Abstract
The cardiac action potential (AP) is shaped by myriad ionic currents. In this study, we develop an innovative AP-clamp Sequential Dissection technique to enable the recording of multiple ionic currents in the single cell under AP-clamp. This new technique presents a significant step beyond the traditional way of recording only one current in any one cell. The ability to measure many currents in a single cell has revealed two hitherto unknown characteristics of the ionic currents in cardiac cells: coordination of currents within a cell and large variation of currents between cells. Hence, the AP-clamp Sequential Dissection method provides a unique and powerful tool for studying individual cell electrophysiology.
Original language | English (US) |
---|---|
Pages (from-to) | 578-581 |
Number of pages | 4 |
Journal | Journal of Molecular and Cellular Cardiology |
Volume | 50 |
Issue number | 3 |
DOIs | |
State | Published - Mar 2011 |
Keywords
- Action potential
- AP-clamp
- Arrhythmia
- Ca channel
- Cardiac
- K channel
ASJC Scopus subject areas
- Molecular Biology
- Cardiology and Cardiovascular Medicine