Abstract
A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 203-213 |
| Number of pages | 11 |
| Journal | Basic Life Sciences |
| Volume | 40 |
| State | Published - 1986 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Medicine(all)
Cite this
Sequence of the M1-2 region of killer virus double-stranded RNA. / Georgopoulos, D. E.; Hannig, E. M.; Leibowitz, Michael J.
In: Basic Life Sciences, Vol. 40, 1986, p. 203-213.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sequence of the M1-2 region of killer virus double-stranded RNA.
AU - Georgopoulos, D. E.
AU - Hannig, E. M.
AU - Leibowitz, Michael J
PY - 1986
Y1 - 1986
N2 - A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.
AB - A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.
UR - http://www.scopus.com/inward/record.url?scp=0022855428&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022855428&partnerID=8YFLogxK
M3 - Article
C2 - 3551914
AN - SCOPUS:0022855428
VL - 40
SP - 203
EP - 213
JO - Basic Life Sciences
JF - Basic Life Sciences
SN - 0090-5542
ER -