Seroepidemiologic survey of captive old-world primates for antibodies to human and simian retroviruses, and isolation of a lentivirus from sooty mangabeys (Cercocebus atys)

Linda J Lowenstine, Niels C Pedersen, J. Higgins, K. C. Pallis, A. Uyeda, P. Marx, N. W. Lerche, R. J. Munn, M. B. Gardner

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Abstract

Sera from 526 Old-World monkeys and apes, representing 50 species and 20 genera and living in US zoos and vivaria, were screened for antibodies to HTLV-I, HTLV-III/LAV, and simian-AIDS retrovirus, type I (SRV-I). Sera were screened initially by ELISA, and ELISA-positive sera, as well as ELISA-negative sera from cage contacts, were further tested by Western blotting. A large number of false-positive and a small number of false-negative ELISA sera were identified. Although most true positive reactions were directed to a single retrovirus, a number of individuals from 4 species were positive for more than one retrovirus. Specific seroreactivity to HTLV-I was found in 39/526 (7%) animals of 15 species. True positive reactions to SRV-I were found in 21/516 (4%) animals, including talapoins and 2 species of macaques. Specific serologic reactions to HTLV-III/LAV were detected in 23/526 (4%) monkeys. Many of the HTLV-III/LAV seropositive animals were from one mixed-species zoo exhibit, containing sooty mangabeys, mandrills, Kolb's guenons, and talapoins. A type D virus was isolated from the blood of 3/10 SRV-I antibody-positive Tonkeana macaques, but from none of 11 seropositive talapoins. A lentivirus was isolated from the blood of 4/7 HTLV-III/LAV seropositive sooty mangabeys, but not from seropositive talapoins in the same exhibit or from 2 seropositive colobus from another zoo. The sooty mangabey lentivirus produced generalized lymphadenopathy, leukopenia, and decreased levels of T4 lymphocytes in 2 experimentally infected rhesus macaques.

Original languageEnglish (US)
Pages (from-to)563-574
Number of pages12
JournalInternational Journal of Cancer
Volume38
Issue number4
StatePublished - 1986

Fingerprint

Simian Retroviruses
Cercocebus atys
Lentivirus
Simian Acquired Immunodeficiency Syndrome
Primates
Enzyme-Linked Immunosorbent Assay
Antibodies
HIV
Serum
Macaca
Retroviridae
Colobus
HTLV-I Antibodies
Far-Western Blotting
Cercopithecidae
CD4-Positive T-Lymphocytes
Mandrillus
Human T-lymphotropic virus 1
Hominidae
Leukopenia

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Seroepidemiologic survey of captive old-world primates for antibodies to human and simian retroviruses, and isolation of a lentivirus from sooty mangabeys (Cercocebus atys). / Lowenstine, Linda J; Pedersen, Niels C; Higgins, J.; Pallis, K. C.; Uyeda, A.; Marx, P.; Lerche, N. W.; Munn, R. J.; Gardner, M. B.

In: International Journal of Cancer, Vol. 38, No. 4, 1986, p. 563-574.

Research output: Contribution to journalArticle

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abstract = "Sera from 526 Old-World monkeys and apes, representing 50 species and 20 genera and living in US zoos and vivaria, were screened for antibodies to HTLV-I, HTLV-III/LAV, and simian-AIDS retrovirus, type I (SRV-I). Sera were screened initially by ELISA, and ELISA-positive sera, as well as ELISA-negative sera from cage contacts, were further tested by Western blotting. A large number of false-positive and a small number of false-negative ELISA sera were identified. Although most true positive reactions were directed to a single retrovirus, a number of individuals from 4 species were positive for more than one retrovirus. Specific seroreactivity to HTLV-I was found in 39/526 (7{\%}) animals of 15 species. True positive reactions to SRV-I were found in 21/516 (4{\%}) animals, including talapoins and 2 species of macaques. Specific serologic reactions to HTLV-III/LAV were detected in 23/526 (4{\%}) monkeys. Many of the HTLV-III/LAV seropositive animals were from one mixed-species zoo exhibit, containing sooty mangabeys, mandrills, Kolb's guenons, and talapoins. A type D virus was isolated from the blood of 3/10 SRV-I antibody-positive Tonkeana macaques, but from none of 11 seropositive talapoins. A lentivirus was isolated from the blood of 4/7 HTLV-III/LAV seropositive sooty mangabeys, but not from seropositive talapoins in the same exhibit or from 2 seropositive colobus from another zoo. The sooty mangabey lentivirus produced generalized lymphadenopathy, leukopenia, and decreased levels of T4 lymphocytes in 2 experimentally infected rhesus macaques.",
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AU - Higgins, J.

AU - Pallis, K. C.

AU - Uyeda, A.

AU - Marx, P.

AU - Lerche, N. W.

AU - Munn, R. J.

AU - Gardner, M. B.

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