Abstract
The potential use of the recently reported polymerase chain reaction (PCR) protocol for detection of United States epizootic hemorrhagic disease virus (EHDV) serotype 1 (EHDV-1) and serotype 2 (EHDV-2) ribonucleic acid in cell culture and clinical specimens was evaluated for detection of Sudanese EHDV strains. EHDV serotype 5 (EHDV-5) and EHDV, isolate 318 (untyped) designated (EHDV-318), recovered from sentinel calves at the Khartoum University farm (Sudan) were studied. RNA from EHDV-5 and EHDV-318 and a number of EHDV held isolates, propagated in cell cultures, were detected by the described PCR-based assay. The specific 387 bp PCR products were visualized on ethidium-bromide stained agarose gel. Specificity of the PCR products was confirmed by chemiluminescent hybridization with non-radiolabeled internal probe. Amplification product was not detected when the PCR-based assay was applied to RNA from bluetongue virus (BTV) prototypes serotypes 2, 10, 11, 13, 16 and 17; total nucleic acid extracts from uninfected BHK-21 cells. The results of this study indicated that the previously described EHDV-PCR assay could be applied for detection of Sudanese as well as United States strains of EHDV serogroup. In addition, the described EHDV-PCR assay could be used as a supportive diagnostic assay to the current conventional virus isolation procedures used for detection of EHDV infection in susceptible ruminants.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 211-218 |
| Number of pages | 8 |
| Journal | Comparative Immunology, Microbiology and Infectious Diseases |
| Volume | 20 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 1 1997 |
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Keywords
- EHDV
- PCR
- Serogroup
- Sudanese
- Topotype
ASJC Scopus subject areas
- Microbiology
- Immunology and Allergy
- Immunology
- veterinary(all)
- Infectious Diseases
Cite this
Serogrouping and topotyping of Sudanese and United States strains of epizootic hemorrhagic disease virus using PCR. / Aradaib, Imadeldin E.; Mohammed, Mohammed E.H.; Mukhtar, Muawia M.; Ghalib, Hashim W.; Osburn, Bennie.
In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 20, No. 3, 01.06.1997, p. 211-218.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Serogrouping and topotyping of Sudanese and United States strains of epizootic hemorrhagic disease virus using PCR
AU - Aradaib, Imadeldin E.
AU - Mohammed, Mohammed E.H.
AU - Mukhtar, Muawia M.
AU - Ghalib, Hashim W.
AU - Osburn, Bennie
PY - 1997/6/1
Y1 - 1997/6/1
N2 - The potential use of the recently reported polymerase chain reaction (PCR) protocol for detection of United States epizootic hemorrhagic disease virus (EHDV) serotype 1 (EHDV-1) and serotype 2 (EHDV-2) ribonucleic acid in cell culture and clinical specimens was evaluated for detection of Sudanese EHDV strains. EHDV serotype 5 (EHDV-5) and EHDV, isolate 318 (untyped) designated (EHDV-318), recovered from sentinel calves at the Khartoum University farm (Sudan) were studied. RNA from EHDV-5 and EHDV-318 and a number of EHDV held isolates, propagated in cell cultures, were detected by the described PCR-based assay. The specific 387 bp PCR products were visualized on ethidium-bromide stained agarose gel. Specificity of the PCR products was confirmed by chemiluminescent hybridization with non-radiolabeled internal probe. Amplification product was not detected when the PCR-based assay was applied to RNA from bluetongue virus (BTV) prototypes serotypes 2, 10, 11, 13, 16 and 17; total nucleic acid extracts from uninfected BHK-21 cells. The results of this study indicated that the previously described EHDV-PCR assay could be applied for detection of Sudanese as well as United States strains of EHDV serogroup. In addition, the described EHDV-PCR assay could be used as a supportive diagnostic assay to the current conventional virus isolation procedures used for detection of EHDV infection in susceptible ruminants.
AB - The potential use of the recently reported polymerase chain reaction (PCR) protocol for detection of United States epizootic hemorrhagic disease virus (EHDV) serotype 1 (EHDV-1) and serotype 2 (EHDV-2) ribonucleic acid in cell culture and clinical specimens was evaluated for detection of Sudanese EHDV strains. EHDV serotype 5 (EHDV-5) and EHDV, isolate 318 (untyped) designated (EHDV-318), recovered from sentinel calves at the Khartoum University farm (Sudan) were studied. RNA from EHDV-5 and EHDV-318 and a number of EHDV held isolates, propagated in cell cultures, were detected by the described PCR-based assay. The specific 387 bp PCR products were visualized on ethidium-bromide stained agarose gel. Specificity of the PCR products was confirmed by chemiluminescent hybridization with non-radiolabeled internal probe. Amplification product was not detected when the PCR-based assay was applied to RNA from bluetongue virus (BTV) prototypes serotypes 2, 10, 11, 13, 16 and 17; total nucleic acid extracts from uninfected BHK-21 cells. The results of this study indicated that the previously described EHDV-PCR assay could be applied for detection of Sudanese as well as United States strains of EHDV serogroup. In addition, the described EHDV-PCR assay could be used as a supportive diagnostic assay to the current conventional virus isolation procedures used for detection of EHDV infection in susceptible ruminants.
KW - EHDV
KW - PCR
KW - Serogroup
KW - Sudanese
KW - Topotype
UR - http://www.scopus.com/inward/record.url?scp=0030765379&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030765379&partnerID=8YFLogxK
U2 - 10.1016/S0147-9571(97)00002-7
DO - 10.1016/S0147-9571(97)00002-7
M3 - Article
C2 - 9280388
AN - SCOPUS:0030765379
VL - 20
SP - 211
EP - 218
JO - Comparative Immunology, Microbiology and Infectious Diseases
JF - Comparative Immunology, Microbiology and Infectious Diseases
SN - 0147-9571
IS - 3
ER -