Serologic and virologic evidence of bluetongue virus infection in cattle and sheep in Mexico.

Jeffrey L Stott, Myra Blanchard, Bennie Osburn, H. P. Riemann, R. C. Obeso

Research output: Contribution to journalArticle

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Abstract

Three independent 1-year studies were conducted during 3 consecutive years to better define the prevalence of bluetongue virus (BTV) infection in Mexico. Serologic data were obtained by use of agar-gel immunodiffusion for identification of BTV group-reactive antibodies, and virologic data were obtained by virus isolation. Samples were obtained from sheep in 6 states over a 1-year period, with 9% seropositive; samples were obtained from cattle in 11 states during the same 1-year period, with 35% seropositive. Two years later, samples were obtained from cattle in 4 additional states, with 69% seropositive. Virus isolation was conducted on pooled blood samples obtained from cattle in 7 states. Six virus isolates were recovered and included 2 isolates each of BTV serotypes 11 and 13 and 1 isolate each of serotypes 10 and 17. All virus isolates were partially characterized by electrophoretic analysis of genomic RNA migration profiles (electropherotypes) in polyacrylamide gels. All Mexican isolates of BTV differed considerably in electropherotype profile, as compared with their respective US prototype strain of the same serotype. Such differences appeared to be much more extensive than those described to exist between numerous California isolates of the same serotype.

Original languageEnglish (US)
Pages (from-to)335-340
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume50
Issue number3
StatePublished - Mar 1989

Fingerprint

Bluetongue virus
Virus Diseases
Mexico
Sheep
serotypes
Viruses
sheep
viruses
cattle
infection
gels
sampling
Immunodiffusion
polyacrylamide
prototypes
Agar
agar
Gels
RNA
genomics

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Serologic and virologic evidence of bluetongue virus infection in cattle and sheep in Mexico. / Stott, Jeffrey L; Blanchard, Myra; Osburn, Bennie; Riemann, H. P.; Obeso, R. C.

In: American Journal of Veterinary Research, Vol. 50, No. 3, 03.1989, p. 335-340.

Research output: Contribution to journalArticle

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