Serologic confirmation of simian T-lymphotropic virus type I infection by using immunoassays developed for human T-lymphotropic virus antibody detection

D. L. Rudolph, J. Yee, J. Mone, S. K H Foung, J. J. Lipka, G. R. Reyes, K. Hadlock, L. Chan, F. Villinger, Michael Dale Lairmore, S. Sinha, R. B. Lal

Research output: Contribution to journalArticle

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Abstract

Serum specimens from diverse species of Old World monkeys, categorized as seropositive (n = 97) or seronegative (n = 23) for human T-lymphotropic virus (HTLV) infection, were tested by using recombinant env-spiked Western immunoblot assays and synthetic peptide assays for simultaneous detection and discrimination of simian T-lymphotropic virus (STLV) infection. Of the 97 seropositive specimens, 93 reacted with the recombinant transmembrane (r21(env)) protein and 90 reacted with a recombinant, MTA-1, derived from the central region of the external glycoprotein of HTLV-1 (rgp46(env)), thus yielding test sensitivities of 96 and 93%, respectively. While 1 of the 23 negative monkey specimens reacted with r21(env), none reacted with rgp46(env), for overall specificities of 96 and 100%, respectively. Analysis of synthetic peptide-based immunoassays demonstrated that while 85 of 97 (88%) seropositive specimens reacted with HTLV-I-specific epitope (p19(gag)), none of the specimens reacted with HTLV-II-specific epitope (gp52(env)). These results show that recombinant envelope-spiked Western blots provide a simple means for serologic confirmation of STLV-I infection and that type- specific synthetic peptides can be used to confirm the virus type in seropositive monkey specimens.

Original languageEnglish (US)
Pages (from-to)858-861
Number of pages4
JournalJournal of Clinical Microbiology
Volume30
Issue number4
StatePublished - 1992
Externally publishedYes

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Simian T-lymphotropic virus 1
Virus Diseases
Immunoassay
Viruses
Peptides
Haplorhini
Pemetrexed
Antibodies
Epitopes
Western Blotting
Human T-lymphotropic virus 2
Cercopithecidae
env Gene Products
Human T-lymphotropic virus 1
Glycoproteins
Serum

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Rudolph, D. L., Yee, J., Mone, J., Foung, S. K. H., Lipka, J. J., Reyes, G. R., ... Lal, R. B. (1992). Serologic confirmation of simian T-lymphotropic virus type I infection by using immunoassays developed for human T-lymphotropic virus antibody detection. Journal of Clinical Microbiology, 30(4), 858-861.

Serologic confirmation of simian T-lymphotropic virus type I infection by using immunoassays developed for human T-lymphotropic virus antibody detection. / Rudolph, D. L.; Yee, J.; Mone, J.; Foung, S. K H; Lipka, J. J.; Reyes, G. R.; Hadlock, K.; Chan, L.; Villinger, F.; Lairmore, Michael Dale; Sinha, S.; Lal, R. B.

In: Journal of Clinical Microbiology, Vol. 30, No. 4, 1992, p. 858-861.

Research output: Contribution to journalArticle

Rudolph, DL, Yee, J, Mone, J, Foung, SKH, Lipka, JJ, Reyes, GR, Hadlock, K, Chan, L, Villinger, F, Lairmore, MD, Sinha, S & Lal, RB 1992, 'Serologic confirmation of simian T-lymphotropic virus type I infection by using immunoassays developed for human T-lymphotropic virus antibody detection', Journal of Clinical Microbiology, vol. 30, no. 4, pp. 858-861.
Rudolph, D. L. ; Yee, J. ; Mone, J. ; Foung, S. K H ; Lipka, J. J. ; Reyes, G. R. ; Hadlock, K. ; Chan, L. ; Villinger, F. ; Lairmore, Michael Dale ; Sinha, S. ; Lal, R. B. / Serologic confirmation of simian T-lymphotropic virus type I infection by using immunoassays developed for human T-lymphotropic virus antibody detection. In: Journal of Clinical Microbiology. 1992 ; Vol. 30, No. 4. pp. 858-861.
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abstract = "Serum specimens from diverse species of Old World monkeys, categorized as seropositive (n = 97) or seronegative (n = 23) for human T-lymphotropic virus (HTLV) infection, were tested by using recombinant env-spiked Western immunoblot assays and synthetic peptide assays for simultaneous detection and discrimination of simian T-lymphotropic virus (STLV) infection. Of the 97 seropositive specimens, 93 reacted with the recombinant transmembrane (r21(env)) protein and 90 reacted with a recombinant, MTA-1, derived from the central region of the external glycoprotein of HTLV-1 (rgp46(env)), thus yielding test sensitivities of 96 and 93{\%}, respectively. While 1 of the 23 negative monkey specimens reacted with r21(env), none reacted with rgp46(env), for overall specificities of 96 and 100{\%}, respectively. Analysis of synthetic peptide-based immunoassays demonstrated that while 85 of 97 (88{\%}) seropositive specimens reacted with HTLV-I-specific epitope (p19(gag)), none of the specimens reacted with HTLV-II-specific epitope (gp52(env)). These results show that recombinant envelope-spiked Western blots provide a simple means for serologic confirmation of STLV-I infection and that type- specific synthetic peptides can be used to confirm the virus type in seropositive monkey specimens.",
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AU - Foung, S. K H

AU - Lipka, J. J.

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