Serologi diagnosis of West Nile and St. Louis encephalitis virus infections in domestic chickens

Peter J. Patiris, Leopoldo F. Oceguera, George W. Peck, Robert E. Chiles, William Reisen, Carl V. Hanson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Adult domestic chickens were infected with West Nile virus (WNV) or St. Louis encephalitis virus (SLEV) and challenged with homologous or heterologous virus at 21 or 56 days postinfection (dpi). Sera were collected at selected time points after infection and assayed by enzyme immunoassay (EIA), plaque reduction neutralization test (PRNT), and a Western blot (WB) alternative to PRNT. EIA results were sensitive and accurate (few false positives) but not specific, requiring a confirmatory test to determine virus infection history. PRNT results generally were specific until challenge, after which test results were frequently equivocal and inadequate to determine first or second infecting virus. WB results confirmed the serologic cross-reactivity between WNV and SLEV envelope protein. Non-structural protein 1 and pre-membrane protein reactivities were highly specific for WNV during SLEV infection, but less specific for SLEV during WNV infection. WB and PRNT specificities were similar for both viruses from 6 to 14 dpi, and sensitivities to WNV were virtually identical.

Original languageEnglish (US)
Pages (from-to)434-441
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume78
Issue number3
StatePublished - Mar 1 2008

Fingerprint

St. Louis Encephalitis Viruses
West Nile virus
Virus Diseases
Neutralization Tests
Chickens
Western Blotting
Viruses
Immunoenzyme Techniques
Viral Envelope Proteins
Membrane Proteins
Infectious Encephalitis
Infection
Serum
Proteins

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Patiris, P. J., Oceguera, L. F., Peck, G. W., Chiles, R. E., Reisen, W., & Hanson, C. V. (2008). Serologi diagnosis of West Nile and St. Louis encephalitis virus infections in domestic chickens. American Journal of Tropical Medicine and Hygiene, 78(3), 434-441.

Serologi diagnosis of West Nile and St. Louis encephalitis virus infections in domestic chickens. / Patiris, Peter J.; Oceguera, Leopoldo F.; Peck, George W.; Chiles, Robert E.; Reisen, William; Hanson, Carl V.

In: American Journal of Tropical Medicine and Hygiene, Vol. 78, No. 3, 01.03.2008, p. 434-441.

Research output: Contribution to journalArticle

Patiris, Peter J. ; Oceguera, Leopoldo F. ; Peck, George W. ; Chiles, Robert E. ; Reisen, William ; Hanson, Carl V. / Serologi diagnosis of West Nile and St. Louis encephalitis virus infections in domestic chickens. In: American Journal of Tropical Medicine and Hygiene. 2008 ; Vol. 78, No. 3. pp. 434-441.
@article{fede776b0a2a4b929297561459689025,
title = "Serologi diagnosis of West Nile and St. Louis encephalitis virus infections in domestic chickens",
abstract = "Adult domestic chickens were infected with West Nile virus (WNV) or St. Louis encephalitis virus (SLEV) and challenged with homologous or heterologous virus at 21 or 56 days postinfection (dpi). Sera were collected at selected time points after infection and assayed by enzyme immunoassay (EIA), plaque reduction neutralization test (PRNT), and a Western blot (WB) alternative to PRNT. EIA results were sensitive and accurate (few false positives) but not specific, requiring a confirmatory test to determine virus infection history. PRNT results generally were specific until challenge, after which test results were frequently equivocal and inadequate to determine first or second infecting virus. WB results confirmed the serologic cross-reactivity between WNV and SLEV envelope protein. Non-structural protein 1 and pre-membrane protein reactivities were highly specific for WNV during SLEV infection, but less specific for SLEV during WNV infection. WB and PRNT specificities were similar for both viruses from 6 to 14 dpi, and sensitivities to WNV were virtually identical.",
author = "Patiris, {Peter J.} and Oceguera, {Leopoldo F.} and Peck, {George W.} and Chiles, {Robert E.} and William Reisen and Hanson, {Carl V.}",
year = "2008",
month = "3",
day = "1",
language = "English (US)",
volume = "78",
pages = "434--441",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "3",

}

TY - JOUR

T1 - Serologi diagnosis of West Nile and St. Louis encephalitis virus infections in domestic chickens

AU - Patiris, Peter J.

AU - Oceguera, Leopoldo F.

AU - Peck, George W.

AU - Chiles, Robert E.

AU - Reisen, William

AU - Hanson, Carl V.

PY - 2008/3/1

Y1 - 2008/3/1

N2 - Adult domestic chickens were infected with West Nile virus (WNV) or St. Louis encephalitis virus (SLEV) and challenged with homologous or heterologous virus at 21 or 56 days postinfection (dpi). Sera were collected at selected time points after infection and assayed by enzyme immunoassay (EIA), plaque reduction neutralization test (PRNT), and a Western blot (WB) alternative to PRNT. EIA results were sensitive and accurate (few false positives) but not specific, requiring a confirmatory test to determine virus infection history. PRNT results generally were specific until challenge, after which test results were frequently equivocal and inadequate to determine first or second infecting virus. WB results confirmed the serologic cross-reactivity between WNV and SLEV envelope protein. Non-structural protein 1 and pre-membrane protein reactivities were highly specific for WNV during SLEV infection, but less specific for SLEV during WNV infection. WB and PRNT specificities were similar for both viruses from 6 to 14 dpi, and sensitivities to WNV were virtually identical.

AB - Adult domestic chickens were infected with West Nile virus (WNV) or St. Louis encephalitis virus (SLEV) and challenged with homologous or heterologous virus at 21 or 56 days postinfection (dpi). Sera were collected at selected time points after infection and assayed by enzyme immunoassay (EIA), plaque reduction neutralization test (PRNT), and a Western blot (WB) alternative to PRNT. EIA results were sensitive and accurate (few false positives) but not specific, requiring a confirmatory test to determine virus infection history. PRNT results generally were specific until challenge, after which test results were frequently equivocal and inadequate to determine first or second infecting virus. WB results confirmed the serologic cross-reactivity between WNV and SLEV envelope protein. Non-structural protein 1 and pre-membrane protein reactivities were highly specific for WNV during SLEV infection, but less specific for SLEV during WNV infection. WB and PRNT specificities were similar for both viruses from 6 to 14 dpi, and sensitivities to WNV were virtually identical.

UR - http://www.scopus.com/inward/record.url?scp=42949121046&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42949121046&partnerID=8YFLogxK

M3 - Article

C2 - 18337340

AN - SCOPUS:42949121046

VL - 78

SP - 434

EP - 441

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 3

ER -