Sequencing and analysis of a 63 kb bacterial artificial chromosome insert from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi

Jennifer Ware, Laurie Moran, Jeremy Foster, Janos Posfai, Tamas Vincze, David Guiliano, Mark Blaxter, Jonathan A Eisen, Barton Slatko

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Wolbachia endosymbiotic bacteria are widespread in filarial nematodes and are directly involved in the immune response of the host. In addition, antibiotics which disrupt Wolbachia interfere with filarial nematode development thus, Wolbachia provide an excellent target for control of filariasis. A 63.1 kb bacterial artificial chromosome insert, from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi, has been sequenced using the New England Biolabs Inc. Genome Priming System™ transposition kit in conjunction with primer walking methods. The bacterial artificial chromosome insert contains approximately 57 potential ORFs which have been compared by individual protein BLAST analysis with the 35 published complete microbial genomes in the Comprehensive Microbial Resource database at The Institute for Genomic Research and in the NCBI GenBank database, as well as to data from 22 incomplete genomes from the DOE Joint Genome Institute. Twenty five of the putative ORFs have significant similarity to genes from the α-proteobacteria Rickettsia prowazekii, the most closely related completed genome, as well as to the newly sequenced α-proteobacteria endosymbiont Sinorhizobium meliloti. The bacterial artificial chromosome insert sequence however has little conserved synteny with the R. prowazekii and S. meliloti genomes. Significant sequence similarity was also found in comparisons with the currently available sequence data from the Wolbachia endosymbiont of Drosophila melanogaster. Analysis of this bacterial artificial chromosome insert provides useful gene density and comparative genomic data that will contribute to whole genome sequencing of Wolbachia from the B. malayi host. This will also lead to a better understanding of the interactions between the endosymbiont and its host and will offer novel approaches and drug targets for elimination of filarial disease.

Original languageEnglish (US)
Pages (from-to)159-166
Number of pages8
JournalInternational Journal for Parasitology
Volume32
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Brugia malayi
Wolbachia
Bacterial Artificial Chromosomes
Parasites
Genome
Rickettsia prowazekii
Sinorhizobium meliloti
Proteobacteria
Open Reading Frames
Microbial Genome
Databases
Disease Eradication
Synteny
Filariasis
New England
Nucleic Acid Databases
Drosophila melanogaster
Genes
Walking
Joints

Keywords

  • BAC
  • Brugia malayi
  • DNA sequencing
  • Filarial parasite
  • Transposon
  • Wolbachia

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Sequencing and analysis of a 63 kb bacterial artificial chromosome insert from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi. / Ware, Jennifer; Moran, Laurie; Foster, Jeremy; Posfai, Janos; Vincze, Tamas; Guiliano, David; Blaxter, Mark; Eisen, Jonathan A; Slatko, Barton.

In: International Journal for Parasitology, Vol. 32, No. 2, 2002, p. 159-166.

Research output: Contribution to journalArticle

Ware, Jennifer ; Moran, Laurie ; Foster, Jeremy ; Posfai, Janos ; Vincze, Tamas ; Guiliano, David ; Blaxter, Mark ; Eisen, Jonathan A ; Slatko, Barton. / Sequencing and analysis of a 63 kb bacterial artificial chromosome insert from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi. In: International Journal for Parasitology. 2002 ; Vol. 32, No. 2. pp. 159-166.
@article{e0eeb905a6554168b502af255e005186,
title = "Sequencing and analysis of a 63 kb bacterial artificial chromosome insert from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi",
abstract = "Wolbachia endosymbiotic bacteria are widespread in filarial nematodes and are directly involved in the immune response of the host. In addition, antibiotics which disrupt Wolbachia interfere with filarial nematode development thus, Wolbachia provide an excellent target for control of filariasis. A 63.1 kb bacterial artificial chromosome insert, from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi, has been sequenced using the New England Biolabs Inc. Genome Priming System™ transposition kit in conjunction with primer walking methods. The bacterial artificial chromosome insert contains approximately 57 potential ORFs which have been compared by individual protein BLAST analysis with the 35 published complete microbial genomes in the Comprehensive Microbial Resource database at The Institute for Genomic Research and in the NCBI GenBank database, as well as to data from 22 incomplete genomes from the DOE Joint Genome Institute. Twenty five of the putative ORFs have significant similarity to genes from the α-proteobacteria Rickettsia prowazekii, the most closely related completed genome, as well as to the newly sequenced α-proteobacteria endosymbiont Sinorhizobium meliloti. The bacterial artificial chromosome insert sequence however has little conserved synteny with the R. prowazekii and S. meliloti genomes. Significant sequence similarity was also found in comparisons with the currently available sequence data from the Wolbachia endosymbiont of Drosophila melanogaster. Analysis of this bacterial artificial chromosome insert provides useful gene density and comparative genomic data that will contribute to whole genome sequencing of Wolbachia from the B. malayi host. This will also lead to a better understanding of the interactions between the endosymbiont and its host and will offer novel approaches and drug targets for elimination of filarial disease.",
keywords = "BAC, Brugia malayi, DNA sequencing, Filarial parasite, Transposon, Wolbachia",
author = "Jennifer Ware and Laurie Moran and Jeremy Foster and Janos Posfai and Tamas Vincze and David Guiliano and Mark Blaxter and Eisen, {Jonathan A} and Barton Slatko",
year = "2002",
doi = "10.1016/S0020-7519(01)00367-8",
language = "English (US)",
volume = "32",
pages = "159--166",
journal = "International Journal for Parasitology",
issn = "0020-7519",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Sequencing and analysis of a 63 kb bacterial artificial chromosome insert from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi

AU - Ware, Jennifer

AU - Moran, Laurie

AU - Foster, Jeremy

AU - Posfai, Janos

AU - Vincze, Tamas

AU - Guiliano, David

AU - Blaxter, Mark

AU - Eisen, Jonathan A

AU - Slatko, Barton

PY - 2002

Y1 - 2002

N2 - Wolbachia endosymbiotic bacteria are widespread in filarial nematodes and are directly involved in the immune response of the host. In addition, antibiotics which disrupt Wolbachia interfere with filarial nematode development thus, Wolbachia provide an excellent target for control of filariasis. A 63.1 kb bacterial artificial chromosome insert, from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi, has been sequenced using the New England Biolabs Inc. Genome Priming System™ transposition kit in conjunction with primer walking methods. The bacterial artificial chromosome insert contains approximately 57 potential ORFs which have been compared by individual protein BLAST analysis with the 35 published complete microbial genomes in the Comprehensive Microbial Resource database at The Institute for Genomic Research and in the NCBI GenBank database, as well as to data from 22 incomplete genomes from the DOE Joint Genome Institute. Twenty five of the putative ORFs have significant similarity to genes from the α-proteobacteria Rickettsia prowazekii, the most closely related completed genome, as well as to the newly sequenced α-proteobacteria endosymbiont Sinorhizobium meliloti. The bacterial artificial chromosome insert sequence however has little conserved synteny with the R. prowazekii and S. meliloti genomes. Significant sequence similarity was also found in comparisons with the currently available sequence data from the Wolbachia endosymbiont of Drosophila melanogaster. Analysis of this bacterial artificial chromosome insert provides useful gene density and comparative genomic data that will contribute to whole genome sequencing of Wolbachia from the B. malayi host. This will also lead to a better understanding of the interactions between the endosymbiont and its host and will offer novel approaches and drug targets for elimination of filarial disease.

AB - Wolbachia endosymbiotic bacteria are widespread in filarial nematodes and are directly involved in the immune response of the host. In addition, antibiotics which disrupt Wolbachia interfere with filarial nematode development thus, Wolbachia provide an excellent target for control of filariasis. A 63.1 kb bacterial artificial chromosome insert, from the Wolbachia endosymbiont of the human filarial parasite Brugia malayi, has been sequenced using the New England Biolabs Inc. Genome Priming System™ transposition kit in conjunction with primer walking methods. The bacterial artificial chromosome insert contains approximately 57 potential ORFs which have been compared by individual protein BLAST analysis with the 35 published complete microbial genomes in the Comprehensive Microbial Resource database at The Institute for Genomic Research and in the NCBI GenBank database, as well as to data from 22 incomplete genomes from the DOE Joint Genome Institute. Twenty five of the putative ORFs have significant similarity to genes from the α-proteobacteria Rickettsia prowazekii, the most closely related completed genome, as well as to the newly sequenced α-proteobacteria endosymbiont Sinorhizobium meliloti. The bacterial artificial chromosome insert sequence however has little conserved synteny with the R. prowazekii and S. meliloti genomes. Significant sequence similarity was also found in comparisons with the currently available sequence data from the Wolbachia endosymbiont of Drosophila melanogaster. Analysis of this bacterial artificial chromosome insert provides useful gene density and comparative genomic data that will contribute to whole genome sequencing of Wolbachia from the B. malayi host. This will also lead to a better understanding of the interactions between the endosymbiont and its host and will offer novel approaches and drug targets for elimination of filarial disease.

KW - BAC

KW - Brugia malayi

KW - DNA sequencing

KW - Filarial parasite

KW - Transposon

KW - Wolbachia

UR - http://www.scopus.com/inward/record.url?scp=0036151219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036151219&partnerID=8YFLogxK

U2 - 10.1016/S0020-7519(01)00367-8

DO - 10.1016/S0020-7519(01)00367-8

M3 - Article

C2 - 11812492

AN - SCOPUS:0036151219

VL - 32

SP - 159

EP - 166

JO - International Journal for Parasitology

JF - International Journal for Parasitology

SN - 0020-7519

IS - 2

ER -