Sequential dissection of multiple ionic currents in single cardiac myocytes under action potential-clamp

Tamas Banyasz, Balazs Horvath, Zhong Jian, Leighton T Izu, Ye Chen-Izu

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The cardiac action potential (AP) is shaped by myriad ionic currents. In this study, we develop an innovative AP-clamp Sequential Dissection technique to enable the recording of multiple ionic currents in the single cell under AP-clamp. This new technique presents a significant step beyond the traditional way of recording only one current in any one cell. The ability to measure many currents in a single cell has revealed two hitherto unknown characteristics of the ionic currents in cardiac cells: coordination of currents within a cell and large variation of currents between cells. Hence, the AP-clamp Sequential Dissection method provides a unique and powerful tool for studying individual cell electrophysiology.

Original languageEnglish (US)
Pages (from-to)578-581
Number of pages4
JournalJournal of Molecular and Cellular Cardiology
Volume50
Issue number3
DOIs
StatePublished - Mar 2011

Keywords

  • Action potential
  • AP-clamp
  • Arrhythmia
  • Ca channel
  • Cardiac
  • K channel

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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