Sera from patients with chronic Lyme disease protect mice from Lyme borreliosis

Erol Fikrig, Linda K. Bockenstedt, Stephen W Barthold, Manchuan Chen, Hong Tao, Pia Ali-Salaam, Sam R. Telford, Richard A. Flavell

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Sera from selected patients with Lyme disease in different stages were used to passively immunize mice against Borrelia burgdorferi challenge to determine if human antibodies could protect the animals from infection. Sera from 2 patients with late-stage Lyme disease that contained strong antibody reactivity to proteins in B. burgdorferi lysates, including antibodies to the outer surface proteins (Osps) A and B, partly protected mice from infection after challenge with a small inoculum (102) of B. burgdorferi. Mice immunized with sera from either of these 2 patients developed significantly fewer infections from the borreliae (patient 1 serum, 5%; patient 2 serum, 25%) relative to control mice (patient 1 serum, 90%; patient 2 serum, 74%). In contrast, sera from 2 patients with early or late Lyme disease that lacked antibodies reactive to OspA and OspB did not confer protection. Immunity appeared to be related, at least in part, to the presence of a strong humoral response to the Osps. These results suggest that during prolonged infection, some patients develop an immune response that may be partly protective against reinfection with B. burgdorferi. Therefore, although most patients do not mount a strong humoral response to the Osps during natural infection, vaccination with an Osp may elicit protective immunity.

Original languageEnglish (US)
Pages (from-to)568-574
Number of pages7
JournalJournal of Infectious Diseases
Volume169
Issue number3
StatePublished - Mar 1994
Externally publishedYes

Fingerprint

Lyme Disease
Chronic Disease
Serum
Borrelia burgdorferi
Antibodies
Infection
Immunity
Membrane Proteins
Borrelia Infections
Vaccination

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Fikrig, E., Bockenstedt, L. K., Barthold, S. W., Chen, M., Tao, H., Ali-Salaam, P., ... Flavell, R. A. (1994). Sera from patients with chronic Lyme disease protect mice from Lyme borreliosis. Journal of Infectious Diseases, 169(3), 568-574.

Sera from patients with chronic Lyme disease protect mice from Lyme borreliosis. / Fikrig, Erol; Bockenstedt, Linda K.; Barthold, Stephen W; Chen, Manchuan; Tao, Hong; Ali-Salaam, Pia; Telford, Sam R.; Flavell, Richard A.

In: Journal of Infectious Diseases, Vol. 169, No. 3, 03.1994, p. 568-574.

Research output: Contribution to journalArticle

Fikrig, E, Bockenstedt, LK, Barthold, SW, Chen, M, Tao, H, Ali-Salaam, P, Telford, SR & Flavell, RA 1994, 'Sera from patients with chronic Lyme disease protect mice from Lyme borreliosis', Journal of Infectious Diseases, vol. 169, no. 3, pp. 568-574.
Fikrig E, Bockenstedt LK, Barthold SW, Chen M, Tao H, Ali-Salaam P et al. Sera from patients with chronic Lyme disease protect mice from Lyme borreliosis. Journal of Infectious Diseases. 1994 Mar;169(3):568-574.
Fikrig, Erol ; Bockenstedt, Linda K. ; Barthold, Stephen W ; Chen, Manchuan ; Tao, Hong ; Ali-Salaam, Pia ; Telford, Sam R. ; Flavell, Richard A. / Sera from patients with chronic Lyme disease protect mice from Lyme borreliosis. In: Journal of Infectious Diseases. 1994 ; Vol. 169, No. 3. pp. 568-574.
@article{3f91b7d6f00f4a9ca15a1f7ec66a22e5,
title = "Sera from patients with chronic Lyme disease protect mice from Lyme borreliosis",
abstract = "Sera from selected patients with Lyme disease in different stages were used to passively immunize mice against Borrelia burgdorferi challenge to determine if human antibodies could protect the animals from infection. Sera from 2 patients with late-stage Lyme disease that contained strong antibody reactivity to proteins in B. burgdorferi lysates, including antibodies to the outer surface proteins (Osps) A and B, partly protected mice from infection after challenge with a small inoculum (102) of B. burgdorferi. Mice immunized with sera from either of these 2 patients developed significantly fewer infections from the borreliae (patient 1 serum, 5{\%}; patient 2 serum, 25{\%}) relative to control mice (patient 1 serum, 90{\%}; patient 2 serum, 74{\%}). In contrast, sera from 2 patients with early or late Lyme disease that lacked antibodies reactive to OspA and OspB did not confer protection. Immunity appeared to be related, at least in part, to the presence of a strong humoral response to the Osps. These results suggest that during prolonged infection, some patients develop an immune response that may be partly protective against reinfection with B. burgdorferi. Therefore, although most patients do not mount a strong humoral response to the Osps during natural infection, vaccination with an Osp may elicit protective immunity.",
author = "Erol Fikrig and Bockenstedt, {Linda K.} and Barthold, {Stephen W} and Manchuan Chen and Hong Tao and Pia Ali-Salaam and Telford, {Sam R.} and Flavell, {Richard A.}",
year = "1994",
month = "3",
language = "English (US)",
volume = "169",
pages = "568--574",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "3",

}

TY - JOUR

T1 - Sera from patients with chronic Lyme disease protect mice from Lyme borreliosis

AU - Fikrig, Erol

AU - Bockenstedt, Linda K.

AU - Barthold, Stephen W

AU - Chen, Manchuan

AU - Tao, Hong

AU - Ali-Salaam, Pia

AU - Telford, Sam R.

AU - Flavell, Richard A.

PY - 1994/3

Y1 - 1994/3

N2 - Sera from selected patients with Lyme disease in different stages were used to passively immunize mice against Borrelia burgdorferi challenge to determine if human antibodies could protect the animals from infection. Sera from 2 patients with late-stage Lyme disease that contained strong antibody reactivity to proteins in B. burgdorferi lysates, including antibodies to the outer surface proteins (Osps) A and B, partly protected mice from infection after challenge with a small inoculum (102) of B. burgdorferi. Mice immunized with sera from either of these 2 patients developed significantly fewer infections from the borreliae (patient 1 serum, 5%; patient 2 serum, 25%) relative to control mice (patient 1 serum, 90%; patient 2 serum, 74%). In contrast, sera from 2 patients with early or late Lyme disease that lacked antibodies reactive to OspA and OspB did not confer protection. Immunity appeared to be related, at least in part, to the presence of a strong humoral response to the Osps. These results suggest that during prolonged infection, some patients develop an immune response that may be partly protective against reinfection with B. burgdorferi. Therefore, although most patients do not mount a strong humoral response to the Osps during natural infection, vaccination with an Osp may elicit protective immunity.

AB - Sera from selected patients with Lyme disease in different stages were used to passively immunize mice against Borrelia burgdorferi challenge to determine if human antibodies could protect the animals from infection. Sera from 2 patients with late-stage Lyme disease that contained strong antibody reactivity to proteins in B. burgdorferi lysates, including antibodies to the outer surface proteins (Osps) A and B, partly protected mice from infection after challenge with a small inoculum (102) of B. burgdorferi. Mice immunized with sera from either of these 2 patients developed significantly fewer infections from the borreliae (patient 1 serum, 5%; patient 2 serum, 25%) relative to control mice (patient 1 serum, 90%; patient 2 serum, 74%). In contrast, sera from 2 patients with early or late Lyme disease that lacked antibodies reactive to OspA and OspB did not confer protection. Immunity appeared to be related, at least in part, to the presence of a strong humoral response to the Osps. These results suggest that during prolonged infection, some patients develop an immune response that may be partly protective against reinfection with B. burgdorferi. Therefore, although most patients do not mount a strong humoral response to the Osps during natural infection, vaccination with an Osp may elicit protective immunity.

UR - http://www.scopus.com/inward/record.url?scp=0028008686&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028008686&partnerID=8YFLogxK

M3 - Article

VL - 169

SP - 568

EP - 574

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 3

ER -