Sequencing of seven haloarchaeal genomes reveals patterns of genomic flux

Erin A. Lynch, Morgan G I Langille, Aaron Darling, Elizabeth G. Wilbanks, Caitlin Haltiner, Katie S Y Shao, Michael O. Starr, Clotilde Teiling, Timothy T. Harkins, Robert A. Edwards, Jonathan A Eisen, Marc T. Facciotti

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We report the sequencing of seven genomes from two haloarchaeal genera, Haloferax and Haloarcula. Ease of cultivation and the existence of well-developed genetic and biochemical tools for several diverse haloarchaeal species make haloarchaea a model group for the study of archaeal biology. The unique physiological properties of these organisms also make them good candidates for novel enzyme discovery for biotechnological applications. Seven genomes were sequenced to ~20×coverage and assembled to an average of 50 contigs (range 5 scaffolds - 168 contigs). Comparisons of protein-coding gene compliments revealed large-scale differences in COG functional group enrichment between these genera. Analysis of genes encoding machinery for DNA metabolism reveals genera-specific expansions of the general transcription factor TATA binding protein as well as a history of extensive duplication and horizontal transfer of the proliferating cell nuclear antigen. Insights gained from this study emphasize the importance of haloarchaea for investigation of archaeal biology.

Original languageEnglish (US)
Article numbere41389
JournalPLoS One
Volume7
Issue number7
DOIs
StatePublished - Jul 24 2012

Fingerprint

Haloferax
Haloarcula
Genes
General Transcription Factors
Genome
TATA-Box Binding Protein
Fluxes
genomics
Biological Sciences
proliferating cell nuclear antigen
genome
Proliferating Cell Nuclear Antigen
binding proteins
Molecular Biology
Gene encoding
genes
transcription factors
Metabolism
Scaffolds
Functional groups

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lynch, E. A., Langille, M. G. I., Darling, A., Wilbanks, E. G., Haltiner, C., Shao, K. S. Y., ... Facciotti, M. T. (2012). Sequencing of seven haloarchaeal genomes reveals patterns of genomic flux. PLoS One, 7(7), [e41389]. https://doi.org/10.1371/journal.pone.0041389

Sequencing of seven haloarchaeal genomes reveals patterns of genomic flux. / Lynch, Erin A.; Langille, Morgan G I; Darling, Aaron; Wilbanks, Elizabeth G.; Haltiner, Caitlin; Shao, Katie S Y; Starr, Michael O.; Teiling, Clotilde; Harkins, Timothy T.; Edwards, Robert A.; Eisen, Jonathan A; Facciotti, Marc T.

In: PLoS One, Vol. 7, No. 7, e41389, 24.07.2012.

Research output: Contribution to journalArticle

Lynch, EA, Langille, MGI, Darling, A, Wilbanks, EG, Haltiner, C, Shao, KSY, Starr, MO, Teiling, C, Harkins, TT, Edwards, RA, Eisen, JA & Facciotti, MT 2012, 'Sequencing of seven haloarchaeal genomes reveals patterns of genomic flux', PLoS One, vol. 7, no. 7, e41389. https://doi.org/10.1371/journal.pone.0041389
Lynch EA, Langille MGI, Darling A, Wilbanks EG, Haltiner C, Shao KSY et al. Sequencing of seven haloarchaeal genomes reveals patterns of genomic flux. PLoS One. 2012 Jul 24;7(7). e41389. https://doi.org/10.1371/journal.pone.0041389
Lynch, Erin A. ; Langille, Morgan G I ; Darling, Aaron ; Wilbanks, Elizabeth G. ; Haltiner, Caitlin ; Shao, Katie S Y ; Starr, Michael O. ; Teiling, Clotilde ; Harkins, Timothy T. ; Edwards, Robert A. ; Eisen, Jonathan A ; Facciotti, Marc T. / Sequencing of seven haloarchaeal genomes reveals patterns of genomic flux. In: PLoS One. 2012 ; Vol. 7, No. 7.
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