Sequential one-pot multienzyme (OPME) synthesis of lacto-N-neotetraose and its sialyl and fucosyl derivatives

Congcong Chen, Yan Zhang, Mengyang Xue, Xian Wei Liu, Yanhong Li, Xi Chen, Peng George Wang, Fengshan Wang, Hongzhi Cao

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Lacto-N-neotetraose and its sialyl and fucosyl derivatives including Lewis x (Le<sup>x</sup>) pentasaccharide, sialyl Lewis x (sLe<sup>x</sup>) hexasaccharide and internally sialylated derivatives were enzymatically synthesized from readily available lactoside, commercially available uridine 5′-diphosphate-glucose (UDP-Glc) and the corresponding monosaccharides using a highly efficient sequential one-pot multienzyme (OPME) strategy. The OPME strategy which combines bacterial glycosyltransferases and sugar nucleotide generation enzymes provides easy access to the biologically important complex oligosaccharides at preparative scale. Moreover, the same OPME strategy can be used for the regioselective introduction of sialic acid to the internal galactose unit of LNnT in a designed glycosylation route by simply changing the glycosylation sequence.

Original languageEnglish (US)
Pages (from-to)7689-7692
Number of pages4
JournalChemical Communications
Volume51
Issue number36
DOIs
StatePublished - May 4 2015

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Metals and Alloys

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