Sequences of sea urchin kinesin light chain isoforms

Karen P. Wedaman, Alex E. Knight, John Kendrick-Jones, Jonathan M. Scholey

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We have deduced the amino acid sequences of four sea urchin (Strongylocentrotus purpuratus; SP) kinesin light chain (KLC) isoforms (SPKLC 1-4) and compared them to rat brain light chain sequences. Examination of the SPKLC open reading frames (SPKLC1, 649; SPKLC2, 677; SPKLC3, 686; and SPKLC4, 451 amino acid residues) reveals that the first 500 or so residues of the KLCs are highly conserved but the C-terminal ends of rat and sea urchin light chains are divergent; SPKLCs 1, 2 and 3 share a highly basic, 86 residue C-terminal segment that is missing from the shorter rat light chains and SPKLC4. The insertion of 28 and 37 residue segments at residue 563 of SPKLCs 2 and 3, respectively, gives rise to sequence heterogeneity at the C-terminal ends of the sea urchin KLCs. C-terminal sequence differences between light chains may provide inter- and intraspecies differences in the functional properties of the presumptive cargo attachment elements of kinesin.

Original languageEnglish (US)
Pages (from-to)155-158
Number of pages4
JournalJournal of Molecular Biology
Volume231
Issue number1
StatePublished - 1993

Fingerprint

Kinesin
Sea Urchins
Protein Isoforms
Light
Strongylocentrotus purpuratus
Open Reading Frames
Amino Acid Sequence
Amino Acids
Brain

Keywords

  • Kinesin
  • Light chain sequences
  • Organelle transport

ASJC Scopus subject areas

  • Virology

Cite this

Wedaman, K. P., Knight, A. E., Kendrick-Jones, J., & Scholey, J. M. (1993). Sequences of sea urchin kinesin light chain isoforms. Journal of Molecular Biology, 231(1), 155-158.

Sequences of sea urchin kinesin light chain isoforms. / Wedaman, Karen P.; Knight, Alex E.; Kendrick-Jones, John; Scholey, Jonathan M.

In: Journal of Molecular Biology, Vol. 231, No. 1, 1993, p. 155-158.

Research output: Contribution to journalArticle

Wedaman, KP, Knight, AE, Kendrick-Jones, J & Scholey, JM 1993, 'Sequences of sea urchin kinesin light chain isoforms', Journal of Molecular Biology, vol. 231, no. 1, pp. 155-158.
Wedaman KP, Knight AE, Kendrick-Jones J, Scholey JM. Sequences of sea urchin kinesin light chain isoforms. Journal of Molecular Biology. 1993;231(1):155-158.
Wedaman, Karen P. ; Knight, Alex E. ; Kendrick-Jones, John ; Scholey, Jonathan M. / Sequences of sea urchin kinesin light chain isoforms. In: Journal of Molecular Biology. 1993 ; Vol. 231, No. 1. pp. 155-158.
@article{61e2a6048f0a42c1aa85f5a8451c6939,
title = "Sequences of sea urchin kinesin light chain isoforms",
abstract = "We have deduced the amino acid sequences of four sea urchin (Strongylocentrotus purpuratus; SP) kinesin light chain (KLC) isoforms (SPKLC 1-4) and compared them to rat brain light chain sequences. Examination of the SPKLC open reading frames (SPKLC1, 649; SPKLC2, 677; SPKLC3, 686; and SPKLC4, 451 amino acid residues) reveals that the first 500 or so residues of the KLCs are highly conserved but the C-terminal ends of rat and sea urchin light chains are divergent; SPKLCs 1, 2 and 3 share a highly basic, 86 residue C-terminal segment that is missing from the shorter rat light chains and SPKLC4. The insertion of 28 and 37 residue segments at residue 563 of SPKLCs 2 and 3, respectively, gives rise to sequence heterogeneity at the C-terminal ends of the sea urchin KLCs. C-terminal sequence differences between light chains may provide inter- and intraspecies differences in the functional properties of the presumptive cargo attachment elements of kinesin.",
keywords = "Kinesin, Light chain sequences, Organelle transport",
author = "Wedaman, {Karen P.} and Knight, {Alex E.} and John Kendrick-Jones and Scholey, {Jonathan M.}",
year = "1993",
language = "English (US)",
volume = "231",
pages = "155--158",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Sequences of sea urchin kinesin light chain isoforms

AU - Wedaman, Karen P.

AU - Knight, Alex E.

AU - Kendrick-Jones, John

AU - Scholey, Jonathan M.

PY - 1993

Y1 - 1993

N2 - We have deduced the amino acid sequences of four sea urchin (Strongylocentrotus purpuratus; SP) kinesin light chain (KLC) isoforms (SPKLC 1-4) and compared them to rat brain light chain sequences. Examination of the SPKLC open reading frames (SPKLC1, 649; SPKLC2, 677; SPKLC3, 686; and SPKLC4, 451 amino acid residues) reveals that the first 500 or so residues of the KLCs are highly conserved but the C-terminal ends of rat and sea urchin light chains are divergent; SPKLCs 1, 2 and 3 share a highly basic, 86 residue C-terminal segment that is missing from the shorter rat light chains and SPKLC4. The insertion of 28 and 37 residue segments at residue 563 of SPKLCs 2 and 3, respectively, gives rise to sequence heterogeneity at the C-terminal ends of the sea urchin KLCs. C-terminal sequence differences between light chains may provide inter- and intraspecies differences in the functional properties of the presumptive cargo attachment elements of kinesin.

AB - We have deduced the amino acid sequences of four sea urchin (Strongylocentrotus purpuratus; SP) kinesin light chain (KLC) isoforms (SPKLC 1-4) and compared them to rat brain light chain sequences. Examination of the SPKLC open reading frames (SPKLC1, 649; SPKLC2, 677; SPKLC3, 686; and SPKLC4, 451 amino acid residues) reveals that the first 500 or so residues of the KLCs are highly conserved but the C-terminal ends of rat and sea urchin light chains are divergent; SPKLCs 1, 2 and 3 share a highly basic, 86 residue C-terminal segment that is missing from the shorter rat light chains and SPKLC4. The insertion of 28 and 37 residue segments at residue 563 of SPKLCs 2 and 3, respectively, gives rise to sequence heterogeneity at the C-terminal ends of the sea urchin KLCs. C-terminal sequence differences between light chains may provide inter- and intraspecies differences in the functional properties of the presumptive cargo attachment elements of kinesin.

KW - Kinesin

KW - Light chain sequences

KW - Organelle transport

UR - http://www.scopus.com/inward/record.url?scp=0027231970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027231970&partnerID=8YFLogxK

M3 - Article

C2 - 8496962

AN - SCOPUS:0027231970

VL - 231

SP - 155

EP - 158

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 1

ER -