Sequence of the M1-2 region of killer virus double-stranded RNA.

D. E. Georgopoulos, E. M. Hannig, Michael J Leibowitz

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.

Original languageEnglish (US)
Pages (from-to)203-213
Number of pages11
JournalBasic Life Sciences
Volume40
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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