Sequence relationships of United States prototype and wild-type bluetongue virus RNA genomes investigated by northern blot hybridization analysis.

K. R. Squire, R. Y. Chuang, L. F. Chuang, R. H. Doi, Bennie Osburn

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The 10 double-stranded RNA (dsRNA) genome segments of various isolates of bluetongue virus (BTV) were separated on a polyacrylamide gel, denatured in NaOH, and blotted onto 2-aminophenylthioether paper. Blotted dsRNA segments were detected, using radioactive probes, a cloned copy of DNA 70% fragment of genome segment 7 of BTV-17, whole genome BTV-17 copy DNA, or whole genome BTV-17 dsRNA. These probes detected sequence diversities in different isolates of BTV and these diversities are discussed in relation to the serotype and the electrophoretic migration patterns of the isolates.

Original languageEnglish (US)
Pages (from-to)53-60
Number of pages8
JournalAmerican Journal of Veterinary Research
Volume47
Issue number1
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Bluetongue virus
prototypes
Northern blotting
Northern Blotting
hybridization
Genome
RNA
Double-Stranded RNA
double-stranded RNA
genome
DNA
polyacrylamide
serotypes
gels

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Sequence relationships of United States prototype and wild-type bluetongue virus RNA genomes investigated by northern blot hybridization analysis. / Squire, K. R.; Chuang, R. Y.; Chuang, L. F.; Doi, R. H.; Osburn, Bennie.

In: American Journal of Veterinary Research, Vol. 47, No. 1, 01.01.1986, p. 53-60.

Research output: Contribution to journalArticle

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