fingerprint

Anaplasma marginale
Anaplasma phagocytophilum
Antibodies
Assays
Immunosorbents
Membrane Proteins
Animals
Amino Acids
Serologic Tests
Enzymes
Infection
Computer Simulation
Horses
Amino Acid Sequence
Sheep
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

Serologic cross-reactivity between Anaplasma marginale and Anaplasma phagocytophilum. / Dreher, U. M.; De La Fuente, J.; Hofmann-Lehmann, R.; Meli, M. L.; Pusterla, Nicola; Kocan, K. M.; Woldehiwet, Z.; Braun, U.; Regula, G.; Staerk, K. D C; Lutz, H.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 12, No. 10, 10.2005, p. 1177-1183.

Research output: Contribution to journalArticle

Dreher, UM, De La Fuente, J, Hofmann-Lehmann, R, Meli, ML, Pusterla, N, Kocan, KM, Woldehiwet, Z, Braun, U, Regula, G, Staerk, KDC & Lutz, H 2005, 'Serologic cross-reactivity between Anaplasma marginale and Anaplasma phagocytophilum', Clinical and Diagnostic Laboratory Immunology, vol. 12, no. 10, pp. 1177-1183. https://doi.org/10.1128/CDLI.12.10.1177-1183.2005
Dreher, U. M. ; De La Fuente, J. ; Hofmann-Lehmann, R. ; Meli, M. L. ; Pusterla, Nicola ; Kocan, K. M. ; Woldehiwet, Z. ; Braun, U. ; Regula, G. ; Staerk, K. D C ; Lutz, H. / Serologic cross-reactivity between Anaplasma marginale and Anaplasma phagocytophilum. In: Clinical and Diagnostic Laboratory Immunology. 2005 ; Vol. 12, No. 10. pp. 1177-1183.
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