Sequence of the M1-2 region of killer virus double-stranded RNA.

D. E. Georgopoulos, E. M. Hannig, Michael J Leibowitz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.

Original languageEnglish (US)
Pages (from-to)203-213
Number of pages11
JournalBasic Life Sciences
Volume40
StatePublished - 1986
Externally publishedYes

Fingerprint

Double-Stranded RNA
Viruses
Open Reading Frames
Reverse Transcription
Complementary DNA
Yeasts
Genome
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Sequence of the M1-2 region of killer virus double-stranded RNA. / Georgopoulos, D. E.; Hannig, E. M.; Leibowitz, Michael J.

In: Basic Life Sciences, Vol. 40, 1986, p. 203-213.

Research output: Contribution to journalArticle

Georgopoulos, D. E. ; Hannig, E. M. ; Leibowitz, Michael J. / Sequence of the M1-2 region of killer virus double-stranded RNA. In: Basic Life Sciences. 1986 ; Vol. 40. pp. 203-213.
@article{9a5ec07014004faa81545d95d2c88b15,
title = "Sequence of the M1-2 region of killer virus double-stranded RNA.",
abstract = "A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.",
author = "Georgopoulos, {D. E.} and Hannig, {E. M.} and Leibowitz, {Michael J}",
year = "1986",
language = "English (US)",
volume = "40",
pages = "203--213",
journal = "Basic Life Sciences",
issn = "0090-5542",
publisher = "Springer GmbH & Co, Auslieferungs-Gesellschaf",

}

TY - JOUR

T1 - Sequence of the M1-2 region of killer virus double-stranded RNA.

AU - Georgopoulos, D. E.

AU - Hannig, E. M.

AU - Leibowitz, Michael J

PY - 1986

Y1 - 1986

N2 - A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.

AB - A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.

UR - http://www.scopus.com/inward/record.url?scp=0022855428&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022855428&partnerID=8YFLogxK

M3 - Article

C2 - 3551914

AN - SCOPUS:0022855428

VL - 40

SP - 203

EP - 213

JO - Basic Life Sciences

JF - Basic Life Sciences

SN - 0090-5542

ER -