Serologi diagnosis of West Nile and St. Louis encephalitis virus infections in domestic chickens

Peter J. Patiris, Leopoldo F. Oceguera, George W. Peck, Robert E. Chiles, William Reisen, Carl V. Hanson

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Adult domestic chickens were infected with West Nile virus (WNV) or St. Louis encephalitis virus (SLEV) and challenged with homologous or heterologous virus at 21 or 56 days postinfection (dpi). Sera were collected at selected time points after infection and assayed by enzyme immunoassay (EIA), plaque reduction neutralization test (PRNT), and a Western blot (WB) alternative to PRNT. EIA results were sensitive and accurate (few false positives) but not specific, requiring a confirmatory test to determine virus infection history. PRNT results generally were specific until challenge, after which test results were frequently equivocal and inadequate to determine first or second infecting virus. WB results confirmed the serologic cross-reactivity between WNV and SLEV envelope protein. Non-structural protein 1 and pre-membrane protein reactivities were highly specific for WNV during SLEV infection, but less specific for SLEV during WNV infection. WB and PRNT specificities were similar for both viruses from 6 to 14 dpi, and sensitivities to WNV were virtually identical.

Original languageEnglish (US)
Pages (from-to)434-441
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume78
Issue number3
StatePublished - Mar 1 2008

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

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    Patiris, P. J., Oceguera, L. F., Peck, G. W., Chiles, R. E., Reisen, W., & Hanson, C. V. (2008). Serologi diagnosis of West Nile and St. Louis encephalitis virus infections in domestic chickens. American Journal of Tropical Medicine and Hygiene, 78(3), 434-441.