Serologic confirmation of simian T-lymphotropic virus type I infection by using immunoassays developed for human T-lymphotropic virus antibody detection

D. L. Rudolph, J. Yee, J. Mone, S. K H Foung, J. J. Lipka, G. R. Reyes, K. Hadlock, L. Chan, F. Villinger, Michael Dale Lairmore, S. Sinha, R. B. Lal

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Serum specimens from diverse species of Old World monkeys, categorized as seropositive (n = 97) or seronegative (n = 23) for human T-lymphotropic virus (HTLV) infection, were tested by using recombinant env-spiked Western immunoblot assays and synthetic peptide assays for simultaneous detection and discrimination of simian T-lymphotropic virus (STLV) infection. Of the 97 seropositive specimens, 93 reacted with the recombinant transmembrane (r21(env)) protein and 90 reacted with a recombinant, MTA-1, derived from the central region of the external glycoprotein of HTLV-1 (rgp46(env)), thus yielding test sensitivities of 96 and 93%, respectively. While 1 of the 23 negative monkey specimens reacted with r21(env), none reacted with rgp46(env), for overall specificities of 96 and 100%, respectively. Analysis of synthetic peptide-based immunoassays demonstrated that while 85 of 97 (88%) seropositive specimens reacted with HTLV-I-specific epitope (p19(gag)), none of the specimens reacted with HTLV-II-specific epitope (gp52(env)). These results show that recombinant envelope-spiked Western blots provide a simple means for serologic confirmation of STLV-I infection and that type- specific synthetic peptides can be used to confirm the virus type in seropositive monkey specimens.

Original languageEnglish (US)
Pages (from-to)858-861
Number of pages4
JournalJournal of Clinical Microbiology
Volume30
Issue number4
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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