Abstract
Serum specimens from diverse species of Old World monkeys, categorized as seropositive (n = 97) or seronegative (n = 23) for human T-lymphotropic virus (HTLV) infection, were tested by using recombinant env-spiked Western immunoblot assays and synthetic peptide assays for simultaneous detection and discrimination of simian T-lymphotropic virus (STLV) infection. Of the 97 seropositive specimens, 93 reacted with the recombinant transmembrane (r21(env)) protein and 90 reacted with a recombinant, MTA-1, derived from the central region of the external glycoprotein of HTLV-1 (rgp46(env)), thus yielding test sensitivities of 96 and 93%, respectively. While 1 of the 23 negative monkey specimens reacted with r21(env), none reacted with rgp46(env), for overall specificities of 96 and 100%, respectively. Analysis of synthetic peptide-based immunoassays demonstrated that while 85 of 97 (88%) seropositive specimens reacted with HTLV-I-specific epitope (p19(gag)), none of the specimens reacted with HTLV-II-specific epitope (gp52(env)). These results show that recombinant envelope-spiked Western blots provide a simple means for serologic confirmation of STLV-I infection and that type- specific synthetic peptides can be used to confirm the virus type in seropositive monkey specimens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 858-861 |
| Number of pages | 4 |
| Journal | Journal of Clinical Microbiology |
| Volume | 30 |
| Issue number | 4 |
| State | Published - 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)
Cite this
Serologic confirmation of simian T-lymphotropic virus type I infection by using immunoassays developed for human T-lymphotropic virus antibody detection. / Rudolph, D. L.; Yee, J.; Mone, J.; Foung, S. K H; Lipka, J. J.; Reyes, G. R.; Hadlock, K.; Chan, L.; Villinger, F.; Lairmore, Michael Dale; Sinha, S.; Lal, R. B.
In: Journal of Clinical Microbiology, Vol. 30, No. 4, 1992, p. 858-861.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Serologic confirmation of simian T-lymphotropic virus type I infection by using immunoassays developed for human T-lymphotropic virus antibody detection
AU - Rudolph, D. L.
AU - Yee, J.
AU - Mone, J.
AU - Foung, S. K H
AU - Lipka, J. J.
AU - Reyes, G. R.
AU - Hadlock, K.
AU - Chan, L.
AU - Villinger, F.
AU - Lairmore, Michael Dale
AU - Sinha, S.
AU - Lal, R. B.
PY - 1992
Y1 - 1992
N2 - Serum specimens from diverse species of Old World monkeys, categorized as seropositive (n = 97) or seronegative (n = 23) for human T-lymphotropic virus (HTLV) infection, were tested by using recombinant env-spiked Western immunoblot assays and synthetic peptide assays for simultaneous detection and discrimination of simian T-lymphotropic virus (STLV) infection. Of the 97 seropositive specimens, 93 reacted with the recombinant transmembrane (r21(env)) protein and 90 reacted with a recombinant, MTA-1, derived from the central region of the external glycoprotein of HTLV-1 (rgp46(env)), thus yielding test sensitivities of 96 and 93%, respectively. While 1 of the 23 negative monkey specimens reacted with r21(env), none reacted with rgp46(env), for overall specificities of 96 and 100%, respectively. Analysis of synthetic peptide-based immunoassays demonstrated that while 85 of 97 (88%) seropositive specimens reacted with HTLV-I-specific epitope (p19(gag)), none of the specimens reacted with HTLV-II-specific epitope (gp52(env)). These results show that recombinant envelope-spiked Western blots provide a simple means for serologic confirmation of STLV-I infection and that type- specific synthetic peptides can be used to confirm the virus type in seropositive monkey specimens.
AB - Serum specimens from diverse species of Old World monkeys, categorized as seropositive (n = 97) or seronegative (n = 23) for human T-lymphotropic virus (HTLV) infection, were tested by using recombinant env-spiked Western immunoblot assays and synthetic peptide assays for simultaneous detection and discrimination of simian T-lymphotropic virus (STLV) infection. Of the 97 seropositive specimens, 93 reacted with the recombinant transmembrane (r21(env)) protein and 90 reacted with a recombinant, MTA-1, derived from the central region of the external glycoprotein of HTLV-1 (rgp46(env)), thus yielding test sensitivities of 96 and 93%, respectively. While 1 of the 23 negative monkey specimens reacted with r21(env), none reacted with rgp46(env), for overall specificities of 96 and 100%, respectively. Analysis of synthetic peptide-based immunoassays demonstrated that while 85 of 97 (88%) seropositive specimens reacted with HTLV-I-specific epitope (p19(gag)), none of the specimens reacted with HTLV-II-specific epitope (gp52(env)). These results show that recombinant envelope-spiked Western blots provide a simple means for serologic confirmation of STLV-I infection and that type- specific synthetic peptides can be used to confirm the virus type in seropositive monkey specimens.
UR - http://www.scopus.com/inward/record.url?scp=0026513629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026513629&partnerID=8YFLogxK
M3 - Article
C2 - 1349306
AN - SCOPUS:0026513629
VL - 30
SP - 858
EP - 861
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 4
ER -