Abstract
A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 203-213 |
| Number of pages | 11 |
| Journal | Basic Life Sciences |
| Volume | 40 |
| State | Published - 1986 |
| Externally published | Yes |
ASJC Scopus subject areas
- Medicine(all)